(Circular) (Six-base) MAPSORT of: pFN21AE3518.seq Check: 6975 from: 1 to: 6778 pFN21AE3518 6778 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2034 4588 278 Size: 53 83 186 936 498 2554 2468 Fragments arranged by size: 2554 2468 936 498 186 83 53 AccI GT'mk_AC Cuts at: 1937 4121 1937 Size: 2184 4594 Acc65I G'GTAC_C Cuts at: 4099 4099 Size: 6778 AclI AA'CG_TT Cuts at: 1563 2947 2973 4905 5278 1563 Size: 1384 26 1932 373 3063 Fragments arranged by size: 3063 1932 1384 373 26 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 2312 2556 2916 3477 4841 6225 6493 Size: 408 272 244 360 561 1364 1384 268 Cuts at: 6493 1632 Size: 1917 Fragments arranged by size: 1917 1384 1364 561 408 360 272 268 244 AflII C'TTAA_G Cuts at: 820 1017 2444 820 Size: 197 1427 5154 Fragments arranged by size: 5154 1427 197 AflIII A'CryG_T Cuts at: 1203 3796 5677 6654 1203 Size: 2593 1881 977 1327 Fragments arranged by size: 2593 1881 1327 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 3130 5507 3130 Size: 2377 4401 AleI CACnn'nnGTG Cuts at: 1166 3197 1166 Size: 2031 4747 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2494 3633 1184 Size: 1310 1139 4329 Fragments arranged by size: 4329 1310 1139 AlwNI CAG_nnn'CTG Cuts at: 3116 4521 6093 3116 Size: 1405 1572 3801 Fragments arranged by size: 3801 1572 1405 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 3231 3421 4036 4087 4313 4465 6574 1493 Size: 1738 190 615 51 226 152 2109 1697 Fragments arranged by size: 2109 1738 1697 615 226 190 152 51 AseI AT'TA_AT Cuts at: 160 1032 5332 160 Size: 872 4300 1606 Fragments arranged by size: 4300 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 4103 1870 Size: 73 2160 4545 Fragments arranged by size: 4545 2160 73 AvrII C'CTAG_G Cuts at: 3740 3740 Size: 6778 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2982 3015 2982 Size: 33 6745 BamHI G'GATC_C Cuts at: 4108 4475 4108 Size: 367 6411 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 4099 5554 618 Size: 325 217 627 221 2091 1455 1842 Fragments arranged by size: 2091 1842 1455 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2076 3226 4097 1391 Size: 685 1150 871 4072 Fragments arranged by size: 4072 1150 871 685 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6778 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2026 3149 928 Size: 971 127 1123 4557 Fragments arranged by size: 4557 1123 971 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2108 2142 4044 4078 4969 5003 1903 Size: 34 171 34 1902 34 891 34 3678 Fragments arranged by size: 3678 1902 891 171 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2485 4670 5880 2485 Size: 2185 1210 3383 Fragments arranged by size: 3383 2185 1210 BclI T'GATC_A Cuts at: 1548 3781 1548 Size: 2233 4545 BfrBI ATG'CAT Cuts at: 2173 2173 Size: 6778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4071 5389 136 Size: 107 122 71 631 605 2399 1318 1525 Fragments arranged by size: 2399 1525 1318 631 605 122 107 71 BglII A'GATC_T Cuts at: 6773 6773 Size: 6778 BlpI GC'TnA_GC Cuts at: 2270 4195 2270 Size: 1925 4853 Bme1580I G_kGCm'C Cuts at: 1790 4839 5995 1790 Size: 3049 1156 2573 Fragments arranged by size: 3049 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3797 1318 Size: 2479 4299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4509 5467 442 Size: 416 53 83 399 24 3092 958 1753 Fragments arranged by size: 3092 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3098 3609 5438 1239 Size: 1859 511 1829 2579 Fragments arranged by size: 2579 1859 1829 511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2655 4903 5768 6066 2655 Size: 2248 865 298 3367 Fragments arranged by size: 3367 2248 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2715 3854 5441 882 Size: 617 1216 1139 1587 2219 Fragments arranged by size: 2219 1587 1216 1139 617 BsaAI yAC'GTr Cuts at: 493 1204 4569 493 Size: 711 3365 2702 Fragments arranged by size: 3365 2702 711 BsaBI GATnn'nnATC Cuts at: 6772 6772 Size: 6778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2031 4585 4967 Size: 53 83 186 936 476 22 2554 382 Cuts at: 4967 5602 275 Size: 635 1451 Fragments arranged by size: 2554 1451 936 635 476 382 186 83 53 22 BsaWI w'CCGG_w Cuts at: 5211 5609 5883 6030 6441 5211 Size: 398 274 147 411 5548 Fragments arranged by size: 5548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2937 2967 1178 Size: 30 165 30 1534 30 4989 Fragments arranged by size: 4989 1534 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 3885 1186 Size: 1225 1474 4079 Fragments arranged by size: 4079 1474 1225 BseYI C'CCAG_C Cuts at: 5981 6477 5981 Size: 496 6282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3948 1163 Size: 573 2212 3993 Fragments arranged by size: 3993 2212 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2131 3226 3894 4839 4924 5995 2131 Size: 1095 668 945 85 1071 2914 Fragments arranged by size: 2914 1095 1071 945 668 85 BsmI GAATG_Cn' Cuts at: 4289 4382 4289 Size: 93 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2131 3226 3894 4097 4839 4924 Size: 399 286 55 1095 668 203 742 85 Cuts at: 4924 5995 1391 Size: 1071 2174 Fragments arranged by size: 2174 1095 1071 742 668 399 286 203 85 55 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 2860 4667 6397 2860 Size: 1807 1730 3241 Fragments arranged by size: 3241 1807 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4133 844 Size: 3289 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4068 4665 1394 Size: 429 2245 597 3507 Fragments arranged by size: 3507 2245 597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2928 3714 5273 5447 65 Size: 1183 1680 786 1559 174 1396 Fragments arranged by size: 1680 1559 1396 1183 786 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5422 5609 1700 Size: 110 3612 187 2869 Fragments arranged by size: 3612 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2239 3659 96 Size: 2143 1420 3215 Fragments arranged by size: 3215 2143 1420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6778 BstBI TT'CG_AA Cuts at: 5582 5582 Size: 6778 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6778 BstXI CCAn_nnnn'nTGG Cuts at: 2419 3043 2419 Size: 624 6154 BstYI r'GATC_y Cuts at: 1972 3088 4108 4475 4860 4877 6329 6415 6427 Size: 1116 1020 367 385 17 1452 86 12 Cuts at: 6427 6773 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1116 1020 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2634 2634 Size: 6778 BtgI C'CryG_G Cuts at: 513 1065 2004 2565 2956 3844 513 Size: 552 939 561 391 888 3447 Fragments arranged by size: 3447 939 888 561 552 391 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 2476 4049 4294 5088 5108 2476 Size: 1573 245 794 20 4146 Fragments arranged by size: 4146 1573 794 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6743 DraI TTT'AAA Cuts at: 2557 4082 2557 Size: 1525 5253 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4225 5114 6743 Size: 54 1425 50 275 18 2395 889 1629 Cuts at: 6743 8 Size: 43 Fragments arranged by size: 2395 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2388 2433 2532 2892 4708 1353 Size: 783 252 45 99 360 1816 3423 Fragments arranged by size: 3423 1816 783 360 252 99 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2068 5365 5739 5885 387 Size: 1064 138 479 3297 374 146 1280 Fragments arranged by size: 3297 1280 1064 479 374 146 138 EcoICRI GAG'CTC Cuts at: 3224 3224 Size: 6778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2312 2556 2916 3098 3477 3609 Size: 393 408 272 244 360 182 379 132 Cuts at: 3609 4841 5438 6225 6493 1239 Size: 1232 597 787 268 1524 Fragments arranged by size: 1524 1232 787 597 408 393 379 360 272 268 244 182 132 EcoRI G'AATT_C Cuts at: 3231 4087 4465 3231 Size: 856 378 5544 Fragments arranged by size: 5544 856 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2871 2871 Size: 6778 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 2012 5925 1678 Size: 334 3913 2531 Fragments arranged by size: 3913 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 3116 3148 3229 Size: 32 135 32 989 32 523 32 81 Cuts at: 3229 3261 5419 5451 5493 5525 1373 Size: 32 2158 32 42 32 2626 Fragments arranged by size: 2626 2158 989 523 135 81 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4122 4368 669 Size: 988 281 2184 246 3079 Fragments arranged by size: 3079 2184 988 281 246 HindIII A'AGCT_T Cuts at: 748 2087 748 Size: 1339 5439 HpaI GTT'AAC Cuts at: 4368 4368 Size: 6778 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6778 KpnI G_GTAC'C Cuts at: 4103 4103 Size: 6778 MfeI C'AATT_G Cuts at: 2916 4377 2916 Size: 1461 5317 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 3561 3562 5892 6076 1305 Size: 828 1428 1 2330 184 2007 Fragments arranged by size: 2330 2007 1428 828 184 1 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4956 Fragments arranged by size: 4956 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 3197 3249 3314 3501 4736 5095 Size: 648 1251 780 52 65 187 1235 359 Cuts at: 5095 5254 518 Size: 159 2042 Fragments arranged by size: 2042 1251 1235 780 648 359 187 159 65 52 MspA1I CmG'CkG Cuts at: 1606 2065 2716 4194 4871 6019 6264 1606 Size: 459 651 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 651 459 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6778 NcoI C'CATG_G Cuts at: 513 1065 2004 2956 3844 513 Size: 552 939 952 888 3447 Fragments arranged by size: 3447 952 939 888 552 NdeI CA'TA_TG Cuts at: 387 2983 387 Size: 2596 4182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NsiI A_TGCA'T Cuts at: 2175 2175 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 3957 4136 5681 1132 Size: 97 2728 179 1545 2229 Fragments arranged by size: 2728 2229 1545 179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6778 PciI A'CATG_T Cuts at: 5677 5677 Size: 6778 PflMI CCAn_nnn'nTGG Cuts at: 1108 2987 1108 Size: 1879 4899 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4847 4879 6360 6392 4847 Size: 32 1481 32 5233 Fragments arranged by size: 5233 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3596 3846 1613 Size: 1983 250 4545 Fragments arranged by size: 4545 1983 250 PsiI TTA'TAA Cuts at: 2235 4348 2235 Size: 2113 4665 PstI C_TGCA'G Cuts at: 830 2139 2500 3031 3939 4130 830 Size: 1309 361 531 908 191 3478 Fragments arranged by size: 3478 1309 908 531 361 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 PvuII CAG'CTG Cuts at: 2065 2716 2065 Size: 651 6127 SacI G_AGCT'C Cuts at: 3226 3226 Size: 6778 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SapI GCTCTTCn'nnn_ Cuts at: 2136 2433 2532 2136 Size: 297 99 6382 Fragments arranged by size: 6382 297 99 SbfI CC_TGCA'GG Cuts at: 3939 4130 3939 Size: 191 6587 ScaI AGT'ACT Cuts at: 5026 6738 5026 Size: 1712 5066 SfcI C'TryA_G Cuts at: 826 1045 2135 2496 3027 3935 4126 5261 5942 Size: 219 1090 361 531 908 191 1135 681 Cuts at: 5942 6133 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1090 908 681 531 361 219 191 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6778 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 4105 1872 Size: 2233 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2670 4882 5783 6045 820 Size: 197 926 501 226 2212 901 262 1553 Fragments arranged by size: 2212 1553 926 901 501 262 226 197 SnaBI TAC'GTA Cuts at: 493 4569 493 Size: 4076 2702 SpeI A'CTAG_T Cuts at: 152 3593 152 Size: 3441 3337 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 2426 2561 4702 5 Size: 47 2374 135 2141 2081 Fragments arranged by size: 2374 2141 2081 135 47 StuI AGG'CCT Cuts at: 1517 3933 1517 Size: 2416 4362 StyI C'CwwG_G Cuts at: 513 1065 2004 2956 3740 3844 4217 513 Size: 552 939 952 784 104 373 3074 Fragments arranged by size: 3074 952 939 784 552 373 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2815 4805 4822 4975 5160 5605 325 Size: 1255 1235 1990 17 153 185 445 1498 Fragments arranged by size: 1990 1498 1255 1235 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2239 3659 3799 Size: 274 80 33 51 1443 262 1420 140 Cuts at: 3799 5024 6736 96 Size: 1225 1712 138 Fragments arranged by size: 1712 1443 1420 1225 274 262 140 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6738 6770 6738 Size: 32 6746 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6778 XmaI C'CCGG_G Cuts at: 1870 4103 1870 Size: 2233 4545 XmnI GAAnn'nnTTC Cuts at: 4907 4907 Size: 6778 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2032 4586 276 Size: 53 83 186 936 498 2554 2468 Fragments arranged by size: 2554 2468 936 498 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI