Restriction map for
pFN21AE1355
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII EaeI EagI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspI StyI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 514 GTCTAC GT^[AC][GT]AC
AfaI 315 GTAC GT^AC
AhdI 325 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 325 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 474 CCCGGG C^[CT]CG[GA]G
AvaII 174 GGTCC G^G[AT]CC
BanI 733 GGCGCC G^G[CT][GA]CC
BanII 370 GAGCCC G[GA]GC[CT]^C
BbeI 733 GGCGCC GGCGC^C
BcgI 567 CGACTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 370 GAGCCC G[AGT]GC[ACT]^C
BsaAI 259 TACGTG [CT]AC^GT[GA]
BsgI 142 GTGCAG GTGCAG(16/14)
BsoBI 474 CCCGGG C^[CT]CG[GA]G
Bsp1286I 370 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 314 TGTACA T^GTACA
BsrGI 314 TGTACA T^GTACA
BstXI 90 CCAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 146 AGATCC [GA]^GATC[CT]
DraIII 256 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 105 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 206 CCGTGG C^C[GA][CT]GG
Eam1105I 325 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 755 CTCTTC CTCTTC(1/4)
HinfI 748 GACTC G^A[ACGT]TC
HphI 834 GGTGA GGTGA(8/7)
KasI 733 GGCGCC G^GCGCC
Ksp632I 755 CTCTTC CTCTTC(1/4)
MflI 146 AGATCC [GA]^GATC[CT]
MseI 764 TTAA T^TAA
MslI 555 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 733 GGCGCC GG^CGCC
NspI 465 GCATGC [GA]CATG^[CT]
PleI 748 GACTC GAGTC(4/5)
RsaI 315 GTAC GT^AC
SapI 754 GCTCTTC GCTCTTC(1/4)
SexAI 326 ACCAGGT A^CC[AT]GGT
SfoI 733 GGCGCC GGC^GCC
SmaI 474 CCCGGG CCC^GGG
SphI 465 GCATGC GCATG^C
SspBI 314 TGTACA T^GTACA
StuI 319 AGGCCT AGG^CCT
TaqI 497 TCGA T^CGA
Tru9I 764 TTAA T^TAA
TthHB8I 497 TCGA T^CGA
XcmI 771 CCAGGCTGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 146 AGATCC [GA]^GATC[CT]
XmaCI 474 CCCGGG C^CCGGG
XmaI 474 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AatII 511 GACGTC GACGT^C
550 GACGTC
AlwNI 145 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
643 CAGTGGCTG
Aor51HI 299 AGCGCT AGC^GCT
661 AGCGCT
BsaI 103 GAGACC GGTCTC(1/5)
594 GAGACC
DdeI 151 CTGAG C^T[ACGT]AG
787 CTGAG
Eco47III 299 AGCGCT AGC^GCT
661 AGCGCT
FauI 377 GCGGG CCCGC(4/6)
577 GCGGG
HapII 426 CCGG C^CGG
475 CCGG
HgaI 481 GACGC GACGC(5/10)
499 GACGC
HpaII 426 CCGG C^CGG
475 CCGG
MaeIII 791 GTGAC ^GT[ACGT]AC
815 GTGAC
MspI 426 CCGG C^CGG
475 CCGG
PstI 49 CTGCAG CTGCA^G
582 CTGCAG
SfcI 49 CTGCAG C^T[GA][CT]AG
582 CTGCAG
Tsp45I 791 GTGAC ^GT[GC]AC
815 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcyI AluI AlwI BbvI
BcnI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoNI EcoRII Fnu4HI FokI HaeII
HaeIII HhaI Hin1I HinP1I ItaI
MaeII MboI MboII MnlI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI