################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 505 CGCG CG^CG Alw44I 1033 GTGCAC G^TGCAC AlwNI 372 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1033 GTGCAC G^TGCAC AspHI 1033 GTGCAC G[AT]GC[AT]^C BalI 665 TGGCCA TGG^CCA BanII 548 GAGCCC G[GA]GC[CT]^C BbvCI 344 CCTCAGC CCTCAGC(-5/-2) BglI 284 GCCTTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiHKAI 1033 GTGCAC G[AT]GC[AT]^C BsmFI 839 GGGAC GGGAC(10/14) BsmI 223 GAATGC GAATGC(1/-1) BspHI 734 TCATGA T^CATGA BsrBI 633 GAGCGG CCGCTC(-3/-3) BssSI 802 CTCGTG CACGAG(-5/-1) BstUI 505 CGCG CG^CG BstXI 722 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 625 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 72 CTCTTC CTCTTC(1/4) EcoRI 816 GAATTC G^AATTC FauI 503 CCCGC CCCGC(4/6) HaeII 430 AGCGCC [GA]GCGC^[CT] HincII 67 GTCAAC GT[CT]^[GA]AC HindII 67 GTCAAC GT[CT]^[GA]AC Ksp632I 72 CTCTTC CTCTTC(1/4) MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA MseI 779 TTAA T^TAA MvnI 505 CGCG CG^CG NcoI 668 CCATGG C^CATGG PflMI 198 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 296 GGGTCCT [GA]G^G[AT]CC[CT] PstI 468 CTGCAG CTGCA^G RcaI 734 TCATGA T^CATGA SmaI 893 CCCGGG CCC^GGG StuI 135 AGGCCT AGG^CCT Tru9I 779 TTAA T^TAA Van91I 198 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 893 CCCGGG C^CCGGG XmaI 893 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 496 AAATTT [GA]^AATT[CT] 816 GAATTC ApoI 496 AAATTT [GA]^AATT[CT] 816 GAATTC AvaI 61 CCCGAG C^[CT]CG[GA]G 893 CCCGGG AvaII 297 GGTCC G^G[AT]CC 840 GGACC BmyI 548 GAGCCC G[AGT]GC[ACT]^C 1033 GTGCAC BseRI 105 CTCCTC GAGGAG(10/8) 342 CTCCTC BsoBI 61 CCCGAG C^[CT]CG[GA]G 893 CCCGGG Bsp1286I 548 GAGCCC G[AGT]GC[ACT]^C 1033 GTGCAC DdeI 345 CTCAG C^T[ACGT]AG 649 CTCAG DsaI 624 CCACGG C^C[GA][CT]GG 668 CCATGG HapII 894 CCGG C^CGG 957 CCGG HpaII 894 CCGG C^CGG 957 CCGG MaeIII 164 GTCAC ^GT[ACGT]AC 952 GTCAC MspI 894 CCGG C^CGG 957 CCGG NspI 655 ACATGC [GA]CATG^[CT] 1039 ACATGC Tsp45I 164 GTCAC ^GT[GC]AC 952 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MnlI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp509I TspRI