Restriction map for
pFN21AB6110
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BbrPI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 899 CGCG CG^CG
AccIII 881 TCCGGA T^CCGGA
AcyI 1758 GGCGCC G[GA]^CG[CT]C
AflIII 1093 ACATGT A^C[GA][CT]GT
Alw44I 367 GTGCAC G^TGCAC
AlwNI 752 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1835 CCTCAGG CC^T[ACGT]AGG
ApaLI 367 GTGCAC G^TGCAC
BamHI 1207 GGATCC G^GATCC
BbeI 1758 GGCGCC GGCGC^C
BbsI 201 GAAGAC GAAGAC(2/6)
BbvCI 1412 CCTCAGC CCTCAGC(-5/-2)
BciVI 878 GTATCC GTATCC(6/5)
BclI 908 TGATCA T^GATCA
BpuAI 201 GAAGAC GAAGAC(2/6)
BsaBI 162 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1758 GGCGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsaWI 881 TCCGGA [AT]^CCGG[AT]
BseAI 881 TCCGGA T^CCGGA
BseRI 1372 GAGGAG GAGGAG(10/8)
BspEI 881 TCCGGA T^CCGGA
BspLU11I 1093 ACATGT A^CATGT
BsrDI 392 CATTGC GCAATG(2/0)
BssSI 103 CACGAG CACGAG(-5/-1)
BstEII 670 GGTTACC G^GT[ACGT]ACC
BstPI 670 GGTTACC G^GT[ACGT]ACC
BstUI 899 CGCG CG^CG
Bsu36I 1835 CCTCAGG CC^T[ACGT]AGG
DraIII 25 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1835 CCTCAGG CC^T[ACGT]AGG
EcoNI 111 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 670 GGTTACC G^GT[ACGT]ACC
FbaI 908 TGATCA T^GATCA
HgaI 188 GACGC GACGC(5/10)
Hin1I 1758 GGCGCC G[GA]^CG[CT]C
HincII 837 GTTGAC GT[CT]^[GA]AC
HindII 837 GTTGAC GT[CT]^[GA]AC
KasI 1758 GGCGCC G^GCGCC
MaeII 1504 ACGT A^CGT
MamI 162 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 780 CAATTG C^AATTG
MroI 881 TCCGGA T^CCGGA
MseI 19 TTAA T^TAA
MunI 780 CAATTG C^AATTG
MvnI 899 CGCG CG^CG
NarI 1758 GGCGCC GG^CGCC
SexAI 824 ACCTGGT A^CC[AT]GGT
SfoI 1758 GGCGCC GGC^GCC
SmlI 1806 CTTGAG C^T[CT][GA]AG
Tru9I 19 TTAA T^TAA
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# TWO CUT ENZYMES ( 8 enzymes) #
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AhdI 1608 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1851 GACTGGCTGTC
AspEI 1608 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1851 GACTGGCTGTC
BpmI 55 CTCCAG CTGGAG(16/14)
1282 CTCCAG
Eam1105I 1608 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1851 GACTGGCTGTC
HaeII 1747 AGCGCC [GA]GCGC^[CT]
1758 GGCGCC
NspI 341 ACATGC [GA]CATG^[CT]
1093 ACATGT
PflMI 1191 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCAGGGTATGG
Van91I 1191 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCAGGGTATGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
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AciI AfaI AluI AlwI ApaI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII