(Circular) (Six-base) MAPSORT of: pFN21AE1394.seq Check: 2938 from: 1 to: 6271 pFN21AE1394 6271 bp With 182 enzymes: SgfI * January 28, 2010 16:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 3157 2677 Size: 480 5791 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3069 3076 3261 3476 4334 5718 5986 1632 Size: 1437 7 185 215 858 1384 268 1917 Fragments arranged by size: 1917 1437 1384 858 268 215 185 7 AflII C'TTAA_G Cuts at: 820 1017 2521 820 Size: 197 1504 4570 Fragments arranged by size: 4570 1504 197 AflIII A'CryG_T Cuts at: 1203 5170 6147 1203 Size: 3967 977 1327 Fragments arranged by size: 3967 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 2712 1166 Size: 1546 4725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2678 4014 5586 2678 Size: 1336 1572 3363 Fragments arranged by size: 3363 1572 1336 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2051 2157 3307 3580 3806 3958 6067 1493 Size: 558 106 1150 273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1150 558 273 226 152 106 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3596 1870 Size: 73 1653 4545 Fragments arranged by size: 4545 1653 73 BamHI G'GATC_C Cuts at: 2202 2722 3601 3968 2202 Size: 520 879 367 4505 Fragments arranged by size: 4505 879 520 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3592 5047 618 Size: 325 217 627 1805 1455 1842 Fragments arranged by size: 1842 1805 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3590 1391 Size: 2199 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2604 3015 928 Size: 971 705 411 4184 Fragments arranged by size: 4184 971 705 411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BfrBI ATG'CAT Cuts at: 2104 2104 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 4882 136 Size: 107 122 71 631 605 580 2630 1525 Fragments arranged by size: 2630 1525 631 605 580 122 107 71 BglII A'GATC_T Cuts at: 2489 6266 2489 Size: 3777 2494 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 4332 5488 1790 Size: 2542 1156 2573 Fragments arranged by size: 2573 2542 1156 BmgBI CAC'GTC Cuts at: 1318 2926 1318 Size: 1608 4663 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2759 4002 4960 Size: 416 53 83 399 24 1342 1243 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1753 1342 1243 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 3260 4931 1239 Size: 933 1088 1671 2579 Fragments arranged by size: 2579 1671 1088 933 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4396 5261 5559 4396 Size: 865 298 5108 Fragments arranged by size: 5108 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4934 882 Size: 617 3435 2219 Fragments arranged by size: 3435 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2689 4062 493 Size: 711 1485 1373 2702 Fragments arranged by size: 2702 1485 1373 711 BsaBI GATnn'nnATC Cuts at: 2201 6265 2201 Size: 4064 2207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2170 2200 2292 2322 1178 Size: 30 165 30 767 30 92 30 5127 Fragments arranged by size: 5127 767 165 92 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 2903 3257 1186 Size: 989 728 354 4200 Fragments arranged by size: 4200 989 728 354 BseYI C'CCAG_C Cuts at: 5474 5970 5474 Size: 496 5775 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3148 4332 4417 5488 3148 Size: 1184 85 1071 3931 Fragments arranged by size: 3931 1184 1071 85 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3148 3590 4332 4417 5488 1391 Size: 399 1358 442 742 85 1071 2174 Fragments arranged by size: 2174 1358 1071 742 442 399 85 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 2757 4160 5890 2757 Size: 1403 1730 3138 Fragments arranged by size: 3138 1730 1403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2677 3157 3626 844 Size: 1833 480 469 3489 Fragments arranged by size: 3489 1833 480 469 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2086 2232 2451 2736 3499 4766 4940 Size: 1183 838 146 219 285 763 1267 174 Cuts at: 4940 65 Size: 1396 Fragments arranged by size: 1396 1267 1183 838 763 285 219 174 146 BsrFI r'CCGG_y Cuts at: 1700 1810 3040 4915 5102 1700 Size: 110 1230 1875 187 2869 Fragments arranged by size: 2869 1875 1230 187 110 BsrGI T'GTAC_A Cuts at: 96 3297 96 Size: 3201 3070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6271 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2202 2489 2618 2722 3470 3601 3968 4353 Size: 230 287 129 104 748 131 367 385 Cuts at: 4353 4370 5822 5908 5920 6266 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 748 385 367 346 287 230 131 129 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2433 2970 513 Size: 552 1368 537 3814 Fragments arranged by size: 3814 1368 552 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3787 4581 4601 3787 Size: 794 20 5457 Fragments arranged by size: 5457 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 3718 4607 Size: 54 1425 50 275 18 600 1288 889 Cuts at: 4607 6236 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1288 889 600 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2986 3154 3414 4201 1353 Size: 1633 168 260 787 3423 Fragments arranged by size: 3423 1633 787 260 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 3069 3076 3260 3261 3476 4334 Size: 393 540 897 7 184 1 215 858 Cuts at: 4334 4931 5718 5986 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 897 858 787 597 540 393 268 215 184 7 1 EcoNI CCTnn'n_nnAGG Cuts at: 2322 2322 Size: 6271 EcoRI G'AATT_C Cuts at: 2051 3580 3958 2051 Size: 1529 378 4364 Fragments arranged by size: 4364 1529 378 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 5418 1678 Size: 3740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 2170 2202 4912 Size: 32 135 32 531 32 35 32 2710 Cuts at: 4912 4944 4986 5018 1373 Size: 32 42 32 2626 Fragments arranged by size: 2710 2626 531 135 42 35 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3615 3861 669 Size: 988 281 1024 653 246 3079 Fragments arranged by size: 3079 1024 988 653 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 2574 3870 2574 Size: 1296 4975 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3526 5385 5569 1305 Size: 2221 1859 184 2007 Fragments arranged by size: 2221 2007 1859 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2432 10 Size: 54 1425 343 600 3849 Fragments arranged by size: 3849 1425 600 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2558 2712 2862 4229 4588 4747 Size: 648 1056 336 154 150 1367 359 159 Cuts at: 4747 518 Size: 2042 Fragments arranged by size: 2042 1367 1056 648 359 336 159 154 150 MspA1I CmG'CkG Cuts at: 1606 3114 3687 4364 5512 5757 1606 Size: 1508 573 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 573 245 NcoI C'CATG_G Cuts at: 513 1065 2433 2970 513 Size: 552 1368 537 3814 Fragments arranged by size: 3814 1368 552 537 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NsiI A_TGCA'T Cuts at: 2106 2106 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2108 3629 5174 1132 Size: 97 879 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 879 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6271 PfoI T'CCnGG_A Cuts at: 2188 2188 Size: 6271 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 2486 3841 2486 Size: 1355 4916 PstI C_TGCA'G Cuts at: 830 2674 2914 3478 3623 830 Size: 1844 240 564 145 3478 Fragments arranged by size: 3478 1844 564 240 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SapI GCTCTTCn'nnn_ Cuts at: 3154 3154 Size: 6271 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2670 2910 3323 3403 3474 3619 4754 Size: 219 1625 240 413 80 71 145 1135 Cuts at: 4754 5435 5626 826 Size: 681 191 1471 Fragments arranged by size: 1625 1471 1135 681 413 240 219 191 145 80 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2521 4375 5276 5538 820 Size: 197 926 578 1854 901 262 1553 Fragments arranged by size: 1854 1553 926 901 578 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 2839 4195 5 Size: 47 2787 1356 2081 Fragments arranged by size: 2787 2081 1356 47 StuI AGG'CCT Cuts at: 1517 2017 2044 1517 Size: 500 27 5744 Fragments arranged by size: 5744 500 27 StyI C'CwwG_G Cuts at: 513 1065 2433 2970 3710 513 Size: 552 1368 537 740 3074 Fragments arranged by size: 3074 1368 740 552 537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4298 4315 4468 4653 5098 325 Size: 1255 2718 17 153 185 445 1498 Fragments arranged by size: 2718 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2746 3297 3423 Size: 274 80 33 51 1443 769 551 126 Cuts at: 3423 4517 6229 96 Size: 1094 1712 138 Fragments arranged by size: 1712 1443 1094 769 551 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2673 2705 3028 3060 6231 6263 2673 Size: 32 323 32 3171 32 2681 Fragments arranged by size: 3171 2681 323 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 2160 3054 4400 2160 Size: 894 1346 4031 Fragments arranged by size: 4031 1346 894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI