(Circular) (Six-base) MAPSORT of: pFN21AB4538.seq Check: 4954 from: 1 to: 5896 pFN21AB4538 5896 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2020 3706 278 Size: 53 83 186 936 484 1686 2468 Fragments arranged by size: 2468 1686 936 484 186 83 53 AccI GT'mk_AC Cuts at: 1937 3239 1937 Size: 1302 4594 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 2885 3008 3046 3959 5343 5611 1632 Size: 1058 195 123 38 913 1384 268 1917 Fragments arranged by size: 1917 1384 1058 913 268 195 123 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 2315 1166 Size: 1149 4747 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5896 AlwNI CAG_nnn'CTG Cuts at: 2717 3639 5211 2717 Size: 922 1572 3402 Fragments arranged by size: 3402 1572 922 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 2600 3205 3431 3583 5692 1493 Size: 1107 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 1107 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 2168 3221 1870 Size: 73 225 1053 4545 Fragments arranged by size: 4545 1053 225 73 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3217 4672 618 Size: 325 217 627 1430 1455 1842 Fragments arranged by size: 1842 1455 1430 627 325 217 BanII G_rGCy'C Cuts at: 1391 3215 1391 Size: 1824 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2053 2533 928 Size: 971 154 480 4291 Fragments arranged by size: 4291 971 480 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2051 3788 4998 2051 Size: 1737 1210 2949 Fragments arranged by size: 2949 1737 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4507 136 Size: 107 122 71 631 605 2835 1525 Fragments arranged by size: 2835 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2809 3115 5891 2809 Size: 306 2776 2814 Fragments arranged by size: 2814 2776 306 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 3957 5113 1790 Size: 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 BmgBI CAC'GTC Cuts at: 1318 2313 1318 Size: 995 4901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 4585 442 Size: 416 53 83 399 24 2210 958 1753 Fragments arranged by size: 2210 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4556 1239 Size: 3317 2579 Bpu10I CC'TnA_GC Cuts at: 2225 2225 Size: 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3129 4021 4886 5184 3129 Size: 892 865 298 3841 Fragments arranged by size: 3841 892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3171 4559 882 Size: 617 1672 1388 2219 Fragments arranged by size: 2219 1672 1388 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2017 3703 4085 4720 Size: 53 83 186 936 484 1686 382 635 Cuts at: 4720 275 Size: 1451 Fragments arranged by size: 1686 1451 936 635 484 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4329 4727 5001 5148 5559 4329 Size: 398 274 147 411 4666 Fragments arranged by size: 4666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3089 3119 1178 Size: 30 165 30 1686 30 3955 Fragments arranged by size: 3955 1686 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3095 1186 Size: 1909 3987 BseYI C'CCAG_C Cuts at: 2548 5099 5595 2548 Size: 2551 496 2849 Fragments arranged by size: 2849 2551 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3184 3957 4042 5113 3184 Size: 773 85 1071 3967 Fragments arranged by size: 3967 1071 773 85 BsmI GAATG_Cn' Cuts at: 3407 3500 3407 Size: 93 5803 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3184 3215 3957 4042 5113 1391 Size: 399 1394 31 742 85 1071 2174 Fragments arranged by size: 2174 1394 1071 742 399 85 31 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3251 844 Size: 2407 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4391 4565 65 Size: 1183 3143 174 1396 Fragments arranged by size: 3143 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 4540 4727 1700 Size: 110 395 2335 187 2869 Fragments arranged by size: 2869 2335 395 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5896 BstXI CCAn_nnnn'nTGG Cuts at: 2840 2840 Size: 5896 BstYI r'GATC_y Cuts at: 1972 2809 3115 3226 3593 3978 3995 5447 5533 Size: 837 306 111 367 385 17 1452 86 Cuts at: 5533 5545 5891 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 837 385 367 346 306 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2303 2954 2303 Size: 651 5245 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5344 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2751 487 Size: 683 56 1525 3632 Fragments arranged by size: 3632 1525 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3412 4206 4226 2390 Size: 1022 794 20 4060 Fragments arranged by size: 4060 1022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 2353 3189 3200 2353 Size: 836 11 5049 Fragments arranged by size: 5049 836 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2306 4903 809 Size: 507 990 2597 1802 Fragments arranged by size: 2597 1802 990 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 3343 4232 Size: 54 1425 50 275 18 1111 402 889 Cuts at: 4232 5861 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1111 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3826 1353 Size: 1466 1007 3423 Fragments arranged by size: 3423 1466 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 2885 3008 3046 3959 4556 5343 Size: 393 1058 195 123 38 913 597 787 Cuts at: 5343 5611 1239 Size: 268 1524 Fragments arranged by size: 1524 1058 913 787 597 393 268 195 123 38 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 EcoRV GAT'ATC Cuts at: 2157 3076 2157 Size: 919 4977 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2645 2645 Size: 5896 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3149 3181 4537 4569 4611 Size: 32 135 32 1577 32 1356 32 42 Cuts at: 4611 4643 1373 Size: 32 2626 Fragments arranged by size: 2626 1577 1356 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1993 5010 5194 1305 Size: 688 3017 184 2007 Fragments arranged by size: 3017 2007 688 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4074 Fragments arranged by size: 4074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2630 3854 4213 4372 518 Size: 648 1149 315 1224 359 159 2042 Fragments arranged by size: 2042 1224 1149 648 359 315 159 MspA1I CmG'CkG Cuts at: 1606 3312 3989 5137 5382 1606 Size: 1706 677 1148 245 2120 Fragments arranged by size: 2120 1706 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5344 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2233 3254 4799 1132 Size: 97 1004 1021 1545 2229 Fragments arranged by size: 2229 1545 1021 1004 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 1108 Size: 1422 4474 PfoI T'CCnGG_A Cuts at: 2487 2487 Size: 5896 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2195 2227 2195 Size: 32 5864 PstI C_TGCA'G Cuts at: 830 3248 830 Size: 2418 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 2701 2870 4144 5856 2701 Size: 169 1274 1712 2741 Fragments arranged by size: 2741 1712 1274 169 SexAI A'CCwGG_T Cuts at: 2976 2976 Size: 5896 SfcI C'TryA_G Cuts at: 826 1045 2641 3244 4379 5060 5251 826 Size: 219 1596 603 1135 681 191 1471 Fragments arranged by size: 1596 1471 1135 681 603 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3144 4000 4901 5163 820 Size: 197 926 1201 856 901 262 1553 Fragments arranged by size: 1553 1201 926 901 856 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 2233 3254 1132 Size: 1101 1021 3774 Fragments arranged by size: 3774 1101 1021 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5896 StyI C'CwwG_G Cuts at: 513 1065 3335 513 Size: 552 2270 3074 Fragments arranged by size: 3074 2270 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2774 3923 3940 4093 4278 4723 325 Size: 1255 1194 1149 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 1149 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 2699 2868 Size: 274 80 33 51 1443 234 488 169 Cuts at: 2868 3127 4142 5854 96 Size: 259 1015 1712 138 Fragments arranged by size: 1712 1443 1015 488 274 259 234 169 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5856 5888 5856 Size: 32 5864 Tth111I GACn'n_nGTC Cuts at: 1316 2978 1316 Size: 1662 4234 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2108 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 2429 4025 2429 Size: 1596 4300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2018 3704 276 Size: 53 83 186 936 484 1686 2468 Fragments arranged by size: 2468 1686 936 484 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI