################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwI 123 GGATC GGATC(4/5) AocI 378 CCTCAGG CC^T[ACGT]AGG ApaI 224 GGGCCC GGGCC^C BanII 224 GGGCCC G[GA]GC[CT]^C BcgI 501 GCAGGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 389 CTAG C^TAG BglI 375 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 224 GGGCCC G[AGT]GC[ACT]^C Bsp1286I 224 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 708 TGTACA T^GTACA BsrFI 433 GCCGGC [GA]^CCGG[CT] BsrGI 708 TGTACA T^GTACA BsrI 713 ACTGG ACTGG(1/-1) BssSI 680 CACGAG CACGAG(-5/-1) Bsu36I 378 CCTCAGG CC^T[ACGT]AGG Cfr10I 433 GCCGGC [GA]^CCGG[CT] EaeI 431 TGGCCG [CT]^GGCC[GA] EarI 138 GAAGAG CTCTTC(1/4) Eco81I 378 CCTCAGG CC^T[ACGT]AGG EcoT14I 218 CCTTGG C^C[AT][AT]GG FauI 92 CCCGC CCCGC(4/6) HapII 434 CCGG C^CGG HindIII 702 AAGCTT A^AGCTT HpaII 434 CCGG C^CGG Ksp632I 138 GAAGAG CTCTTC(1/4) MaeI 389 CTAG C^TAG MspI 434 CCGG C^CGG NaeI 433 GCCGGC GCC^GGC NgoMIV 433 GCCGGC G^CCGGC PleI 604 GACTC GAGTC(4/5) PpuMI 569 GGGACCC [GA]G^G[AT]CC[CT] PstI 334 CTGCAG CTGCA^G SfaNI 49 GATGC GCATC(5/9) SfcI 334 CTGCAG C^T[GA][CT]AG SmlI 699 CTCAAG C^T[CT][GA]AG SphI 671 GCATGC GCATG^C SspBI 708 TGTACA T^GTACA StyI 218 CCTTGG C^C[AT][AT]GG TaqI 510 TCGA T^CGA TfiI 520 GAATC G^A[AT]TC Tsp45I 214 GTCAC ^GT[GC]AC TthHB8I 510 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 548 GTAC GT^AC 709 GTAC AvaII 359 GGACC G^G[AT]CC 570 GGACC BpmI 340 CTGGAG CTGGAG(16/14) 608 CTGGAG BstF5I 282 CATCC GGATG(2/0) 289 CATCC DdeI 305 CTAAG C^T[ACGT]AG 379 CTCAG FokI 282 CATCC GGATG(9/13) 289 CATCC HinfI 520 GAATC G^A[ACGT]TC 604 GACTC MaeIII 214 GTCAC ^GT[ACGT]AC 626 GTAAC MseI 581 TTAA T^TAA 596 TTAA MspA1I 182 CAGCTG C[AC]G^C[GT]G 337 CAGCTG NspI 421 ACATGC [GA]CATG^[CT] 671 GCATGC PvuII 182 CAGCTG CAG^CTG 337 CAGCTG RsaI 548 GTAC GT^AC 709 GTAC Tru9I 581 TTAA T^TAA 596 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BbvI BsaJI BsgI BsiYI BslI BsmAI BsmFI BssKI BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI