(Circular) (Six-base) MAPSORT of: pFN21AE3610.seq Check: 8808 from: 1 to: 5797 pFN21AE3610 5797 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2679 3860 5244 5512 1632 Size: 1047 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1181 1047 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2896 1184 Size: 1712 4085 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2403 2403 Size: 5797 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 2163 2295 2593 2647 3106 3332 3484 5593 Size: 670 132 298 54 459 226 152 2109 Cuts at: 5593 1493 Size: 1697 Fragments arranged by size: 2109 1697 670 459 298 226 152 132 54 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2205 3122 1870 Size: 73 262 917 4545 Fragments arranged by size: 4545 917 262 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 3118 4573 618 Size: 325 217 627 329 1002 1455 1842 Fragments arranged by size: 1842 1455 1002 627 329 325 217 BanII G_rGCy'C Cuts at: 1391 2403 3116 1391 Size: 1012 713 4072 Fragments arranged by size: 4072 1012 713 BbeI G_GCGC'C Cuts at: 2120 2120 Size: 5797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2667 3072 928 Size: 971 768 405 3653 Fragments arranged by size: 3653 971 768 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2196 2230 3988 4022 1903 Size: 34 50 34 175 34 1758 34 3678 Fragments arranged by size: 3678 1758 175 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3002 3689 4899 3002 Size: 687 1210 3900 Fragments arranged by size: 3900 1210 687 BclI T'GATC_A Cuts at: 1548 2725 1548 Size: 1177 4620 BfrBI ATG'CAT Cuts at: 2956 2956 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2403 3858 5014 1790 Size: 613 1455 1156 2573 Fragments arranged by size: 2573 1455 1156 613 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2205 3528 4486 Size: 416 53 83 399 24 788 1323 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1323 958 788 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 4457 1239 Size: 1391 1827 2579 Fragments arranged by size: 2579 1827 1391 Bpu10I CC'TnA_GC Cuts at: 2487 2565 2487 Size: 78 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2623 3922 4787 5085 2623 Size: 1299 865 298 3335 Fragments arranged by size: 3335 1299 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2206 4460 882 Size: 617 707 2254 2219 Fragments arranged by size: 2254 2219 707 617 BsaAI yAC'GTr Cuts at: 493 1204 2221 3588 493 Size: 711 1017 1367 2702 Fragments arranged by size: 2702 1367 1017 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2117 2235 3604 3986 Size: 53 83 186 936 584 118 1369 382 Cuts at: 3986 4621 275 Size: 635 1451 Fragments arranged by size: 1451 1369 936 635 584 382 186 118 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 1186 Size: 1132 4665 BseYI C'CCAG_C Cuts at: 2230 2379 2383 5000 5496 2230 Size: 149 4 2617 496 2531 Fragments arranged by size: 2617 2531 496 149 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3858 3943 5014 3858 Size: 85 1071 4641 Fragments arranged by size: 4641 1071 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 3116 3858 3943 5014 1391 Size: 399 613 713 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 613 399 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 3152 844 Size: 1715 593 3489 Fragments arranged by size: 3489 1715 593 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2850 4292 4466 65 Size: 1183 1602 1442 174 1396 Fragments arranged by size: 1602 1442 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2973 3127 3494 3879 3896 5348 5434 5446 Size: 1001 154 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 385 367 346 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2239 2522 2884 513 Size: 552 1174 283 362 3426 Fragments arranged by size: 3426 1174 552 362 283 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 2534 2534 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3074 4804 809 Size: 507 1758 1730 1802 Fragments arranged by size: 1802 1758 1730 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2525 3244 4133 Size: 54 1425 50 275 18 695 719 889 Cuts at: 4133 5762 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 719 695 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2630 2679 3860 4457 5244 5512 1239 Size: 393 998 49 1181 597 787 268 1524 Fragments arranged by size: 1524 1181 998 787 597 393 268 49 EcoNI CCTnn'n_nnAGG Cuts at: 2453 2490 2453 Size: 37 5760 EcoO109I rG'GnC_Cy Cuts at: 2399 2827 2399 Size: 428 5369 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2120 4944 1678 Size: 442 2824 2531 Fragments arranged by size: 2824 2531 442 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 2745 2777 2899 Size: 32 135 32 1049 32 92 32 122 Cuts at: 2899 2931 4438 4470 4512 4544 1373 Size: 32 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 1049 135 122 92 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2370 2965 3141 3387 669 Size: 988 281 432 595 176 246 3079 Fragments arranged by size: 3079 988 595 432 281 246 176 HindIII A'AGCT_T Cuts at: 748 2304 2774 748 Size: 1556 470 3771 Fragments arranged by size: 3771 1556 470 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2116 2116 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2161 4911 5095 1305 Size: 856 2750 184 2007 Fragments arranged by size: 2750 2007 856 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2527 10 Size: 54 1425 343 695 3280 Fragments arranged by size: 3280 1425 695 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3755 4114 4273 518 Size: 648 1851 738 359 159 2042 Fragments arranged by size: 2042 1851 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2241 2383 3213 3890 5038 5283 1606 Size: 635 142 830 677 1148 245 2120 Fragments arranged by size: 2120 1148 830 677 635 245 142 NarI GG'CG_CC Cuts at: 2117 2117 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 2522 2884 513 Size: 552 1457 362 3426 Fragments arranged by size: 3426 1457 552 362 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NruI TCG'CGA Cuts at: 2178 2178 Size: 5797 NsiI A_TGCA'T Cuts at: 2958 2958 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2960 3155 4700 1132 Size: 97 1731 195 1545 2229 Fragments arranged by size: 2229 1731 1545 195 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 1108 Size: 993 4804 PfoI T'CCnGG_A Cuts at: 2169 2607 2823 2169 Size: 438 216 5143 Fragments arranged by size: 5143 438 216 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 2827 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2399 2399 Size: 5797 PstI C_TGCA'G Cuts at: 830 2322 3149 830 Size: 1492 827 3478 Fragments arranged by size: 3478 1492 827 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2383 2383 Size: 5797 SacII CC_GC'GG Cuts at: 2242 2242 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2318 3145 4280 4961 5152 826 Size: 219 1273 827 1135 681 191 1471 Fragments arranged by size: 1471 1273 1135 827 681 219 191 SfoI GGC'GCC Cuts at: 2118 2118 Size: 5797 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 3901 4802 5064 820 Size: 197 926 695 1263 901 262 1553 Fragments arranged by size: 1553 1263 926 901 695 262 197 SnaBI TAC'GTA Cuts at: 493 2221 3588 493 Size: 1728 1367 2702 Fragments arranged by size: 2702 1728 1367 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 2960 3155 1132 Size: 1828 195 3774 Fragments arranged by size: 3774 1828 195 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2269 2522 2859 2884 3236 513 Size: 552 1204 253 337 25 352 3074 Fragments arranged by size: 3074 1204 552 352 337 253 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3078 3824 3841 3994 4179 4624 325 Size: 1255 1498 746 17 153 185 445 1498 Fragments arranged by size: 1498 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 4043 5755 Size: 274 80 33 51 1443 271 1795 1712 Cuts at: 5755 96 Size: 138 Fragments arranged by size: 1795 1712 1443 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 3044 1316 Size: 1728 4069 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 2165 2594 3926 2165 Size: 429 1332 4036 Fragments arranged by size: 4036 1332 429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI