Restriction map for
pFN21AB4538
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# NO CUT ENZYMES ( 154 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BclI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TseI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 11 GACGTC GACGT^C
AcsI 595 AAATTT [GA]^AATT[CT]
AcyI 11 GACGTC G[GA]^CG[CT]C
AlwI 825 GATCC GGATC(4/5)
AlwNI 707 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 595 AAATTT [GA]^AATT[CT]
Asp700I 420 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1175 GTGCTC G[AT]GC[AT]^C
AspI 970 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 163 CCCGAG C^[CT]CG[GA]G
BciVI 35 GTATCC GTATCC(6/5)
BcnI 483 CCGGG CC^[GC]GG
BmyI 1175 GTGCTC G[AGT]GC[ACT]^C
BsaHI 11 GACGTC G[GA]^CG[CT]C
BsaI 1160 GGTCTC GGTCTC(1/5)
BseRI 1075 GAGGAG GAGGAG(10/8)
BsiHKAI 1175 GTGCTC G[AT]GC[AT]^C
BsmFI 447 GTCCC GGGAC(10/14)
BsoBI 163 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1175 GTGCTC G[AGT]GC[ACT]^C
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BstXI 828 CCACAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
DrdI 295 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 936 CGGCCA [CT]^GGCC[GA]
EarI 808 CTCTTC CTCTTC(1/4)
FauI 250 GCGGG CCCGC(4/6)
Fnu4HI 374 GCCGC GC^[ACGT]GC
Hin1I 11 GACGTC G[GA]^CG[CT]C
HphI 817 GGTGA GGTGA(8/7)
ItaI 374 GCCGC GC^[ACGT]GC
Ksp632I 808 CTCTTC CTCTTC(1/4)
NciI 483 CCGGG CC^[GC]GG
NlaIII 225 CATG CATG^
NspI 224 GCATGC [GA]CATG^[CT]
PflFI 970 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 519 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 533 GAGTC GAGTC(4/5)
SexAI 971 ACCAGGT A^CC[AT]GGT
SfaNI 371 GATGC GCATC(5/9)
SfcI 636 CTACAG C^T[GA][CT]AG
SmlI 1139 CTCAAG C^T[CT][GA]AG
SphI 224 GCATGC GCATG^C
TfiI 487 GAATC G^A[AT]TC
Tsp45I 304 GTCAC ^GT[GC]AC
Tth111I 970 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 519 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 96 CCAAGAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 420 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 375 CCGC CCGC(-3/-1)
250 GCGG
AluI 194 AGCT AG^CT
857 AGCT
AocI 297 CCTGAGG CC^T[ACGT]AGG
948 CCTGAGG
AvaII 838 GGACC G^G[AT]CC
975 GGTCC
BbsI 55 GTCTTC GAAGAC(2/6)
535 GTCTTC
BglII 804 AGATCT A^GATCT
1110 AGATCT
BpuAI 55 GTCTTC GAAGAC(2/6)
535 GTCTTC
BsaJI 164 CCGAGG C^C[ACGT][ACGT]GG
491 CCTGGG
BsrI 173 ACTGG ACTGG(1/-1)
1006 ACTGG
BstYI 804 AGATCT [GA]^GATC[CT]
1110 AGATCT
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
948 CCTGAGG
DraI 346 TTTAAA TTT^AAA
1182 TTTAAA
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
948 CCTGAGG
EcoRV 150 GATATC GAT^ATC
1069 GATATC
HinfI 487 GAATC G^A[ACGT]TC
533 GAGTC
MaeIII 304 GTCAC ^GT[ACGT]AC
316 GTTAC
MflI 804 AGATCT [GA]^GATC[CT]
1110 AGATCT
MslI 306 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
621 CATTCTTATG
ScaI 694 AGTACT AGT^ACT
863 AGTACT
TaqI 15 TCGA T^CGA
1073 TCGA
TthHB8I 15 TCGA T^CGA
1073 TCGA
XhoII 804 AGATCT [GA]^GATC[CT]
1110 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII FokI HaeIII HapII
HpaII MaeII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tru9I Tsp509I TspRI