################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BbvCI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MunI MwoI NaeI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I SspBI SspI StuI SwaI TfiI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AfaI 244 GTAC GT^AC ApaI 394 GGGCCC GGGCC^C AspI 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 200 CCCGAG C^[CT]CG[GA]G BalI 520 TGGCCA TGG^CCA BanI 111 GGCGCC G^G[CT][GA]CC BanII 394 GGGCCC G[GA]GC[CT]^C BbeI 111 GGCGCC GGCGC^C BcgI 202 CGAGACTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 986 GTATCC GTATCC(6/5) BclI 720 TGATCA T^GATCA BmyI 394 GGGCCC G[AGT]GC[ACT]^C BpmI 604 CTGGAG CTGGAG(16/14) BsaAI 214 TACGTA [CT]AC^GT[GA] BsaI 195 GGTCTC GGTCTC(1/5) BseRI 322 CTCCTC GAGGAG(10/8) BsoBI 200 CCCGAG C^[CT]CG[GA]G Bsp1286I 394 GGGCCC G[AGT]GC[ACT]^C BspMI 545 ACCTGC ACCTGC(4/8) BsrDI 846 CATTGC GCAATG(2/0) BstYI 968 AGATCC [GA]^GATC[CT] CfoI 112 GCGC GCG^C DraIII 524 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1063 GACATGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 520 TGGCCA [CT]^GGCC[GA] EcoT22I 949 ATGCAT ATGCA^T FauI 236 GCGGG CCCGC(4/6) FbaI 720 TGATCA T^GATCA HaeII 111 GGCGCC [GA]GCGC^[CT] HgaI 230 GCGTC GACGC(5/10) HhaI 112 GCGC GCG^C HinP1I 112 GCGC G^CGC KasI 111 GGCGCC G^GCGCC KspI 234 CCGCGG CCGC^GG MaeII 215 ACGT A^CGT MflI 968 AGATCC [GA]^GATC[CT] MluNI 520 TGGCCA TGG^CCA MscI 520 TGGCCA TGG^CCA MslI 1008 CATTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 111 GGCGCC GG^CGCC NruI 171 TCGCGA TCG^CGA NsiI 949 ATGCAT ATGCA^T NspI 951 GCATGC [GA]CATG^[CT] PflFI 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 90 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 821 GGGACCT [GA]G^G[AT]CC[CT] PstI 313 CTGCAG CTGCA^G PvuII 376 CAGCTG CAG^CTG RsaI 244 GTAC GT^AC SacII 234 CCGCGG CCGC^GG SfcI 313 CTGCAG C^T[GA][CT]AG SfoI 111 GGCGCC GGC^GCC SmlI 633 CTCAAG C^T[CT][GA]AG SnaBI 214 TACGTA TAC^GTA SphI 951 GCATGC GCATG^C TaqI 1073 TCGA T^CGA Tth111I 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1073 TCGA T^CGA Van91I 90 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 854 CCAAGGGGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 968 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 172 CGCG CG^CG 235 CGCG AcyI 111 GGCGCC G[GA]^CG[CT]C 229 GGCGTC Asp700I 156 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 585 GAAAAATTTC AvaII 418 GGACC G^G[AT]CC 822 GGACC BbsI 655 GAAGAC GAAGAC(2/6) 1060 GAAGAC BcnI 581 CCCGG CC^[GC]GG 819 CCGGG BpuAI 655 GAAGAC GAAGAC(2/6) 1060 GAAGAC BsaHI 111 GGCGCC G[GA]^CG[CT]C 229 GGCGTC BstF5I 756 CATCC GGATG(2/0) 552 GGATG BstUI 172 CGCG CG^CG 235 CGCG DraII 393 GGGGCCC [GA]G^G[ACGT]CC[CT] 821 GGGACCT EcoNI 444 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 481 CCTAAGCCAGG EcoO109I 393 GGGGCCC [GA]G^G[ACGT]CC[CT] 821 GGGACCT FokI 756 CATCC GGATG(9/13) 552 GGATG HapII 582 CCGG C^CGG 819 CCGG Hin1I 111 GGCGCC G[GA]^CG[CT]C 229 GGCGTC HincII 363 GTTGAC GT[CT]^[GA]AC 958 GTTGAC HindII 363 GTTGAC GT[CT]^[GA]AC 958 GTTGAC HindIII 299 AAGCTT A^AGCTT 769 AAGCTT HinfI 631 GACTC G^A[ACGT]TC 708 GACTC HpaII 582 CCGG C^CGG 819 CCGG MaeIII 706 GTGAC ^GT[ACGT]AC 797 GTCAC MspA1I 234 CCGCGG C[AC]G^C[GT]G 376 CAGCTG MspI 582 CCGG C^CGG 819 CCGG MvnI 172 CGCG CG^CG 235 CGCG NciI 581 CCCGG CC^[GC]GG 819 CCGGG NcoI 517 CCATGG C^CATGG 879 CCATGG PleI 631 GACTC GAGTC(4/5) 708 GACTC SfaNI 677 GATGC GCATC(5/9) 948 GATGC Tsp45I 706 GTGAC ^GT[GC]AC 797 GTCAC XmnI 156 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 585 GAAAAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI HaeIII HphI ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp509I TspRI