Restriction map for
pFN21AE4573
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BanI
BbeI BcgI BciVI BclI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SwaI Tru9I XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AclI 1084 AACGTT AA^CGTT
AflIII 834 ACGTGT A^C[GA][CT]GT
AhdI 760 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1437 CCTCAGG CC^T[ACGT]AGG
AspEI 760 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1741 GTGCTC G[AT]GC[AT]^C
AspI 340 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 256 GGATCC G^GATCC
BbrPI 1107 CACGTG CAC^GTG
BbvCI 1101 CCTCAGC CCTCAGC(-5/-2)
BsaI 634 GAGACC GGTCTC(1/5)
BsgI 1073 GTGCAG GTGCAG(16/14)
BsiHKAI 1741 GTGCTC G[AT]GC[AT]^C
BspHI 1254 TCATGA T^CATGA
BsrDI 1952 GCAATG GCAATG(2/0)
BssSI 1534 CACGAG CACGAG(-5/-1)
Bsu36I 1437 CCTCAGG CC^T[ACGT]AGG
DraIII 1177 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 88 CCATGG C^C[GA][CT]GG
Eam1105I 760 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1437 CCTCAGG CC^T[ACGT]AGG
EcoT22I 2041 ATGCAT ATGCA^T
FauI 1723 GCGGG CCCGC(4/6)
NcoI 88 CCATGG C^CATGG
NdeI 126 CATATG CA^TATG
NheI 2119 GCTAGC G^CTAGC
NsiI 2041 ATGCAT ATGCA^T
PflFI 340 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1107 CACGTG CAC^GTG
PmlI 1107 CACGTG CAC^GTG
Psp1406I 1084 AACGTT AA^CGTT
RcaI 1254 TCATGA T^CATGA
SapI 590 GCTCTTC GCTCTTC(1/4)
SbfI 309 CCTGCAGG CCTGCA^GG
SmaI 1447 CCCGGG CCC^GGG
SmlI 1895 CTCAAG C^T[CT][GA]AG
Sse8387I 309 CCTGCAGG CCTGCA^GG
SspI 2170 AATATT AAT^ATT
TaqI 1116 TCGA T^CGA
Tth111I 340 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1116 TCGA T^CGA
XbaI 867 TCTAGA T^CTAGA
XcmI 1377 CCAGCACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1447 CCCGGG C^CCGGG
XmaI 1447 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 1718 CCGC CCGC(-3/-1)
1723 GCGG
AlwNI 224 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
944 CAGGGACTG
AvaI 251 CCCGAG C^[CT]CG[GA]G
1447 CCCGGG
BbsI 757 GAAGAC GAAGAC(2/6)
2088 GAAGAC
BcnI 1447 CCCGG CC^[GC]GG
1448 CCGGG
BglII 823 AGATCT A^GATCT
1991 AGATCT
BlpI 1280 GCTCAGC GC^T[ACGT]AGC
1743 GCTCAGC
Bpu1102I 1280 GCTCAGC GC^T[ACGT]AGC
1743 GCTCAGC
BpuAI 757 GAAGAC GAAGAC(2/6)
2088 GAAGAC
BsaAI 833 TACGTG [CT]AC^GT[GA]
1107 CACGTG
BsoBI 251 CCCGAG C^[CT]CG[GA]G
1447 CCCGGG
Bsp1407I 837 TGTACA T^GTACA
1946 TGTACA
BsrGI 837 TGTACA T^GTACA
1946 TGTACA
CelII 1280 GCTCAGC GC^T[ACGT]AGC
1743 GCTCAGC
EcoNI 150 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1868 CCTCGTCCAGG
EcoRI 453 GAATTC G^AATTC
1130 GAATTC
EcoT14I 88 CCATGG C^C[AT][AT]GG
1547 CCTTGG
NciI 1447 CCCGG CC^[GC]GG
1448 CCGGG
NspI 279 GCATGT [GA]CATG^[CT]
1029 GCATGT
PflMI 1176 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1455 CCAGGCTGTGG
SspBI 837 TGTACA T^GTACA
1946 TGTACA
StuI 1710 AGGCCT AGG^CCT
1876 AGGCCT
StyI 88 CCATGG C^C[AT][AT]GG
1547 CCTTGG
Van91I 1176 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1455 CCAGGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AcsI AfaI AluI AlwI ApaI
ApoI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp45I Tsp509I TspRI XhoII