################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## Acc65I AccI AccII AccIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII BalI BamHI BbrPI BbvCI BcnI BglII BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FseI FspI HapII HgaI HincII HindII HpaI HpaII KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 25 GACGTC GACGT^C AflII 439 CTTAAG C^TTAAG AflIII 1791 ACGTGT A^C[GA][CT]GT AhdI 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1083 GGATC GGATC(4/5) AlwNI 1106 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 628 CCTGAGG CC^T[ACGT]AGG AspEI 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1735 CCTAGG C^CTAGG BanI 3 GGCGCC G^G[CT][GA]CC BbeI 3 GGCGCC GGCGC^C BciVI 486 GGATAC GTATCC(6/5) BclI 1776 TGATCA T^GATCA BfrI 439 CTTAAG C^TTAAG BglI 2060 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1735 CCTAGG C^CTAGG BlpI 264 GCTTAGC GC^T[ACGT]AGC Bpu1102I 264 GCTTAGC GC^T[ACGT]AGC BsgI 1922 GTGCAG GTGCAG(16/14) BspHI 855 TCATGA T^CATGA BsrBI 2061 CCGCTC CCGCTC(-3/-3) BstYI 1083 GGATCT [GA]^GATC[CT] Bsu36I 628 CCTGAGG CC^T[ACGT]AGG CelII 264 GCTTAGC GC^T[ACGT]AGC CfoI 4 GCGC GCG^C DraI 550 TTTAAA TTT^AAA Eam1105I 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 628 CCTGAGG CC^T[ACGT]AGG EcoRI 1226 GAATTC G^AATTC EcoT22I 166 ATGCAT ATGCA^T FauI 784 CCCGC CCCGC(4/6) FbaI 1776 TGATCA T^GATCA HaeII 3 GGCGCC [GA]GCGC^[CT] HhaI 4 GCGC GCG^C HinP1I 4 GCGC G^CGC HindIII 82 AAGCTT A^AGCTT KasI 3 GGCGCC G^GCGCC MfeI 911 CAATTG C^AATTG MflI 1083 GGATCT [GA]^GATC[CT] MunI 911 CAATTG C^AATTG NarI 3 GGCGCC GG^CGCC NdeI 977 CATATG CA^TATG NsiI 166 ATGCAT ATGCA^T NspI 1948 GCATGT [GA]CATG^[CT] PflMI 976 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 855 TCATGA T^CATGA SacI 1217 GAGCTC GAGCT^C SbfI 1929 CCTGCAGG CCTGCA^GG SfoI 3 GGCGCC GGC^GCC SpeI 1588 ACTAGT A^CTAGT Sse8387I 1929 CCTGCAGG CCTGCA^GG StuI 1926 AGGCCT AGG^CCT Van91I 976 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1083 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AclI 941 AACGTT AA^CGTT 967 AACGTT AcyI 3 GGCGCC G[GA]^CG[CT]C 25 GACGTC AvaII 781 GGTCC G^G[AT]CC 1999 GGTCC BanII 67 GGGCTC G[GA]GC[CT]^C 1217 GAGCTC BbsI 28 GTCTTC GAAGAC(2/6) 1151 GTCTTC BcgI 114 CGATTTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2050 CGAGCCTGCTGC BpmI 1619 CTCCAG CTGGAG(16/14) 1072 CTGGAG BpuAI 28 GTCTTC GAAGAC(2/6) 1151 GTCTTC BsaHI 3 GGCGCC G[GA]^CG[CT]C 25 GACGTC BsaI 704 GGTCTC GGTCTC(1/5) 1843 GGTCTC BseRI 391 GAGGAG GAGGAG(10/8) 1865 GAGGAG BsiYI 38 CCCCACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 976 CCATATGTTGG BslI 38 CCCCACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 976 CCATATGTTGG BsmFI 282 GGGAC GGGAC(10/14) 782 GTCCC Bsp1407I 234 TGTACA T^GTACA 1654 TGTACA BsrDI 924 CATTGC GCAATG(2/0) 1702 GCAATG BsrGI 234 TGTACA T^GTACA 1654 TGTACA BssKI 1071 CCTGG ^CC[ACGT]GG 1391 CCTGG BstNI 1071 CCTGG CC^[AT]GG 1391 CCTGG BstXI 407 CCATGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1031 CCAGCAAAATGG EcoRII 1071 CCTGG ^CC[AT]GG 1391 CCTGG Hin1I 3 GGCGCC G[GA]^CG[CT]C 25 GACGTC MspA1I 58 CAGCTG C[AC]G^C[GT]G 709 CAGCTG MvaI 1071 CCTGG CC^[AT]GG 1391 CCTGG NcoI 951 CCATGG C^CATGG 1839 CCATGG NlaIV 3 GGCGCC GG[ACGT]^[ACGT]CC 781 GGTCCC PshAI 1587 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1837 GACCATGGTC Psp1406I 941 AACGTT AA^CGTT 967 AACGTT PvuII 58 CAGCTG CAG^CTG 709 CAGCTG ScrFI 1071 CCTGG CC^[ACGT]GG 1391 CCTGG SmlI 439 CTTAAG C^T[CT][GA]AG 665 CTCAAG SspBI 234 TGTACA T^GTACA 1654 TGTACA SspI 419 AATATT AAT^ATT 554 AATATT TaqI 678 TCGA T^CGA 2049 TCGA TthHB8I 678 TCGA T^CGA 2049 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BbvI BfaI BmyI BsaJI BsiHKAI BsmAI Bsp1286I BsrI BstF5I Cac8I Cfr13I DdeI DpnII DsaI EarI EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII PleI PstI RsaI SapI Sau3AI Sau96I SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI