################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstF5I BstZ17I CelII ClaI CpoI DraI DrdI DsaI Eam1105I EarI EcoNI EcoRV EcoT22I FbaI FokI FseI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MboII MluI MroI MslI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 231 GAATTC [GA]^AATT[CT] AcyI 74 GACGCC G[GA]^CG[CT]C AfaI 777 GTAC GT^AC AocI 501 CCTGAGG CC^T[ACGT]AGG Aor51HI 542 AGCGCT AGC^GCT ApoI 231 GAATTC [GA]^AATT[CT] AspHI 321 GAGCTC G[AT]GC[AT]^C AvaI 51 CCCGGG C^[CT]CG[GA]G BalI 563 TGGCCA TGG^CCA BamHI 752 GGATCC G^GATCC BanI 675 GGCACC G^G[CT][GA]CC BbrPI 460 CACGTG CAC^GTG BcgI 585 CGAGTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 793 CTAG C^TAG BsaAI 460 CACGTG [CT]AC^GT[GA] BsaHI 74 GACGCC G[GA]^CG[CT]C BsaI 5 GGTCTC GGTCTC(1/5) BseRI 757 CTCCTC GAGGAG(10/8) BsiEI 41 CGGCCG CG[GA][CT]^CG BsiHKAI 321 GAGCTC G[AT]GC[AT]^C BsmAI 6 GTCTC GTCTC(1/5) BsoBI 51 CCCGGG C^[CT]CG[GA]G BsrFI 550 ACCGGC [GA]^CCGG[CT] BssSI 340 CTCGTG CACGAG(-5/-1) BstEII 495 GGTGACC G^GT[ACGT]ACC BstPI 495 GGTGACC G^GT[ACGT]ACC BstXI 713 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 501 CCTGAGG CC^T[ACGT]AGG Cfr10I 550 ACCGGC [GA]^CCGG[CT] DraIII 403 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 41 CGGCCG C^GGCCG EclXI 41 CGGCCG C^GGCCG Eco47III 542 AGCGCT AGC^GCT Eco52I 41 CGGCCG C^GGCCG Eco81I 501 CCTGAGG CC^T[ACGT]AGG EcoO65I 495 GGTGACC G^GT[ACGT]ACC EcoRI 231 GAATTC G^AATTC EcoT14I 101 CCAAGG C^C[AT][AT]GG Hin1I 74 GACGCC G[GA]^CG[CT]C MaeI 793 CTAG C^TAG MfeI 201 CAATTG C^AATTG MluNI 563 TGGCCA TGG^CCA MscI 563 TGGCCA TGG^CCA MseI 430 TTAA T^TAA MunI 201 CAATTG C^AATTG NotI 40 GCGGCCGC GC^GGCCGC PmaCI 460 CACGTG CAC^GTG PmlI 460 CACGTG CAC^GTG PpuMI 127 GGGACCT [GA]G^G[AT]CC[CT] RsaI 777 GTAC GT^AC SacI 321 GAGCTC GAGCT^C SfaNI 690 GATGC GCATC(5/9) SmaI 51 CCCGGG CCC^GGG StyI 101 CCAAGG C^C[AT][AT]GG Tru9I 430 TTAA T^TAA XmaCI 51 CCCGGG C^CCGGG XmaI 51 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AviII 149 TGCGCA TGC^GCA 349 TGCGCA BsaWI 423 TCCGGT [AT]^CCGG[AT] 727 TCCGGT BsgI 175 GTGCAG GTGCAG(16/14) 193 GTGCAG BspMI 346 ACCTGC ACCTGC(4/8) 468 GCAGGT BssHII 65 GCGCGC G^CGCGC 634 GCGCGC BstYI 558 GGATCT [GA]^GATC[CT] 752 GGATCC DraII 127 GGGACCT [GA]G^G[ACGT]CC[CT] 313 AGGGCCT EaeI 41 CGGCCG [CT]^GGCC[GA] 563 TGGCCA EcoO109I 127 GGGACCT [GA]G^G[ACGT]CC[CT] 313 AGGGCCT FspI 149 TGCGCA TGC^GCA 349 TGCGCA HaeII 542 AGCGCT [GA]GCGC^[CT] 769 GGCGCT MflI 558 GGATCT [GA]^GATC[CT] 752 GGATCC NlaIII 153 CATG CATG^ 508 CATG PvuII 213 CAGCTG CAG^CTG 298 CAGCTG TaqI 305 TCGA T^CGA 647 TCGA Tsp509I 202 AATT ^AATT 232 AATT TthHB8I 305 TCGA T^CGA 647 TCGA XhoII 558 GGATCT [GA]^GATC[CT] 752 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I TspRI