(Linear) (Six-base) MAPSORT of: KIEE3518.seq Check: 6130 from: 1 to: 2073 KIEE3518.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AatII G_ACGT'C Cuts at: 0 29 2073 Size: 29 2044 AclI AA'CG_TT Cuts at: 0 942 968 2073 Size: 942 26 1105 Fragments arranged by size: 1105 942 26 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 307 551 911 1472 2073 Size: 35 272 244 360 561 601 Fragments arranged by size: 601 561 360 272 244 35 AflII C'TTAA_G Cuts at: 0 439 2073 Size: 439 1634 AflIII A'CryG_T Cuts at: 0 1791 2073 Size: 1791 282 AhdI GACnn_n'nnGTC Cuts at: 0 1125 2073 Size: 1125 948 AleI CACnn'nnGTG Cuts at: 0 1192 2073 Size: 1192 881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 489 1628 2073 Size: 489 1139 445 Fragments arranged by size: 1139 489 445 AlwNI CAG_nnn'CTG Cuts at: 0 1111 2073 Size: 1111 962 ApoI r'AATT_y Cuts at: 0 1226 1416 2031 2073 Size: 1226 190 615 42 Fragments arranged by size: 1226 615 190 42 AvrII C'CTAG_G Cuts at: 0 1735 2073 Size: 1735 338 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 977 1010 2073 Size: 977 33 1063 Fragments arranged by size: 1063 977 33 BanI G'GyrC_C Cuts at: 0 3 2073 Size: 3 2070 BanII G_rGCy'C Cuts at: 0 71 1221 2073 Size: 71 1150 852 Fragments arranged by size: 1150 852 71 BbeI G_GCGC'C Cuts at: 0 7 2073 Size: 7 2066 BbsI GAAGACnn'nnnn_ Cuts at: 0 21 1144 2073 Size: 21 1123 929 Fragments arranged by size: 1123 929 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 103 137 2039 2073 Size: 103 34 1902 34 Fragments arranged by size: 1902 103 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 480 2073 Size: 480 1593 BclI T'GATC_A Cuts at: 0 1776 2073 Size: 1776 297 BfrBI ATG'CAT Cuts at: 0 168 2073 Size: 168 1905 BglI GCCn_nnn'nGGC Cuts at: 0 2066 2073 Size: 2066 7 BlpI GC'TnA_GC Cuts at: 0 265 2073 Size: 265 1808 BmgBI CAC'GTC Cuts at: 0 1792 2073 Size: 1792 281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1093 1604 2073 Size: 1093 511 469 Fragments arranged by size: 1093 511 469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 650 2073 Size: 650 1423 BsaI GGTCTCn'nnnn_ Cuts at: 0 710 1849 2073 Size: 710 1139 224 Fragments arranged by size: 1139 710 224 BsaHI Gr'CG_yC Cuts at: 0 4 26 2073 Size: 4 22 2047 Fragments arranged by size: 2047 22 4 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 932 962 2073 Size: 932 30 1111 Fragments arranged by size: 1111 932 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 406 1880 2073 Size: 406 1474 193 Fragments arranged by size: 1474 406 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1943 2073 Size: 1943 130 BsiHKAI G_wGCw'C Cuts at: 0 126 1221 1889 2073 Size: 126 1095 668 184 Fragments arranged by size: 1095 668 184 126 Bsp1286I G_dGCh'C Cuts at: 0 71 126 1221 1889 2073 Size: 71 55 1095 668 184 Fragments arranged by size: 1095 668 184 71 55 BspHI T'CATG_A Cuts at: 0 855 2073 Size: 855 1218 BsrBI CCG'CTC Cuts at: 0 2063 2073 Size: 2063 10 BsrDI GCAATG_nn' Cuts at: 0 923 1709 2073 Size: 923 786 364 Fragments arranged by size: 923 786 364 BsrGI T'GTAC_A Cuts at: 0 234 1654 2073 Size: 234 1420 419 Fragments arranged by size: 1420 419 234 BstXI CCAn_nnnn'nTGG Cuts at: 0 414 1038 2073 Size: 414 624 1035 Fragments arranged by size: 1035 624 414 BstYI r'GATC_y Cuts at: 0 1083 2073 Size: 1083 990 Bsu36I CC'TnA_GG Cuts at: 0 629 2073 Size: 629 1444 BtgI C'CryG_G Cuts at: 0 560 951 1839 2073 Size: 560 391 888 234 Fragments arranged by size: 888 560 391 234 BtsI GCAGTG_nn' Cuts at: 0 471 2044 2073 Size: 471 1573 29 Fragments arranged by size: 1573 471 29 DraI TTT'AAA Cuts at: 0 552 2073 Size: 552 1521 EarI CTCTTCn'nnn_ Cuts at: 0 131 383 428 527 887 2073 Size: 131 252 45 99 360 1186 Fragments arranged by size: 1186 360 252 131 99 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 63 2073 Size: 63 2010 EcoICRI GAG'CTC Cuts at: 0 1219 2073 Size: 1219 854 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 307 551 911 1093 1472 1604 2073 Size: 35 272 244 360 182 379 132 469 Fragments arranged by size: 469 379 360 272 244 182 132 35 EcoRI G'AATT_C Cuts at: 0 1226 2073 Size: 1226 847 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 866 2073 Size: 866 1207 HaeII r_GCGC'y Cuts at: 0 7 2073 Size: 7 2066 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 556 588 1111 1143 1224 1256 2073 Size: 556 32 523 32 81 32 817 Fragments arranged by size: 817 556 523 81 32 32 32 HindIII A'AGCT_T Cuts at: 0 82 2073 Size: 82 1991 KasI G'GCGC_C Cuts at: 0 3 2073 Size: 3 2070 MfeI C'AATT_G Cuts at: 0 911 2073 Size: 911 1162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 128 1556 1557 2073 Size: 128 1428 1 516 Fragments arranged by size: 1428 516 128 1 MslI CAynn'nnrTG Cuts at: 0 412 1192 1244 1309 1496 2073 Size: 412 780 52 65 187 577 Fragments arranged by size: 780 577 412 187 65 52 MspA1I CmG'CkG Cuts at: 0 60 711 2073 Size: 60 651 1362 Fragments arranged by size: 1362 651 60 NarI GG'CG_CC Cuts at: 0 4 2073 Size: 4 2069 NcoI C'CATG_G Cuts at: 0 951 1839 2073 Size: 951 888 234 Fragments arranged by size: 951 888 234 NdeI CA'TA_TG Cuts at: 0 978 2073 Size: 978 1095 NsiI A_TGCA'T Cuts at: 0 170 2073 Size: 170 1903 NspI r_CATG'y Cuts at: 0 1952 2073 Size: 1952 121 PflMI CCAn_nnn'nTGG Cuts at: 0 982 2073 Size: 982 1091 PshAI GACnn'nnGTC Cuts at: 0 1591 1841 2073 Size: 1591 250 232 Fragments arranged by size: 1591 250 232 PsiI TTA'TAA Cuts at: 0 230 2073 Size: 230 1843 PstI C_TGCA'G Cuts at: 0 134 495 1026 1934 2073 Size: 134 361 531 908 139 Fragments arranged by size: 908 531 361 139 134 PvuII CAG'CTG Cuts at: 0 60 711 2073 Size: 60 651 1362 Fragments arranged by size: 1362 651 60 SacI G_AGCT'C Cuts at: 0 1221 2073 Size: 1221 852 SapI GCTCTTCn'nnn_ Cuts at: 0 131 428 527 2073 Size: 131 297 99 1546 Fragments arranged by size: 1546 297 131 99 SbfI CC_TGCA'GG Cuts at: 0 1934 2073 Size: 1934 139 SfcI C'TryA_G Cuts at: 0 130 491 1022 1930 2073 Size: 130 361 531 908 143 Fragments arranged by size: 908 531 361 143 130 SfoI GGC'GCC Cuts at: 0 5 2073 Size: 5 2068 SmlI C'TyrA_G Cuts at: 0 439 665 2073 Size: 439 226 1408 Fragments arranged by size: 1408 439 226 SpeI A'CTAG_T Cuts at: 0 1588 2073 Size: 1588 485 SspI AAT'ATT Cuts at: 0 421 556 2073 Size: 421 135 1517 Fragments arranged by size: 1517 421 135 StuI AGG'CCT Cuts at: 0 1928 2073 Size: 1928 145 StyI C'CwwG_G Cuts at: 0 951 1735 1839 2073 Size: 951 784 104 234 Fragments arranged by size: 951 784 234 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 810 2073 Size: 810 1263 TatI w'GTAC_w Cuts at: 0 234 1654 1794 2073 Size: 234 1420 140 279 Fragments arranged by size: 1420 279 234 140 ZraI GAC'GTC Cuts at: 0 27 2073 Size: 27 2046 Enzymes that do cut: AatII AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApoI AvrII BaeI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BpmI BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrGI BstXI BstYI Bsu36I BtgI BtsI DraI EarI EciI EcoICRI Eco57MI EcoRI FalI HaeII Hin4I HindIII KasI MfeI MmeI MslI MspA1I NarI NcoI NdeI NsiI NspI PflMI PshAI PsiI PstI PvuII SacI SapI SbfI SfcI SfoI SmlI SpeI SspI StuI StyI TaqII TatI ZraI Enzymes that do not cut: AarI AccI Acc65I AfeI AgeI AloI ApaI ApaLI AscI AseI AsiSI AvaI BamHI BbvCI BglII Bme1580I BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EcoNI EcoO109I EcoRV FseI FspI FspAI HincII HpaI KpnI MluI MscI NaeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI