(Linear) (Six-base) MAPSORT of: KIEE1494.seq Check: 4054 from: 1 to: 741 KIEE1494.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 741 Size: 157 584 ApaI G_GGCC'C Cuts at: 0 228 741 Size: 228 513 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 531 564 741 Size: 531 33 177 Fragments arranged by size: 531 177 33 BanII G_rGCy'C Cuts at: 0 228 741 Size: 228 513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 490 524 741 Size: 490 34 217 Fragments arranged by size: 490 217 34 BglI GCCn_nnn'nGGC Cuts at: 0 381 741 Size: 381 360 Bme1580I G_kGCm'C Cuts at: 0 228 741 Size: 228 513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 629 741 Size: 361 268 112 Fragments arranged by size: 361 268 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 684 741 Size: 684 57 BseYI C'CCAG_C Cuts at: 0 254 437 741 Size: 254 183 304 Fragments arranged by size: 304 254 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 520 672 741 Size: 391 129 152 69 Fragments arranged by size: 391 152 129 69 Bsp1286I G_dGCh'C Cuts at: 0 228 741 Size: 228 513 BsrFI r'CCGG_y Cuts at: 0 433 741 Size: 433 308 BsrGI T'GTAC_A Cuts at: 0 708 741 Size: 708 33 BssSI C'ACGA_G Cuts at: 0 680 741 Size: 680 61 Bsu36I CC'TnA_GG Cuts at: 0 379 741 Size: 379 362 BtsI GCAGTG_nn' Cuts at: 0 432 741 Size: 432 309 EaeI y'GGCC_r Cuts at: 0 431 741 Size: 431 310 EarI CTCTTCn'nnn_ Cuts at: 0 133 741 Size: 133 608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 361 629 741 Size: 157 204 268 112 Fragments arranged by size: 268 204 157 112 EcoO109I rG'GnC_Cy Cuts at: 0 224 570 613 741 Size: 224 346 43 128 Fragments arranged by size: 346 224 128 43 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 609 741 Size: 609 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 108 115 140 147 171 203 741 Size: 108 7 25 7 24 32 538 Fragments arranged by size: 538 108 32 25 24 7 7 HindIII A'AGCT_T Cuts at: 0 702 741 Size: 702 39 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 341 741 Size: 341 400 MspA1I CmG'CkG Cuts at: 0 184 339 741 Size: 184 155 402 Fragments arranged by size: 402 184 155 NaeI GCC'GGC Cuts at: 0 435 741 Size: 435 306 NgoMIV G'CCGG_C Cuts at: 0 433 741 Size: 433 308 NspI r_CATG'y Cuts at: 0 425 675 741 Size: 425 250 66 Fragments arranged by size: 425 250 66 PasI CC'CwG_GG Cuts at: 0 41 456 639 741 Size: 41 415 183 102 Fragments arranged by size: 415 183 102 41 PpuMI rG'GwC_Cy Cuts at: 0 570 741 Size: 570 171 PspOMI G'GGCC_C Cuts at: 0 224 741 Size: 224 517 PstI C_TGCA'G Cuts at: 0 338 741 Size: 338 403 PvuII CAG'CTG Cuts at: 0 184 339 741 Size: 184 155 402 Fragments arranged by size: 402 184 155 SanDI GG'GwC_CC Cuts at: 0 570 741 Size: 570 171 SfcI C'TryA_G Cuts at: 0 334 741 Size: 334 407 SmlI C'TyrA_G Cuts at: 0 699 741 Size: 699 42 SphI G_CATG'C Cuts at: 0 675 741 Size: 675 66 StyI C'CwwG_G Cuts at: 0 218 741 Size: 218 523 TatI w'GTAC_w Cuts at: 0 708 741 Size: 708 33 Enzymes that do cut: AcuI ApaI BaeI BanII BcgI BglI Bme1580I BpmI BpuEI BseYI BsgI Bsp1286I BsrFI BsrGI BssSI Bsu36I BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I HindIII MmeI MspA1I NaeI NgoMIV NspI PasI PpuMI PspOMI PstI PvuII SanDI SfcI SmlI SphI StyI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI