Restriction map for
pFN21AE4573
(Circular) (Six-base) MAPSORT of: pFN21AE4573.seq Check: 102 from: 1 to: 6931
pFN21AE4573 6931 bp
With 182 enzymes: SgfI *
March 28, 2012 14:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4741 278
Size: 53 83 186 936 3205 2468
Fragments arranged by size:
3205 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4274 1937
Size: 2337 4594
Acc65I G'GTAC_C
Cuts at: 4252 4252
Size: 6931
AclI AA'CG_TT
Cuts at: 1563 3090 5058 5431 1563
Size: 1527 1968 373 3063
Fragments arranged by size:
3063 1968 1527 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2674 2781 3148 4109 4994 6378 6646 1632
Size: 1042 107 367 961 885 1384 268 1917
Fragments arranged by size:
1917 1384 1042 961 885 367 268 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6734
AflIII A'CryG_T
Cuts at: 1203 2839 5830 6807 1203
Size: 1636 2991 977 1327
Fragments arranged by size:
2991 1636 1327 977
AgeI A'CCGG_T
Cuts at: 5762 5762
Size: 6931
AhdI GACnn_n'nnGTC
Cuts at: 2770 5660 2770
Size: 2890 4041
AleI CACnn'nnGTG
Cuts at: 1166 3188 1166
Size: 2022 4909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4056 4088 4056
Size: 32 6899
AlwNI CAG_nnn'CTG
Cuts at: 2234 2954 4674 6246 2234
Size: 720 1720 1572 2919
Fragments arranged by size:
2919 1720 1572 720
ApaI G_GGCC'C
Cuts at: 3181 3382 3460 3661 3802 4144 3181
Size: 201 78 201 141 342 5968
Fragments arranged by size:
5968 342 201 201 141 78
ApaLI G'TGCA_C
Cuts at: 4988 6144 4988
Size: 1156 5775
ApoI r'AATT_y
Cuts at: 1493 2291 2458 3135 4240 4466 4618 6727 1493
Size: 798 167 677 1105 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 798 677 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 5485 160
Size: 872 4453 1606
Fragments arranged by size:
4453 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6931
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 3452 4256 1870
Size: 73 313 1196 804 4545
Fragments arranged by size:
4545 1196 804 313 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3061 3094 3061
Size: 33 6898
BamHI G'GATC_C
Cuts at: 2261 4261 4628 2261
Size: 2000 367 4564
Fragments arranged by size:
4564 2000 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4252 5707 618
Size: 325 217 627 2465 1455 1842
Fragments arranged by size:
2465 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3172 3181 3382 3451 3460 3661 3802 3909
Size: 1781 9 201 69 9 201 141 107
Cuts at: 3909 4144 4250 1391
Size: 235 106 4072
Fragments arranged by size:
4072 1781 235 201 201 141 107 106
69 9 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 4100 928
Size: 971 870 1331 3759
Fragments arranged by size:
3759 1331 971 870
BbvCI CC'TCA_GC
Cuts at: 3107 3107
Size: 6931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5122 5156 1903
Size: 34 50 34 3101 34 3678
Fragments arranged by size:
3678 3101 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4823 6033 4823
Size: 1210 5721
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6931
BfrBI ATG'CAT
Cuts at: 4048 4048
Size: 6931
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5542 136
Size: 107 122 71 631 605 3870 1525
Fragments arranged by size:
3870 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2828 3996 6926 2828
Size: 1168 2930 2833
Fragments arranged by size:
2930 2833 1168
BlpI GC'TnA_GC
Cuts at: 3286 3749 4348 3286
Size: 463 599 5869
Fragments arranged by size:
5869 599 463
Bme1580I G_kGCm'C
Cuts at: 1790 3181 3382 3460 3661 3802 4144 4992 6148
Size: 1391 201 78 201 141 342 848 1156
Cuts at: 6148 1790
Size: 2573
Fragments arranged by size:
2573 1391 1156 848 342 201 201 141
78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6931
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4662 5620 442
Size: 416 53 83 399 24 3245 958 1753
Fragments arranged by size:
3245 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4128 1056
Size: 3072 3859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3249 3419 3889 3249
Size: 170 470 6291
Fragments arranged by size:
6291 470 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2452 2723 2740 3224 3318 3503 3597 3962
Size: 1213 271 17 484 94 185 94 365
Cuts at: 3962 3990 4021 5591 1239
Size: 28 31 1570 2579
Fragments arranged by size:
2579 1570 1213 484 365 271 185 94
94 31 28 17
Bpu10I CC'TnA_GC
Cuts at: 3107 3107
Size: 6931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3885 5056 5921 6219 3885
Size: 1171 865 298 4597
Fragments arranged by size:
4597 1171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 5594 882
Size: 617 1134 2961 2219
Fragments arranged by size:
2961 2219 1134 617
BsaAI yAC'GTr
Cuts at: 493 1204 2840 3114 4722 493
Size: 711 1636 274 1608 2702
Fragments arranged by size:
2702 1636 1608 711 274
BsaBI GATnn'nnATC
Cuts at: 6925 6925
Size: 6931
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4738 5120 5755 275
Size: 53 83 186 936 3205 382 635 1451
Fragments arranged by size:
3205 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2888 3139 3418 5364 5762 6036 6183 6594 2888
Size: 251 279 1946 398 274 147 411 3225
Fragments arranged by size:
3225 1946 411 398 279 274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2106 2136 2334 2364 3935
Size: 30 165 30 703 30 198 30 1571
Cuts at: 3935 3965 1178
Size: 30 4144
Fragments arranged by size:
4144 1571 703 198 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 3315 3318 3594 1186
Size: 886 1243 3 276 4523
Fragments arranged by size:
4523 1243 886 276 3
BseYI C'CCAG_C
Cuts at: 2643 3009 3381 3660 6134 6630 2643
Size: 366 372 279 2474 496 2944
Fragments arranged by size:
2944 2474 496 372 366 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3099 1163
Size: 573 1363 4995
Fragments arranged by size:
4995 1363 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4381 5142 6170 1540
Size: 275 186 2380 761 1028 2301
Fragments arranged by size:
2380 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 4992 5077 6148 3750
Size: 1242 85 1071 4533
Fragments arranged by size:
4533 1242 1071 85
BsmI GAATG_Cn'
Cuts at: 4442 4535 4442
Size: 93 6838
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3172 3181 3382 3451 3460 3661 3750
Size: 399 1382 9 201 69 9 201 89
Cuts at: 3750 3802 3909 4144 4250 4992 5077 6148 1391
Size: 52 107 235 106 742 85 1071 2174
Fragments arranged by size:
2174 1382 1071 742 399 235 201 201
107 106 89 85 69 52 9 9
BspEI T'CCGG_A
Cuts at: 6594 6594
Size: 6931
BspHI T'CATG_A
Cuts at: 3259 4820 6550 3259
Size: 1561 1730 3640
Fragments arranged by size:
3640 1730 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2715 3207 3486 4286 844
Size: 1871 492 279 800 3489
Fragments arranged by size:
3489 1871 800 492 279
BsrBI CCG'CTC
Cuts at: 1394 1823 4818 1394
Size: 429 2995 3507
Fragments arranged by size:
3507 2995 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3964 5426 5600 65
Size: 1183 2716 1462 174 1396
Fragments arranged by size:
2716 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5575 5762 1700
Size: 110 3765 187 2869
Fragments arranged by size:
3765 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2842 3951 96
Size: 2746 1109 3076
Fragments arranged by size:
3076 2746 1109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6931
BssSI C'ACGA_G
Cuts at: 3539 4991 6003 3539
Size: 1452 1012 4467
Fragments arranged by size:
4467 1452 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6931
BstBI TT'CG_AA
Cuts at: 5735 5735
Size: 6931
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6931
BstYI r'GATC_y
Cuts at: 1972 2217 2261 2742 2828 2877 3996 4261 4628
Size: 245 44 481 86 49 1119 265 367
Cuts at: 4628 5013 5030 6482 6568 6580 6926 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1119 481 385 367 346 265
245 86 86 49 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 3443 3443
Size: 6931
BtgI C'CryG_G
Cuts at: 513 1065 2093 513
Size: 552 1028 5351
Fragments arranged by size:
5351 1028 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6192
Fragments arranged by size:
6192 683 56
BtsI GCAGTG_nn'
Cuts at: 4447 5241 5261 4447
Size: 794 20 6117
Fragments arranged by size:
6117 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6931
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6896
DraI TTT'AAA
Cuts at: 4235 4235
Size: 6931
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 6931
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5938 809
Size: 507 4622 1802
Fragments arranged by size:
4622 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4378 5267 6896
Size: 54 1425 50 275 18 2548 889 1629
Cuts at: 6896 8
Size: 43
Fragments arranged by size:
2548 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4378 1537
Size: 275 2566 4090
Fragments arranged by size:
4090 2566 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2038 2602 2693 2977 4861 1353
Size: 685 564 91 284 1884 3423
Fragments arranged by size:
3423 1884 685 564 284 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5518 5892 6038 387
Size: 1064 138 3929 374 146 1280
Fragments arranged by size:
3929 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2452 2674 2723 2740 2781 3148 3224
Size: 393 820 222 49 17 41 367 76
Cuts at: 3224 3318 3503 3597 3962 3990 4021 4109 4994
Size: 94 185 94 365 28 31 88 885
Cuts at: 4994 5591 6378 6646 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 885 820 787 597 393 367 365
268 222 185 94 94 88 76 49
41 31 28 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 3877 2159
Size: 1718 5213
EcoO109I rG'GnC_Cy
Cuts at: 2727 3177 3196 3378 3456 3475 3657 3798 4140
Size: 450 19 182 78 19 182 141 342
Cuts at: 4140 2727
Size: 5518
Fragments arranged by size:
5518 450 342 182 182 141 78 19
19
EcoRI G'AATT_C
Cuts at: 2458 3135 4240 4618 2458
Size: 677 1105 378 4771
Fragments arranged by size:
4771 1105 677 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 6931
FspI TGC'GCA
Cuts at: 5437 5437
Size: 6931
HaeII r_GCGC'y
Cuts at: 1678 6078 1678
Size: 4400 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2253 2285 2334
Size: 32 135 32 448 32 201 32 49
Cuts at: 2334 2366 2727 2756 2759 2785 2788 2817 3883
Size: 32 361 29 3 26 3 29 1066
Cuts at: 3883 3915 5572 5604 5646 5678 1373
Size: 32 1657 32 42 32 2626
Fragments arranged by size:
2626 1657 1066 448 361 201 135 49
42 32 32 32 32 32 32 32
32 29 29 26 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4275 4521 669
Size: 988 281 2337 246 3079
Fragments arranged by size:
3079 2337 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6931
HpaI GTT'AAC
Cuts at: 4521 4521
Size: 6931
KpnI G_GTAC'C
Cuts at: 4256 4256
Size: 6931
MfeI C'AATT_G
Cuts at: 4530 4530
Size: 6931
MluI A'CGCG_T
Cuts at: 6807 6807
Size: 6931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6045 6229 1305
Size: 4740 184 2007
Fragments arranged by size:
4740 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5109
Fragments arranged by size:
5109 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3264 3390 3543 4889 5248 5407
Size: 648 2022 76 126 153 1346 359 159
Cuts at: 5407 518
Size: 2042
Fragments arranged by size:
2042 2022 1346 648 359 159 153 126
76
MspA1I CmG'CkG
Cuts at: 1606 2674 2899 3083 3725 4347 5024 6172 6417
Size: 1068 225 184 642 622 677 1148 245
Cuts at: 6417 1606
Size: 2120
Fragments arranged by size:
2120 1148 1068 677 642 622 245 225
184
NcoI C'CATG_G
Cuts at: 513 1065 2093 513
Size: 552 1028 5351
Fragments arranged by size:
5351 1028 552
NdeI CA'TA_TG
Cuts at: 387 2132 387
Size: 1745 5186
NheI G'CTAG_C
Cuts at: 1052 4124 1052
Size: 3072 3859
NotI GC'GGCC_GC
Cuts at: 4378 4378
Size: 6931
NsiI A_TGCA'T
Cuts at: 4050 4050
Size: 6931
NspI r_CATG'y
Cuts at: 1132 1229 2288 3038 4289 5834 1132
Size: 97 1059 750 1251 1545 2229
Fragments arranged by size:
2229 1545 1251 1059 750 97
PasI CC'CwG_GG
Cuts at: 1348 2161 2941 3174 3274 1348
Size: 813 780 233 100 5005
Fragments arranged by size:
5005 813 780 233 100
PciI A'CATG_T
Cuts at: 5830 5830
Size: 6931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3187 3466 1108
Size: 2079 279 4573
Fragments arranged by size:
4573 2079 279
PfoI T'CCnGG_A
Cuts at: 2038 3701 3887 3967 2038
Size: 1663 186 80 5002
Fragments arranged by size:
5002 1663 186 80
PmeI GTTT'AAAC
Cuts at: 4235 4235
Size: 6931
PmlI CAC'GTG
Cuts at: 3114 3114
Size: 6931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5000 5032 6513 6545 5000
Size: 32 1481 32 5386
Fragments arranged by size:
5386 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 3196 3475 2727
Size: 469 279 6183
Fragments arranged by size:
6183 469 279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6931
PsiI TTA'TAA
Cuts at: 2436 4501 2436
Size: 2065 4866
PspOMI G'GGCC_C
Cuts at: 3177 3378 3456 3657 3798 4140 3177
Size: 201 78 201 141 342 5968
Fragments arranged by size:
5968 342 201 201 141 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3703 3735 3703
Size: 32 6899
PstI C_TGCA'G
Cuts at: 830 2319 3334 4117 4283 830
Size: 1489 1015 783 166 3478
Fragments arranged by size:
3478 1489 1015 783 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6931
PvuII CAG'CTG
Cuts at: 2674 2899 3083 2674
Size: 225 184 6522
Fragments arranged by size:
6522 225 184
SalI G'TCGA_C
Cuts at: 1936 4273 1936
Size: 2337 4594
SapI GCTCTTCn'nnn_
Cuts at: 2602 2602
Size: 6931
SbfI CC_TGCA'GG
Cuts at: 2319 4283 2319
Size: 1964 4967
ScaI AGT'ACT
Cuts at: 5179 6891 5179
Size: 1712 5219
SfcI C'TryA_G
Cuts at: 826 1045 2315 3330 3609 4113 4279 5414 6095
Size: 219 1270 1015 279 504 166 1135 681
Cuts at: 6095 6286 826
Size: 191 1471
Fragments arranged by size:
1471 1270 1135 1015 681 504 279 219
191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6931
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6931
SmaI CCC'GGG
Cuts at: 1872 3454 4258 1872
Size: 1582 804 4545
Fragments arranged by size:
4545 1582 804
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3900 5035 5936 6198 820
Size: 197 926 1957 1135 901 262 1553
Fragments arranged by size:
1957 1553 1135 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4722 493
Size: 4229 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6931
SphI G_CATG'C
Cuts at: 1132 4289 1132
Size: 3157 3774
SspI AAT'ATT
Cuts at: 5 52 4177 4855 5
Size: 47 4125 678 2081
Fragments arranged by size:
4125 2081 678 47
StuI AGG'CCT
Cuts at: 1517 3717 3883 1517
Size: 2200 166 4565
Fragments arranged by size:
4565 2200 166
StyI C'CwwG_G
Cuts at: 513 1065 2093 3552 4370 513
Size: 552 1028 1459 818 3074
Fragments arranged by size:
3074 1459 1028 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4116 4958 4975 5128 5313 5758 325
Size: 1255 2536 842 17 153 185 445 1498
Fragments arranged by size:
2536 1498 1255 842 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 2492 2842
Size: 274 80 33 51 1443 97 418 350
Cuts at: 2842 3525 3951 5177 6889 96
Size: 683 426 1226 1712 138
Fragments arranged by size:
1712 1443 1226 683 426 418 350 274
138 97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2106 2138 3203 3235 3482 3514 6891 6923 2106
Size: 32 1065 32 247 32 3377 32 2114
Fragments arranged by size:
3377 2114 1065 247 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2348 1316
Size: 1032 5899
XbaI T'CTAG_A
Cuts at: 2872 4267 2872
Size: 1395 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3389 3389
Size: 6931
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6931
XmaI C'CCGG_G
Cuts at: 1870 3452 4256 1870
Size: 1582 804 4545
Fragments arranged by size:
4545 1582 804
XmnI GAAnn'nnTTC
Cuts at: 5060 5060
Size: 6931
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4739 276
Size: 53 83 186 936 3205 2468
Fragments arranged by size:
3205 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI