################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MflI MluI MluNI MroI MscI MslI MspA1I MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AciI 19 GCGG CCGC(-3/-1) AfaI 306 GTAC GT^AC AvaII 125 GGACC G^G[AT]CC BbsI 4 GAAGAC GAAGAC(2/6) BpuAI 4 GAAGAC GAAGAC(2/6) BseRI 22 GAGGAG GAGGAG(10/8) BsgI 276 CTGCAC GTGCAG(16/14) BsmAI 25 GAGAC GTCTC(1/5) BsmFI 254 GGGAC GGGAC(10/14) BsrI 120 CCAGT ACTGG(1/-1) BssKI 12 CCAGG ^CC[ACGT]GG BstNI 12 CCAGG CC^[AT]GG CfoI 80 GCGC GCG^C Cfr13I 125 GGACC G^G[ACGT]CC DraI 181 TTTAAA TTT^AAA EcoRII 12 CCAGG ^CC[AT]GG HhaI 80 GCGC GCG^C HinP1I 80 GCGC G^CGC HphI 161 TCACC GGTGA(8/7) MfeI 83 CAATTG C^AATTG MunI 83 CAATTG C^AATTG MvaI 12 CCAGG CC^[AT]GG NlaIII 303 CATG CATG^ RsaI 306 GTAC GT^AC Sau96I 125 GGACC G^G[ACGT]CC ScrFI 12 CCAGG CC^[ACGT]GG SfaNI 311 GCATC GCATC(5/9) SfcI 71 CTATAG C^T[GA][CT]AG SwaI 180 ATTTAAAT ATTT^AAAT Tsp45I 193 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 16 GCTGC GCAGC(8/12) 275 GCTGC BsiYI 12 CCAGGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 245 CCTGCTTCTGG BslI 12 CCAGGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 245 CCTGCTTCTGG Fnu4HI 16 GCTGC GC^[ACGT]GC 275 GCTGC ItaI 16 GCTGC GC^[ACGT]GC 275 GCTGC MaeIII 92 GTAAC ^GT[ACGT]AC 193 GTGAC MboII 4 GAAGA GAAGA(8/7) 213 GAAGA MnlI 22 GAGG CCTC(7/6) 67 GAGG MseI 151 TTAA T^TAA 182 TTAA Tru9I 151 TTAA T^TAA 182 TTAA TseI 16 GCTGC G^C[AT]GC 275 GCTGC Tsp509I 84 AATT ^AATT 204 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### AluI TspRI