(Linear) (Six-base) MAPSORT of: KIEE6726.seq Check: 2020 from: 1 to: 2094 KIEE6726.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 821 2094 Size: 821 1273 Acc65I G'GTAC_C Cuts at: 0 1166 2094 Size: 1166 928 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 343 526 553 673 875 1342 2094 Size: 212 131 183 27 120 202 467 752 Fragments arranged by size: 752 467 212 202 183 131 120 27 AflII C'TTAA_G Cuts at: 0 814 2094 Size: 814 1280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 803 2094 Size: 803 1291 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1352 1384 2094 Size: 1352 32 710 Fragments arranged by size: 1352 710 32 AlwNI CAG_nnn'CTG Cuts at: 0 783 995 1176 1416 2094 Size: 783 212 181 240 678 Fragments arranged by size: 783 678 240 212 181 ApaI G_GGCC'C Cuts at: 0 741 1108 2094 Size: 741 367 986 Fragments arranged by size: 986 741 367 ApoI r'AATT_y Cuts at: 0 547 1447 2094 Size: 547 900 647 Fragments arranged by size: 900 647 547 AvaI C'yCGr_G Cuts at: 0 842 2094 Size: 842 1252 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 346 379 2094 Size: 346 33 1715 Fragments arranged by size: 1715 346 33 BanI G'GyrC_C Cuts at: 0 1166 2094 Size: 1166 928 BanII G_rGCy'C Cuts at: 0 583 741 942 1088 1108 1535 1838 1999 Size: 583 158 201 146 20 427 303 161 Cuts at: 1999 2094 Size: 95 Fragments arranged by size: 583 427 303 201 161 158 146 95 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 392 1007 1133 1640 2094 Size: 392 615 126 507 454 Fragments arranged by size: 615 507 454 392 126 BbvCI CC'TCA_GC Cuts at: 0 926 2094 Size: 926 1168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 811 845 2094 Size: 811 34 1249 Fragments arranged by size: 1249 811 34 BglII A'GATC_T Cuts at: 0 1328 1820 2094 Size: 1328 492 274 Fragments arranged by size: 1328 492 274 BlpI GC'TnA_GC Cuts at: 0 620 772 932 2094 Size: 620 152 160 1162 Fragments arranged by size: 1162 620 160 152 Bme1580I G_kGCm'C Cuts at: 0 741 1108 2094 Size: 741 367 986 Fragments arranged by size: 986 741 367 BmgBI CAC'GTC Cuts at: 0 165 2094 Size: 165 1929 BmrI ACTGGGnnnn_n' Cuts at: 0 1279 2094 Size: 1279 815 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 597 2094 Size: 597 1497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 567 1117 1249 1897 2094 Size: 567 550 132 648 197 Fragments arranged by size: 648 567 550 197 132 Bpu10I CC'TnA_GC Cuts at: 0 926 1983 2094 Size: 926 1057 111 Fragments arranged by size: 1057 926 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1996 2094 Size: 1996 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 19 2094 Size: 19 2075 BsaWI w'CCGG_w Cuts at: 0 1748 2094 Size: 1748 346 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1825 1855 2094 Size: 1825 30 239 Fragments arranged by size: 1825 239 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 469 772 1023 1480 1852 2094 Size: 469 303 251 457 372 242 Fragments arranged by size: 469 457 372 303 251 242 BseYI C'CCAG_C Cuts at: 0 20 288 335 1089 1096 2094 Size: 20 268 47 754 7 998 Fragments arranged by size: 998 754 268 47 20 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 879 2094 Size: 879 1215 BsiHKAI G_wGCw'C Cuts at: 0 164 583 626 942 1535 2094 Size: 164 419 43 316 593 559 Fragments arranged by size: 593 559 419 316 164 43 BsmI GAATG_Cn' Cuts at: 0 1495 1896 2094 Size: 1495 401 198 Fragments arranged by size: 1495 401 198 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1729 2094 Size: 1729 365 Bsp1286I G_dGCh'C Cuts at: 0 164 583 626 741 942 1088 1108 1535 Size: 164 419 43 115 201 146 20 427 Cuts at: 1535 1838 1999 2094 Size: 303 161 95 Fragments arranged by size: 427 419 303 201 164 161 146 115 95 43 20 BspHI T'CATG_A Cuts at: 0 1898 2094 Size: 1898 196 BsrBI CCG'CTC Cuts at: 0 702 2094 Size: 702 1392 BsrDI GCAATG_nn' Cuts at: 0 1304 1502 2094 Size: 1304 198 592 Fragments arranged by size: 1304 592 198 BstAPI GCAn_nnn'nTGC Cuts at: 0 277 2094 Size: 277 1817 BstEII G'GTnAC_C Cuts at: 0 1654 2094 Size: 1654 440 BstYI r'GATC_y Cuts at: 0 725 1328 1340 1523 1820 2094 Size: 725 603 12 183 297 274 Fragments arranged by size: 725 603 297 274 183 12 Bsu36I CC'TnA_GG Cuts at: 0 439 2094 Size: 439 1655 BtsI GCAGTG_nn' Cuts at: 0 1168 1201 2094 Size: 1168 33 893 Fragments arranged by size: 1168 893 33 DrdI GACnn_nn'nnGTC Cuts at: 0 2053 2094 Size: 2053 41 EaeI y'GGCC_r Cuts at: 0 1753 2094 Size: 1753 341 EarI CTCTTCn'nnn_ Cuts at: 0 128 571 894 1019 2094 Size: 128 443 323 125 1075 Fragments arranged by size: 1075 443 323 128 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 718 2094 Size: 718 1376 EcoICRI GAG'CTC Cuts at: 0 581 940 1533 2094 Size: 581 359 593 561 Fragments arranged by size: 593 581 561 359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 343 526 553 567 673 875 1117 Size: 212 131 183 27 14 106 202 242 Cuts at: 1117 1249 1342 1897 2094 Size: 132 93 555 197 Fragments arranged by size: 555 242 212 202 197 183 132 131 106 93 27 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 814 1267 2094 Size: 814 453 827 Fragments arranged by size: 827 814 453 EcoO109I rG'GnC_Cy Cuts at: 0 36 435 738 957 1104 1215 1551 2094 Size: 36 399 303 219 147 111 336 543 Fragments arranged by size: 543 399 336 303 219 147 111 36 EcoRI G'AATT_C Cuts at: 0 547 2094 Size: 547 1547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 79 829 1347 2037 2094 Size: 79 750 518 690 57 Fragments arranged by size: 750 690 518 79 57 HaeII r_GCGC'y Cuts at: 0 1386 1809 2091 2094 Size: 1386 423 282 3 Fragments arranged by size: 1386 423 282 3 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 259 261 291 478 510 888 920 Size: 229 30 2 30 187 32 378 32 Cuts at: 920 1707 1739 1767 1799 1825 1857 2094 Size: 787 32 28 32 26 32 237 Fragments arranged by size: 787 378 237 229 187 32 32 32 32 32 30 30 28 26 2 HincII GTy'rAC Cuts at: 0 822 1205 2094 Size: 822 383 889 Fragments arranged by size: 889 822 383 KpnI G_GTAC'C Cuts at: 0 1170 2094 Size: 1170 924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 74 2094 Size: 74 2020 MscI TGG'CCA Cuts at: 0 1755 2094 Size: 1755 339 MslI CAynn'nnrTG Cuts at: 0 1156 1285 1784 2094 Size: 1156 129 499 310 Fragments arranged by size: 1156 499 310 129 MspA1I CmG'CkG Cuts at: 0 783 855 1176 2094 Size: 783 72 321 918 Fragments arranged by size: 918 783 321 72 NdeI CA'TA_TG Cuts at: 0 176 1758 2094 Size: 176 1582 336 Fragments arranged by size: 1582 336 176 NspI r_CATG'y Cuts at: 0 1710 2094 Size: 1710 384 PflMI CCAn_nnn'nTGG Cuts at: 0 783 2094 Size: 783 1311 PfoI T'CCnGG_A Cuts at: 0 551 1519 1874 2094 Size: 551 968 355 220 Fragments arranged by size: 968 551 355 220 PpuMI rG'GwC_Cy Cuts at: 0 957 1551 2094 Size: 957 594 543 Fragments arranged by size: 957 594 543 PshAI GACnn'nnGTC Cuts at: 0 56 2094 Size: 56 2038 PspOMI G'GGCC_C Cuts at: 0 737 1104 2094 Size: 737 367 990 Fragments arranged by size: 990 737 367 PstI C_TGCA'G Cuts at: 0 755 770 1181 2094 Size: 755 15 411 913 Fragments arranged by size: 913 755 411 15 PvuII CAG'CTG Cuts at: 0 783 855 1176 2094 Size: 783 72 321 918 Fragments arranged by size: 918 783 321 72 SacI G_AGCT'C Cuts at: 0 583 942 1535 2094 Size: 583 359 593 559 Fragments arranged by size: 593 583 559 359 SalI G'TCGA_C Cuts at: 0 820 2094 Size: 820 1274 SapI GCTCTTCn'nnn_ Cuts at: 0 571 2094 Size: 571 1523 SexAI A'CCwGG_T Cuts at: 0 1910 2094 Size: 1910 184 SfcI C'TryA_G Cuts at: 0 751 766 1177 2094 Size: 751 15 411 917 Fragments arranged by size: 917 751 411 15 SmlI C'TyrA_G Cuts at: 0 814 2011 2094 Size: 814 1197 83 Fragments arranged by size: 1197 814 83 StyI C'CwwG_G Cuts at: 0 317 593 2060 2094 Size: 317 276 1467 34 Fragments arranged by size: 1467 317 276 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 141 2094 Size: 141 1953 Tth111I GACn'n_nGTC Cuts at: 0 391 2094 Size: 391 1703 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 591 1509 2094 Size: 591 918 585 Fragments arranged by size: 918 591 585 Enzymes that do cut: AccI Acc65I AcuI AflII AlfI AloI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BstAPI BstEII BstYI Bsu36I BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NdeI NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SalI SapI SexAI SfcI SmlI StyI TaqII Tth111I XcmI Enzymes that do not cut: AarI AatII AclI AfeI AflIII AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BciVI BclI BfrBI BglI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI BspMI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EagI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI