(Circular) (Six-base) MAPSORT of: pFN21AE1395.seq Check: 9183 from: 1 to: 5785 pFN21AE1395 5785 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2668 3848 5232 5500 1632 Size: 1036 1180 1384 268 1917 Fragments arranged by size: 1917 1384 1180 1036 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2382 3528 5100 2382 Size: 1146 1572 3067 Fragments arranged by size: 3067 1572 1146 ApaLI G'TGCA_C Cuts at: 3038 3842 4998 3038 Size: 804 1156 3825 Fragments arranged by size: 3825 1156 804 ApoI r'AATT_y Cuts at: 1493 2501 2821 3094 3320 3472 5581 1493 Size: 1008 320 273 226 152 2109 1697 Fragments arranged by size: 2109 1697 1008 320 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2898 3110 1870 Size: 73 123 832 212 4545 Fragments arranged by size: 4545 832 212 123 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2821 2854 2821 Size: 33 5752 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3106 4561 618 Size: 325 217 627 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 627 325 217 BanII G_rGCy'C Cuts at: 1391 2557 3104 1391 Size: 1166 547 4072 Fragments arranged by size: 4072 1166 547 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4814 BbvCI CC'TCA_GC Cuts at: 2350 2350 Size: 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 4396 136 Size: 107 122 71 631 605 623 2101 1525 Fragments arranged by size: 2101 1525 631 623 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 3042 3846 5002 1790 Size: 1252 804 1156 2573 Fragments arranged by size: 2573 1252 1156 804 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2875 2092 Size: 783 5002 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2376 2728 2811 4445 1239 Size: 859 278 352 83 1634 2579 Fragments arranged by size: 2579 1634 859 352 278 83 Bpu10I CC'TnA_GC Cuts at: 2350 2350 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2933 2963 1178 Size: 30 165 30 1530 30 4000 Fragments arranged by size: 4000 1530 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2338 1186 Size: 915 237 4633 Fragments arranged by size: 4633 915 237 BseYI C'CCAG_C Cuts at: 4988 5484 4988 Size: 496 5289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3042 3846 3931 5002 3042 Size: 804 85 1071 3825 Fragments arranged by size: 3825 1071 804 85 BsmI GAATG_Cn' Cuts at: 2234 3296 3389 2234 Size: 1062 93 4630 Fragments arranged by size: 4630 1062 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2557 3042 3104 3846 3931 5002 1391 Size: 399 767 485 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 485 399 85 62 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 2739 3674 5404 2739 Size: 935 1730 3120 Fragments arranged by size: 3120 1730 935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3140 844 Size: 2296 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2640 3672 1394 Size: 429 817 1032 3507 Fragments arranged by size: 3507 1032 817 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 2807 3845 4857 2807 Size: 1038 1012 3735 Fragments arranged by size: 3735 1038 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2734 Size: 5785 BstYI r'GATC_y Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780 Size: 1143 367 385 17 1452 86 12 346 Cuts at: 5780 1972 Size: 1977 Fragments arranged by size: 1977 1452 1143 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2629 2673 513 Size: 552 1564 44 3625 Fragments arranged by size: 3625 1564 552 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2482 3301 4095 4115 2482 Size: 819 794 20 4152 Fragments arranged by size: 4152 819 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DraIII CAC_nnn'GTG Cuts at: 2635 2635 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2670 2676 Size: 54 1425 50 275 18 381 459 6 Cuts at: 2676 3232 4121 5750 8 Size: 556 889 1629 43 Fragments arranged by size: 1629 1425 889 556 459 381 275 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2083 3715 1353 Size: 730 1632 3423 Fragments arranged by size: 3423 1632 730 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 4372 4746 4892 387 Size: 1064 138 645 2138 374 146 1280 Fragments arranged by size: 2138 1280 1064 645 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2376 2668 2728 2811 3848 4445 Size: 393 466 278 292 60 83 1037 597 Cuts at: 4445 5232 5500 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1037 787 597 466 393 292 278 268 83 60 EcoO109I rG'GnC_Cy Cuts at: 2302 2379 3052 2302 Size: 77 673 5035 Fragments arranged by size: 5035 673 77 EcoRI G'AATT_C Cuts at: 2821 3094 3472 2821 Size: 273 378 5134 Fragments arranged by size: 5134 378 273 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2439 4932 1678 Size: 761 2493 2531 Fragments arranged by size: 2531 2493 761 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373 Size: 32 135 32 2854 32 42 32 2626 Fragments arranged by size: 2854 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2074 3129 3375 669 Size: 988 281 136 1055 246 3079 Fragments arranged by size: 3079 1055 988 281 246 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 10 Size: 54 1425 343 840 3123 Fragments arranged by size: 3123 1425 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2732 2963 3743 4102 4261 518 Size: 648 1015 551 231 780 359 159 2042 Fragments arranged by size: 2042 1015 780 648 551 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2158 2164 2256 2354 2913 3201 3878 5026 Size: 552 6 92 98 559 288 677 1148 Cuts at: 5026 5271 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 559 552 288 245 98 92 6 NcoI C'CATG_G Cuts at: 513 1065 2673 513 Size: 552 1608 3625 Fragments arranged by size: 3625 1608 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 2664 3048 3143 4688 1132 Size: 97 1435 384 95 1545 2229 Fragments arranged by size: 2229 1545 1435 384 97 95 PasI CC'CwG_GG Cuts at: 1348 2863 1348 Size: 1515 4270 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 1108 Size: 1101 4684 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 2302 Size: 5785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 2477 3137 830 Size: 1647 660 3478 Fragments arranged by size: 3478 1647 660 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2164 2354 2913 2164 Size: 190 559 5036 Fragments arranged by size: 5036 559 190 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2100 2383 2473 3081 3133 4268 4949 Size: 219 1055 283 90 608 52 1135 681 Cuts at: 4949 5140 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1055 681 608 283 219 191 90 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 2900 3112 1872 Size: 1028 212 4545 Fragments arranged by size: 4545 1028 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 2142 1517 Size: 625 5160 StyI C'CwwG_G Cuts at: 513 1065 2389 2439 2673 3224 513 Size: 552 1324 50 234 551 3074 Fragments arranged by size: 3074 1324 552 551 234 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2862 2967 3812 3829 3982 4167 4612 Size: 1255 1282 105 845 17 153 185 445 Cuts at: 4612 325 Size: 1498 Fragments arranged by size: 1498 1282 1255 845 445 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2648 2715 4031 Size: 274 80 33 51 1443 671 67 1316 Cuts at: 4031 5743 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1316 671 274 138 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 2898 3110 1870 Size: 1028 212 4545 Fragments arranged by size: 4545 1028 212 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI