(Circular) (Six-base) MAPSORT of: pFN21AE6726.seq Check: 423 from: 1 to: 6799 pFN21AE6726 6799 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4609 278 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2826 4142 1937 Size: 889 1316 4594 Fragments arranged by size: 4594 1316 889 Acc65I G'GTAC_C Cuts at: 3171 4120 3171 Size: 949 5850 AclI AA'CG_TT Cuts at: 1563 4926 5299 1563 Size: 3363 373 3063 Fragments arranged by size: 3363 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 2348 2531 2558 2678 2880 3347 4862 Size: 585 131 183 27 120 202 467 1515 Cuts at: 4862 6246 6514 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1515 1384 585 467 268 202 183 131 120 27 AflII C'TTAA_G Cuts at: 820 1017 2819 820 Size: 197 1802 4800 Fragments arranged by size: 4800 1802 197 AflIII A'CryG_T Cuts at: 1203 5698 6675 1203 Size: 4495 977 1327 Fragments arranged by size: 4495 1327 977 AgeI A'CCGG_T Cuts at: 5630 5630 Size: 6799 AhdI GACnn_n'nnGTC Cuts at: 5528 5528 Size: 6799 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2808 1184 Size: 1624 5175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3357 3389 3357 Size: 32 6767 AlwNI CAG_nnn'CTG Cuts at: 2788 3000 3181 3421 4542 6114 2788 Size: 212 181 240 1121 1572 3473 Fragments arranged by size: 3473 1572 1121 240 212 181 ApaI G_GGCC'C Cuts at: 2746 3113 2746 Size: 367 6432 ApaLI G'TGCA_C Cuts at: 4856 6012 4856 Size: 1156 5643 ApoI r'AATT_y Cuts at: 1493 2552 3452 4108 4334 4486 6595 1493 Size: 1059 900 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 900 656 226 152 AseI AT'TA_AT Cuts at: 160 1032 5353 160 Size: 872 4321 1606 Fragments arranged by size: 4321 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 AvaI C'yCGr_G Cuts at: 1870 1943 2847 4124 1870 Size: 73 904 1277 4545 Fragments arranged by size: 4545 1277 904 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2351 2384 2351 Size: 33 6766 BamHI G'GATC_C Cuts at: 4129 4496 4129 Size: 367 6432 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3171 4120 5575 618 Size: 325 217 627 1384 949 1455 1842 Fragments arranged by size: 1842 1455 1384 949 627 325 217 BanII G_rGCy'C Cuts at: 1391 2588 2746 2947 3093 3113 3540 3843 4004 Size: 1197 158 201 146 20 427 303 161 Cuts at: 4004 4118 1391 Size: 114 4072 Fragments arranged by size: 4072 1197 427 303 201 161 158 146 114 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 3012 3138 3645 928 Size: 971 498 615 126 507 4082 Fragments arranged by size: 4082 971 615 507 498 126 BbvCI CC'TCA_GC Cuts at: 2931 2931 Size: 6799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2816 2850 4990 5024 1903 Size: 34 50 34 795 34 2140 34 3678 Fragments arranged by size: 3678 2140 795 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4691 5901 4691 Size: 1210 5589 BclI T'GATC_A Cuts at: 1548 1548 Size: 6799 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5410 136 Size: 107 122 71 631 605 3738 1525 Fragments arranged by size: 3738 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3333 3825 6794 3333 Size: 492 2969 3338 Fragments arranged by size: 3338 2969 492 BlpI GC'TnA_GC Cuts at: 2625 2777 2937 4216 2625 Size: 152 160 1279 5208 Fragments arranged by size: 5208 1279 160 152 Bme1580I G_kGCm'C Cuts at: 1790 2746 3113 4860 6016 1790 Size: 956 367 1747 1156 2573 Fragments arranged by size: 2573 1747 1156 956 367 BmgBI CAC'GTC Cuts at: 1318 2170 1318 Size: 852 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3284 4530 5488 Size: 416 53 83 399 24 1867 1246 958 Cuts at: 5488 442 Size: 1753 Fragments arranged by size: 1867 1753 1246 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6799 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 6799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2572 3122 3254 3902 5459 1239 Size: 1333 550 132 648 1557 2579 Fragments arranged by size: 2579 1557 1333 648 550 132 Bpu10I CC'TnA_GC Cuts at: 2931 3988 2931 Size: 1057 5742 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4001 4924 5789 6087 4001 Size: 923 865 298 4713 Fragments arranged by size: 4713 923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 5462 882 Size: 617 525 3438 2219 Fragments arranged by size: 3438 2219 617 525 BsaAI yAC'GTr Cuts at: 493 1204 4590 493 Size: 711 3386 2702 Fragments arranged by size: 3386 2702 711 BsaBI GATnn'nnATC Cuts at: 6793 6793 Size: 6799 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4606 4988 5623 275 Size: 53 83 186 936 3073 382 635 1451 Fragments arranged by size: 3073 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3753 5232 5630 5904 6051 6462 3753 Size: 1479 398 274 147 411 4090 Fragments arranged by size: 4090 1479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3830 3860 1178 Size: 30 165 30 2427 30 4117 Fragments arranged by size: 4117 2427 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 2777 3028 3485 3857 1186 Size: 1288 303 251 457 372 4128 Fragments arranged by size: 4128 1288 457 372 303 251 BseYI C'CCAG_C Cuts at: 2025 2293 2340 3094 3101 6002 6498 2025 Size: 268 47 754 7 2901 496 2326 Fragments arranged by size: 2901 2326 754 496 268 47 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2884 1163 Size: 573 1148 5078 Fragments arranged by size: 5078 1148 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4249 5010 6038 1540 Size: 275 186 2248 761 1028 2301 Fragments arranged by size: 2301 2248 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2588 2631 2947 3540 4860 4945 6016 2169 Size: 419 43 316 593 1320 85 1071 2952 Fragments arranged by size: 2952 1320 1071 593 419 316 85 43 BsmI GAATG_Cn' Cuts at: 3500 3901 4310 4403 3500 Size: 401 409 93 5896 Fragments arranged by size: 5896 409 401 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3734 3734 Size: 6799 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2588 2631 2746 2947 3093 3113 Size: 399 379 419 43 115 201 146 20 Cuts at: 3113 3540 3843 4004 4118 4860 4945 6016 1391 Size: 427 303 161 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 419 399 379 303 201 161 146 115 114 85 43 20 BspEI T'CCGG_A Cuts at: 6462 6462 Size: 6799 BspHI T'CATG_A Cuts at: 3903 4688 6418 3903 Size: 785 1730 4284 Fragments arranged by size: 4284 1730 785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4154 844 Size: 3310 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2707 4686 1394 Size: 429 884 1979 3507 Fragments arranged by size: 3507 1979 884 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3309 3507 5294 5468 65 Size: 1183 2061 198 1787 174 1396 Fragments arranged by size: 2061 1787 1396 1183 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5443 5630 1700 Size: 110 3633 187 2869 Fragments arranged by size: 3633 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6799 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6799 BssSI C'ACGA_G Cuts at: 4859 5871 4859 Size: 1012 5787 BstAPI GCAn_nnn'nTGC Cuts at: 794 2282 794 Size: 1488 5311 BstBI TT'CG_AA Cuts at: 5603 5603 Size: 6799 BstEII G'GTnAC_C Cuts at: 1184 3659 1184 Size: 2475 4324 BstYI r'GATC_y Cuts at: 1972 2730 3333 3345 3528 3825 4129 4496 4881 Size: 758 603 12 183 297 304 367 385 Cuts at: 4881 4898 6350 6436 6448 6794 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 758 603 385 367 346 304 297 183 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2444 2444 Size: 6799 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6247 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6060 Fragments arranged by size: 6060 683 56 BtsI GCAGTG_nn' Cuts at: 3173 3206 4315 5109 5129 3173 Size: 33 1109 794 20 4843 Fragments arranged by size: 4843 1109 794 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6799 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6764 DraI TTT'AAA Cuts at: 4103 4103 Size: 6799 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4058 5806 809 Size: 507 2742 1748 1802 Fragments arranged by size: 2742 1802 1748 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3758 4246 5135 Size: 54 1425 50 275 18 1928 488 889 Cuts at: 5135 6764 8 Size: 1629 43 Fragments arranged by size: 1928 1629 1425 889 488 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4246 1537 Size: 275 2434 4090 Fragments arranged by size: 4090 2434 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2576 2899 3024 4729 1353 Size: 780 443 323 125 1705 3423 Fragments arranged by size: 3423 1705 780 443 323 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 5386 5760 5906 387 Size: 1064 138 1134 2663 374 146 1280 Fragments arranged by size: 2663 1280 1134 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2586 2945 3538 2586 Size: 359 593 5847 Fragments arranged by size: 5847 593 359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2348 2531 2558 2572 2678 2880 Size: 393 585 131 183 27 14 106 202 Cuts at: 2880 3122 3254 3347 3902 4862 5459 6246 6514 Size: 242 132 93 555 960 597 787 268 Cuts at: 6514 1239 Size: 1524 Fragments arranged by size: 1524 960 787 597 585 555 393 268 242 202 183 132 131 106 93 27 14 EcoNI CCTnn'n_nnAGG Cuts at: 2819 3272 2819 Size: 453 6346 EcoO109I rG'GnC_Cy Cuts at: 2041 2440 2743 2962 3109 3220 3556 2041 Size: 399 303 219 147 111 336 5284 Fragments arranged by size: 5284 399 336 303 219 147 111 EcoRI G'AATT_C Cuts at: 2552 4108 4486 2552 Size: 1556 378 4865 Fragments arranged by size: 4865 1556 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2084 2834 3352 4042 2084 Size: 750 518 690 4841 Fragments arranged by size: 4841 750 690 518 FspI TGC'GCA Cuts at: 5305 5305 Size: 6799 HaeII r_GCGC'y Cuts at: 1678 3391 3814 4096 5946 1678 Size: 1713 423 282 1850 2531 Fragments arranged by size: 2531 1850 1713 423 282 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2264 2266 2296 2483 Size: 32 135 32 662 30 2 30 187 Cuts at: 2483 2515 2893 2925 3712 3744 3772 3804 3830 Size: 32 378 32 787 32 28 32 26 Cuts at: 3830 3862 5440 5472 5514 5546 1373 Size: 32 1578 32 42 32 2626 Fragments arranged by size: 2626 1578 787 662 378 187 135 42 32 32 32 32 32 32 32 32 32 30 30 28 26 2 HincII GTy'rAC Cuts at: 669 1657 1938 2827 3210 4143 4389 669 Size: 988 281 889 383 933 246 3079 Fragments arranged by size: 3079 988 933 889 383 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6799 HpaI GTT'AAC Cuts at: 4389 4389 Size: 6799 KpnI G_GTAC'C Cuts at: 3175 4124 3175 Size: 949 5850 MfeI C'AATT_G Cuts at: 4398 4398 Size: 6799 MluI A'CGCG_T Cuts at: 6675 6675 Size: 6799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 5913 6097 1305 Size: 774 3834 184 2007 Fragments arranged by size: 3834 2007 774 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3760 10 Size: 54 1425 343 1928 3049 Fragments arranged by size: 3049 1928 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3161 3290 3789 4757 5116 5275 518 Size: 648 1995 129 499 968 359 159 2042 Fragments arranged by size: 2042 1995 968 648 499 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2788 2860 3181 4215 4892 6040 6285 1606 Size: 1182 72 321 1034 677 1148 245 2120 Fragments arranged by size: 2120 1182 1148 1034 677 321 245 72 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6247 NdeI CA'TA_TG Cuts at: 387 2181 3763 387 Size: 1794 1582 3423 Fragments arranged by size: 3423 1794 1582 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6799 NotI GC'GGCC_GC Cuts at: 4246 4246 Size: 6799 NspI r_CATG'y Cuts at: 1132 1229 3715 4157 5702 1132 Size: 97 2486 442 1545 2229 Fragments arranged by size: 2486 2229 1545 442 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6799 PciI A'CATG_T Cuts at: 5698 5698 Size: 6799 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 1108 Size: 1680 5119 PfoI T'CCnGG_A Cuts at: 2556 3524 3879 2556 Size: 968 355 5476 Fragments arranged by size: 5476 968 355 PmeI GTTT'AAAC Cuts at: 4103 4103 Size: 6799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4868 4900 6381 6413 4868 Size: 32 1481 32 5254 Fragments arranged by size: 5254 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2962 3556 2962 Size: 594 6205 PshAI GACnn'nnGTC Cuts at: 1613 2061 1613 Size: 448 6351 PsiI TTA'TAA Cuts at: 4369 4369 Size: 6799 PspOMI G'GGCC_C Cuts at: 2742 3109 2742 Size: 367 6432 PstI C_TGCA'G Cuts at: 830 2760 2775 3186 4151 830 Size: 1930 15 411 965 3478 Fragments arranged by size: 3478 1930 965 411 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6799 PvuII CAG'CTG Cuts at: 2788 2860 3181 2788 Size: 72 321 6406 Fragments arranged by size: 6406 321 72 SacI G_AGCT'C Cuts at: 2588 2947 3540 2588 Size: 359 593 5847 Fragments arranged by size: 5847 593 359 SalI G'TCGA_C Cuts at: 1936 2825 4141 1936 Size: 889 1316 4594 Fragments arranged by size: 4594 1316 889 SapI GCTCTTCn'nnn_ Cuts at: 2576 2576 Size: 6799 SbfI CC_TGCA'GG Cuts at: 4151 4151 Size: 6799 ScaI AGT'ACT Cuts at: 5047 6759 5047 Size: 1712 5087 SexAI A'CCwGG_T Cuts at: 3915 3915 Size: 6799 SfcI C'TryA_G Cuts at: 826 1045 2756 2771 3182 4147 5282 5963 6154 Size: 219 1711 15 411 965 1135 681 191 Cuts at: 6154 826 Size: 1471 Fragments arranged by size: 1711 1471 1135 965 681 411 219 191 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6799 SmaI CCC'GGG Cuts at: 1872 4126 1872 Size: 2254 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 4016 4903 5804 6066 820 Size: 197 926 876 1197 887 901 262 1553 Fragments arranged by size: 1553 1197 926 901 887 876 262 197 SnaBI TAC'GTA Cuts at: 493 4590 493 Size: 4097 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6799 SphI G_CATG'C Cuts at: 1132 4157 1132 Size: 3025 3774 SspI AAT'ATT Cuts at: 5 52 4723 5 Size: 47 4671 2081 Fragments arranged by size: 4671 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6799 StyI C'CwwG_G Cuts at: 513 1065 2322 2598 4065 4238 513 Size: 552 1257 276 1467 173 3074 Fragments arranged by size: 3074 1467 1257 552 276 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 4826 4843 4996 5181 5626 325 Size: 1255 566 2680 17 153 185 445 1498 Fragments arranged by size: 2680 1498 1255 566 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5045 6757 96 Size: 274 80 33 51 1443 3068 1712 138 Fragments arranged by size: 3068 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6759 6791 6759 Size: 32 6767 Tth111I GACn'n_nGTC Cuts at: 1316 2396 1316 Size: 1080 5719 XbaI T'CTAG_A Cuts at: 4135 4135 Size: 6799 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 3514 2596 Size: 918 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6799 XmaI C'CCGG_G Cuts at: 1870 4124 1870 Size: 2254 4545 XmnI GAAnn'nnTTC Cuts at: 4928 4928 Size: 6799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4607 276 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI