################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbeI BbrPI BciVI BclI BglI BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1166 GGTACC G^GTACC AccI 820 GTCGAC GT^[AC][GT]AC AflII 814 CTTAAG C^TTAAG AocI 438 CCTGAGG CC^T[ACGT]AGG Asp718I 1166 GGTACC G^GTACC AspI 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 842 CCCGAG C^[CT]CG[GA]G BalI 1753 TGGCCA TGG^CCA BanI 1166 GGTACC G^G[CT][GA]CC BbvCI 925 GCTGAGG CCTCAGC(-5/-2) BcgI 822 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 235 CTAG C^TAG BfrI 814 CTTAAG C^TTAAG BsaI 25 GAGACC GGTCTC(1/5) BsaWI 1748 TCCGGT [AT]^CCGG[AT] BsgI 858 GTGCAG GTGCAG(16/14) BsmBI 1723 CGTCTC CGTCTC(1/5) BsoBI 842 CCCGAG C^[CT]CG[GA]G BspHI 1898 TCATGA T^CATGA BsrBI 700 GAGCGG CCGCTC(-3/-3) BstAPI 271 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1654 GGTTACC G^GT[ACGT]ACC BstPI 1654 GGTTACC G^GT[ACGT]ACC Bsu36I 438 CCTGAGG CC^T[ACGT]AGG DrdI 2047 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1753 TGGCCA [CT]^GGCC[GA] Eco81I 438 CCTGAGG CC^T[ACGT]AGG EcoO65I 1654 GGTTACC G^GT[ACGT]ACC EcoRI 547 GAATTC G^AATTC KpnI 1166 GGTACC GGTAC^C MaeI 235 CTAG C^TAG MluNI 1753 TGGCCA TGG^CCA MscI 1753 TGGCCA TGG^CCA NspI 1706 GCATGT [GA]CATG^[CT] PflFI 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 777 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 52 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1898 TCATGA T^CATGA SalI 820 GTCGAC G^TCGAC SapI 576 GAAGAGC GCTCTTC(1/4) SexAI 1910 ACCAGGT A^CC[AT]GGT TaqI 821 TCGA T^CGA Tth111I 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 821 TCGA T^CGA Van91I 777 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 547 GAATTC [GA]^AATT[CT] 1447 AAATTT ApaI 737 GGGCCC GGGCC^C 1104 GGGCCC ApoI 547 GAATTC [GA]^AATT[CT] 1447 AAATTT BcnI 882 CCGGG CC^[GC]GG 1520 CCGGG BglII 1328 AGATCT A^GATCT 1820 AGATCT BsmI 1890 GAATGC GAATGC(1/-1) 1495 GCATTC BsrDI 1305 CATTGC GCAATG(2/0) 1503 CATTGC EcoNI 810 CCTGCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1263 CCTCCAGAAGG HgaI 268 GACGC GACGC(5/10) 392 GCGTC HincII 820 GTCGAC GT[CT]^[GA]AC 1203 GTTGAC HindII 820 GTCGAC GT[CT]^[GA]AC 1203 GTTGAC MaeII 164 ACGT A^CGT 220 ACGT NciI 882 CCGGG CC^[GC]GG 1520 CCGGG NdeI 175 CATATG CA^TATG 1757 CATATG PleI 331 GAGTC GAGTC(4/5) 918 GAGTC PpuMI 956 GGGACCT [GA]G^G[AT]CC[CT] 1550 GGGACCT SmlI 814 CTTAAG C^T[CT][GA]AG 2011 CTCAAG XcmI 584 CCAGAGAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1502 CCATTGCAGTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbsI BbvI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII