(Circular) (Six-base) MAPSORT of: pFN21AE6214.seq Check: 2994 from: 1 to: 5518 pFN21AE6214 5518 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2464 2464 Size: 5518 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3581 4965 5233 1632 Size: 1949 1384 268 1917 Fragments arranged by size: 1949 1917 1384 268 AfeI AGC'GCT Cuts at: 2549 2549 Size: 5518 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 1184 Size: 1089 4429 AlwNI CAG_nnn'CTG Cuts at: 3261 4833 3261 Size: 1572 3946 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2236 2827 3053 3205 5314 1493 Size: 743 591 226 152 2109 1697 Fragments arranged by size: 2109 1697 743 591 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2843 1870 Size: 73 113 787 4545 Fragments arranged by size: 4545 787 113 73 BamHI G'GATC_C Cuts at: 2757 2848 3215 2757 Size: 91 367 5060 Fragments arranged by size: 5060 367 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 2839 4294 618 Size: 325 217 627 893 159 1455 1842 Fragments arranged by size: 1842 1455 893 627 325 217 159 BanII G_rGCy'C Cuts at: 1391 2165 2330 2711 2837 1391 Size: 774 165 381 126 4072 Fragments arranged by size: 4072 774 381 165 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2579 2613 3709 3743 1903 Size: 34 642 34 1096 34 3678 Fragments arranged by size: 3678 1096 642 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 2145 3579 4735 1790 Size: 355 1434 1156 2573 Fragments arranged by size: 2573 1434 1156 355 BmgBI CAC'GTC Cuts at: 1318 2314 1318 Size: 996 4522 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2344 2365 2564 4178 1239 Size: 1105 21 199 1614 2579 Fragments arranged by size: 2579 1614 1105 199 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2016 4181 882 Size: 617 517 2165 2219 Fragments arranged by size: 2219 2165 617 517 BsaAI yAC'GTr Cuts at: 493 1204 2467 3309 493 Size: 711 1263 842 2702 Fragments arranged by size: 2702 1263 842 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 3325 3707 4342 Size: 53 83 186 936 547 1245 382 635 Cuts at: 4342 275 Size: 1451 Fragments arranged by size: 1451 1245 936 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2428 2732 3951 4349 4623 4770 5181 2428 Size: 304 1219 398 274 147 411 2765 Fragments arranged by size: 2765 1219 411 398 304 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5293 Fragments arranged by size: 5293 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2753 1186 Size: 1567 3951 BseYI C'CCAG_C Cuts at: 2074 2226 2370 4721 5217 2074 Size: 152 144 2351 496 2375 Fragments arranged by size: 2375 2351 496 152 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 2219 1163 Size: 573 465 18 4462 Fragments arranged by size: 4462 573 465 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2968 3729 4757 1540 Size: 275 186 48 919 761 1028 2301 Fragments arranged by size: 2301 1028 919 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2330 3579 3664 4735 2330 Size: 1249 85 1071 3113 Fragments arranged by size: 3113 1249 1071 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2165 2330 2711 2837 3579 3664 Size: 399 355 20 165 381 126 742 85 Cuts at: 3664 4735 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 381 355 165 126 85 20 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 2464 2873 844 Size: 1516 104 409 3489 Fragments arranged by size: 3489 1516 409 104 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2555 4162 4349 1700 Size: 110 745 1607 187 2869 Fragments arranged by size: 2869 1607 745 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 2070 2639 1925 Size: 145 569 4804 Fragments arranged by size: 4804 569 145 BssSI C'ACGA_G Cuts at: 2345 3578 4590 2345 Size: 1233 1012 3273 Fragments arranged by size: 3273 1233 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 2500 1184 Size: 1316 4202 BstXI CCAn_nnnn'nTGG Cuts at: 2725 2725 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2563 2757 2848 3215 3600 3617 5069 5155 Size: 591 194 91 367 385 17 1452 86 Cuts at: 5155 5167 5513 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 591 385 367 346 194 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2507 2507 Size: 5518 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2725 487 Size: 683 56 1499 3280 Fragments arranged by size: 3280 1499 683 56 BtsI GCAGTG_nn' Cuts at: 2338 3034 3828 3848 2338 Size: 696 794 20 4008 Fragments arranged by size: 4008 794 696 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DraIII CAC_nnn'GTG Cuts at: 2413 2413 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2568 2965 Size: 54 1425 50 275 18 216 522 397 Cuts at: 2965 3854 5483 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 522 397 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2965 1537 Size: 275 234 919 4090 Fragments arranged by size: 4090 919 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 3448 1353 Size: 2095 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2328 2328 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2344 2365 2564 3581 4178 4965 5233 Size: 393 712 21 199 1017 597 787 268 Cuts at: 5233 1239 Size: 1524 Fragments arranged by size: 1524 1017 787 712 597 393 268 199 21 EcoO109I rG'GnC_Cy Cuts at: 2133 2319 2133 Size: 186 5332 EcoRI G'AATT_C Cuts at: 2236 2827 3205 2236 Size: 591 378 4549 Fragments arranged by size: 4549 591 378 FspI TGC'GCA Cuts at: 2156 2356 4024 2156 Size: 200 1668 3650 Fragments arranged by size: 3650 1668 200 FspAI rTGC'GCAy Cuts at: 2156 2156 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 2551 2778 4665 1678 Size: 873 227 1887 2531 Fragments arranged by size: 2531 1887 873 227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373 Size: 32 135 32 2587 32 42 32 2626 Fragments arranged by size: 2626 2587 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 2206 3117 2206 Size: 911 4607 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 10 Size: 54 1425 343 738 2958 Fragments arranged by size: 2958 1425 738 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 2257 2305 2934 3611 4759 5004 1606 Size: 614 37 48 629 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 629 614 245 48 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2046 2965 2046 Size: 919 4599 NspI r_CATG'y Cuts at: 1132 1229 2876 4421 1132 Size: 97 1647 1545 2229 Fragments arranged by size: 2229 1647 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PfoI T'CCnGG_A Cuts at: 2129 2381 2129 Size: 252 5266 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PmlI CAC'GTG Cuts at: 2467 2467 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2133 2133 Size: 5518 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PstI C_TGCA'G Cuts at: 830 2870 830 Size: 2040 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2220 2305 2220 Size: 85 5433 SacI G_AGCT'C Cuts at: 2330 2330 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2866 4001 4682 4873 826 Size: 219 1821 1135 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2058 2845 1872 Size: 186 787 4545 Fragments arranged by size: 4545 787 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2004 2106 2957 513 Size: 552 939 102 851 3074 Fragments arranged by size: 3074 939 851 552 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 2281 2698 3545 3562 3715 3900 Size: 1255 560 141 417 847 17 153 185 Cuts at: 3900 4345 325 Size: 445 1498 Fragments arranged by size: 1498 1255 847 560 445 417 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2056 2843 1870 Size: 186 787 4545 Fragments arranged by size: 4545 787 186 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI