(Circular) (Six-base) MAPSORT of: pFN21AB6110.seq Check: 3696 from: 1 to: 6589 pFN21AB6110 6589 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3038 3048 3565 3038 Size: 10 517 6062 Fragments arranged by size: 6062 517 10 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3593 4652 6036 6304 1632 Size: 527 1434 1059 1384 268 1917 Fragments arranged by size: 1917 1434 1384 1059 527 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 3098 5488 6465 1203 Size: 1895 2390 977 1327 Fragments arranged by size: 2390 1895 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 3618 3861 5318 3618 Size: 243 1457 4889 Fragments arranged by size: 4889 1457 243 AleI CACnn'nnGTG Cuts at: 1166 2777 2980 1166 Size: 1611 203 4775 Fragments arranged by size: 4775 1611 203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2862 1184 Size: 1678 4911 AlwNI CAG_nnn'CTG Cuts at: 2762 4332 5904 2762 Size: 1570 1572 3447 Fragments arranged by size: 3447 1572 1570 ApaI G_GGCC'C Cuts at: 2220 2330 3719 2220 Size: 110 1389 5090 Fragments arranged by size: 5090 1389 110 ApaLI G'TGCA_C Cuts at: 2372 4646 5802 2372 Size: 2274 1156 3159 Fragments arranged by size: 3159 2274 1156 ApoI r'AATT_y Cuts at: 1493 3898 4124 4276 6385 1493 Size: 2405 226 152 2109 1697 Fragments arranged by size: 2405 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 3914 1870 Size: 73 1971 4545 Fragments arranged by size: 4545 1971 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3533 3566 3533 Size: 33 6556 BamHI G'GATC_C Cuts at: 3212 3919 4286 3212 Size: 707 367 5515 Fragments arranged by size: 5515 707 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2315 3219 3763 3910 Size: 325 217 627 465 63 904 544 147 Cuts at: 3910 5365 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 904 627 544 465 325 217 147 63 BanII G_rGCy'C Cuts at: 1391 2061 2115 2220 2330 2427 2709 3497 3719 Size: 670 54 105 110 97 282 788 222 Cuts at: 3719 3743 3908 1391 Size: 24 165 4072 Fragments arranged by size: 4072 788 670 282 222 165 110 105 97 54 24 BbeI G_GCGC'C Cuts at: 3767 3767 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2213 928 Size: 971 314 5304 Fragments arranged by size: 5304 971 314 BbvCI CC'TCA_GC Cuts at: 3418 3418 Size: 6589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2894 4481 5691 2894 Size: 1587 1210 3792 Fragments arranged by size: 3792 1587 1210 BclI T'GATC_A Cuts at: 1548 2913 1548 Size: 1365 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2713 3431 3761 Size: 107 122 71 631 605 1041 718 330 Cuts at: 3761 5200 136 Size: 1439 1525 Fragments arranged by size: 1525 1439 1041 718 631 605 330 122 107 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 2155 2220 2330 2376 2666 2911 2987 3025 Size: 365 65 110 46 290 245 76 38 Cuts at: 3025 3224 3509 3719 4650 5806 1790 Size: 199 285 210 931 1156 2573 Fragments arranged by size: 2573 1156 931 365 290 285 245 210 199 110 76 65 46 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3293 3578 4320 Size: 416 53 83 399 24 1876 285 742 Cuts at: 4320 5278 442 Size: 958 1753 Fragments arranged by size: 1876 1753 958 742 416 399 285 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 3272 5249 1239 Size: 806 1227 1977 2579 Fragments arranged by size: 2579 1977 1227 806 Bpu10I CC'TnA_GC Cuts at: 2579 3418 3488 2579 Size: 839 70 5680 Fragments arranged by size: 5680 839 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4714 5579 5877 3832 Size: 882 865 298 4544 Fragments arranged by size: 4544 882 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 5252 882 Size: 617 1092 2661 2219 Fragments arranged by size: 2661 2219 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 4380 493 Size: 711 3176 2702 Fragments arranged by size: 3176 2702 711 BsaBI GATnn'nnATC Cuts at: 2171 6583 2171 Size: 4412 2177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3764 4396 4778 5413 Size: 53 83 186 936 2231 632 382 635 Cuts at: 5413 275 Size: 1451 Fragments arranged by size: 2231 1451 936 635 632 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2886 5022 5420 5694 5841 6252 2886 Size: 2136 398 274 147 411 3223 Fragments arranged by size: 3223 2136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6364 Fragments arranged by size: 6364 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3392 1186 Size: 2206 4383 BseYI C'CCAG_C Cuts at: 2014 2323 2364 2502 2717 3733 5792 6288 2014 Size: 309 41 138 215 1016 2059 496 2315 Fragments arranged by size: 2315 2059 1016 496 309 215 138 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 2531 2594 1163 Size: 573 378 417 63 5158 Fragments arranged by size: 5158 573 417 378 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4039 4800 5828 1540 Size: 275 186 2038 761 1028 2301 Fragments arranged by size: 2301 2038 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2115 2376 2427 3743 4650 4735 5806 2061 Size: 54 261 51 1316 907 85 1071 2844 Fragments arranged by size: 2844 1316 1071 907 261 85 54 51 BsmI GAATG_Cn' Cuts at: 4100 4193 4100 Size: 93 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2115 2155 2220 2330 2376 2427 Size: 399 271 54 40 65 110 46 51 Cuts at: 2427 2666 2709 2911 2987 3025 3224 3497 3509 Size: 239 43 202 76 38 199 273 12 Cuts at: 3509 3719 3743 3908 4650 4735 5806 1391 Size: 210 24 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 273 271 239 210 202 199 165 110 85 76 65 54 51 46 43 40 38 24 12 BspEI T'CCGG_A Cuts at: 2886 6252 2886 Size: 3366 3223 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 3048 3565 3944 844 Size: 2194 10 517 379 3489 Fragments arranged by size: 3489 2194 517 379 10 BsrBI CCG'CTC Cuts at: 1394 1823 4476 1394 Size: 429 2653 3507 Fragments arranged by size: 3507 2653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 5084 5258 65 Size: 1183 1148 2688 174 1396 Fragments arranged by size: 2688 1396 1183 1148 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 2108 4649 5661 2108 Size: 2541 1012 3036 Fragments arranged by size: 3036 2541 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6589 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 2675 1184 Size: 1491 5098 BstYI r'GATC_y Cuts at: 1972 3192 3212 3313 3382 3919 4286 4671 4688 Size: 1220 20 101 69 537 367 385 17 Cuts at: 4688 6140 6226 6238 6584 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1220 537 385 367 346 101 86 69 20 17 12 Bsu36I CC'TnA_GG Cuts at: 3841 3841 Size: 6589 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 3745 4105 4899 4919 3745 Size: 360 794 20 5415 Fragments arranged by size: 5415 794 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2425 2460 529 Size: 35 1861 35 4658 Fragments arranged by size: 4658 1861 35 35 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DraIII CAC_nnn'GTG Cuts at: 2035 2035 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2118 2952 Size: 54 1425 50 275 18 253 35 834 Cuts at: 2952 3466 3673 3775 4036 4925 6554 8 Size: 514 207 102 261 889 1629 43 Fragments arranged by size: 1629 1425 889 834 514 275 261 253 207 102 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 4036 1537 Size: 275 2224 4090 Fragments arranged by size: 4090 2224 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5176 5550 5696 387 Size: 1064 138 435 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2113 2425 3741 2059 Size: 54 312 1316 4907 Fragments arranged by size: 4907 1316 312 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2159 3272 3593 4652 5249 6036 Size: 393 413 114 1113 321 1059 597 787 Cuts at: 6036 6304 1239 Size: 268 1524 Fragments arranged by size: 1524 1113 1059 787 597 413 393 321 268 114 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 6589 EcoO109I rG'GnC_Cy Cuts at: 2216 2217 2327 3696 3807 3830 3845 2216 Size: 1 110 1369 111 23 15 4960 Fragments arranged by size: 4960 1369 111 110 23 15 1 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 3756 3767 5736 1678 Size: 2078 11 1969 2531 Fragments arranged by size: 2531 2078 1969 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3512 3544 3564 3596 3604 Size: 32 135 32 1940 32 20 32 8 Cuts at: 3604 3636 3688 3720 3847 3879 5230 5262 5304 Size: 32 52 32 127 32 1351 32 42 Cuts at: 5304 5336 1373 Size: 32 2626 Fragments arranged by size: 2626 1940 1351 135 127 52 42 32 32 32 32 32 32 32 32 32 20 8 HincII GTy'rAC Cuts at: 669 1657 1938 2844 3933 4179 669 Size: 988 281 906 1089 246 3079 Fragments arranged by size: 3079 1089 988 906 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 3763 3763 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 2785 4188 2785 Size: 1403 5186 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5703 5887 1305 Size: 4398 184 2007 Fragments arranged by size: 4398 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 2954 3468 3675 10 Size: 54 1425 343 288 834 514 207 2924 Fragments arranged by size: 2924 1425 834 514 343 288 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2379 2570 2777 2980 3097 4547 Size: 648 870 343 191 207 203 117 1450 Cuts at: 4547 4906 5065 518 Size: 359 159 2042 Fragments arranged by size: 2042 1450 870 648 359 343 207 203 191 159 117 MspA1I CmG'CkG Cuts at: 1606 2148 2464 2604 3422 4005 4682 5830 6075 Size: 542 316 140 818 583 677 1148 245 Cuts at: 6075 1606 Size: 2120 Fragments arranged by size: 2120 1148 818 677 583 542 316 245 140 NarI GG'CG_CC Cuts at: 3764 3764 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 2350 3102 3947 5492 1132 Size: 97 1121 752 845 1545 2229 Fragments arranged by size: 2229 1545 1121 845 752 97 PasI CC'CwG_GG Cuts at: 1348 2212 3800 1348 Size: 864 1588 4137 Fragments arranged by size: 4137 1588 864 PciI A'CATG_T Cuts at: 3098 5488 3098 Size: 2390 4199 PflMI CCAn_nnn'nTGG Cuts at: 1108 3202 3272 1108 Size: 2094 70 4425 Fragments arranged by size: 4425 2094 70 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3696 3830 3845 3696 Size: 134 15 6440 Fragments arranged by size: 6440 134 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PspOMI G'GGCC_C Cuts at: 2216 2326 3715 2216 Size: 110 1389 5090 Fragments arranged by size: 5090 1389 110 PstI C_TGCA'G Cuts at: 830 2851 3045 3752 3941 830 Size: 2021 194 707 189 3478 Fragments arranged by size: 3478 2021 707 194 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2148 2604 3422 2148 Size: 456 818 5315 Fragments arranged by size: 5315 818 456 SacI G_AGCT'C Cuts at: 2061 2115 2427 3743 2061 Size: 54 312 1316 4907 Fragments arranged by size: 4907 1316 312 54 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SanDI GG'GwC_CC Cuts at: 3696 3696 Size: 6589 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SexAI A'CCwGG_T Cuts at: 2829 2829 Size: 6589 SfcI C'TryA_G Cuts at: 826 1045 2262 2847 3041 3068 3748 3937 5072 Size: 219 1217 585 194 27 680 189 1135 Cuts at: 5072 5753 5944 826 Size: 681 191 1471 Fragments arranged by size: 1471 1217 1135 681 680 585 219 194 191 189 27 SfoI GGC'GCC Cuts at: 3765 3765 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4693 5594 5856 820 Size: 197 926 1868 882 901 262 1553 Fragments arranged by size: 1868 1553 926 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 3583 3670 4028 513 Size: 552 939 117 1462 87 358 3074 Fragments arranged by size: 3074 1462 939 552 358 117 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3855 4616 4633 4786 4971 5416 Size: 1255 1923 352 761 17 153 185 445 Cuts at: 5416 325 Size: 1498 Fragments arranged by size: 1923 1498 1255 761 445 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3291 4835 6547 Size: 274 80 33 51 1443 1314 1544 1712 Cuts at: 6547 96 Size: 138 Fragments arranged by size: 1712 1544 1443 1314 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 3461 3493 3546 3578 6549 6581 2080 Size: 32 1349 32 53 32 2971 32 2088 Fragments arranged by size: 2971 2088 1349 53 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2069 2338 2776 2011 Size: 58 269 438 5824 Fragments arranged by size: 5824 438 269 58 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI