(Linear) (Six-base) MAPSORT of: KIEE4573.seq Check: 7802 from: 1 to: 2226 KIEE4573.orf With 182 enzymes: SgfI * March 22, 2012 18:25 .. AclI AA'CG_TT Cuts at: 0 1085 2226 Size: 1085 1141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 669 776 1143 2104 2226 Size: 669 107 367 961 122 Fragments arranged by size: 961 669 367 122 107 AflIII A'CryG_T Cuts at: 0 834 2226 Size: 834 1392 AhdI GACnn_n'nnGTC Cuts at: 0 765 2226 Size: 765 1461 AleI CACnn'nnGTG Cuts at: 0 1183 2226 Size: 1183 1043 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2051 2083 2226 Size: 2051 32 143 Fragments arranged by size: 2051 143 32 AlwNI CAG_nnn'CTG Cuts at: 0 229 949 2226 Size: 229 720 1277 Fragments arranged by size: 1277 720 229 ApaI G_GGCC'C Cuts at: 0 1176 1377 1455 1656 1797 2139 2226 Size: 1176 201 78 201 141 342 87 Fragments arranged by size: 1176 342 201 201 141 87 78 ApoI r'AATT_y Cuts at: 0 286 453 1130 2226 Size: 286 167 677 1096 Fragments arranged by size: 1096 677 286 167 AvaI C'yCGr_G Cuts at: 0 251 1447 2226 Size: 251 1196 779 Fragments arranged by size: 1196 779 251 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1056 1089 2226 Size: 1056 33 1137 Fragments arranged by size: 1137 1056 33 BamHI G'GATC_C Cuts at: 0 256 2226 Size: 256 1970 BanII G_rGCy'C Cuts at: 0 1167 1176 1377 1446 1455 1656 1797 1904 Size: 1167 9 201 69 9 201 141 107 Cuts at: 1904 2139 2226 Size: 235 87 Fragments arranged by size: 1167 235 201 201 141 107 87 69 9 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 764 2095 2226 Size: 764 1331 131 Fragments arranged by size: 1331 764 131 BbvCI CC'TCA_GC Cuts at: 0 1102 2226 Size: 1102 1124 BfrBI ATG'CAT Cuts at: 0 2043 2226 Size: 2043 183 BglII A'GATC_T Cuts at: 0 823 1991 2226 Size: 823 1168 235 Fragments arranged by size: 1168 823 235 BlpI GC'TnA_GC Cuts at: 0 1281 1744 2226 Size: 1281 463 482 Fragments arranged by size: 1281 482 463 Bme1580I G_kGCm'C Cuts at: 0 1176 1377 1455 1656 1797 2139 2226 Size: 1176 201 78 201 141 342 87 Fragments arranged by size: 1176 342 201 201 141 87 78 BmtI G_CTAG'C Cuts at: 0 2123 2226 Size: 2123 103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1244 1414 1884 2226 Size: 1244 170 470 342 Fragments arranged by size: 1244 470 342 170 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 718 735 1219 1313 1498 1592 1957 Size: 447 271 17 484 94 185 94 365 Cuts at: 1957 1985 2016 2226 Size: 28 31 210 Fragments arranged by size: 484 447 365 271 210 185 94 94 31 28 17 Bpu10I CC'TnA_GC Cuts at: 0 1102 2226 Size: 1102 1124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1880 2226 Size: 1880 346 BsaI GGTCTCn'nnnn_ Cuts at: 0 628 2226 Size: 628 1598 BsaAI yAC'GTr Cuts at: 0 835 1109 2226 Size: 835 274 1117 Fragments arranged by size: 1117 835 274 BsaWI w'CCGG_w Cuts at: 0 883 1134 1413 2226 Size: 883 251 279 813 Fragments arranged by size: 883 813 279 251 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 101 131 329 359 1930 1960 2226 Size: 101 30 198 30 1571 30 266 Fragments arranged by size: 1571 266 198 101 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 1310 1313 1589 2226 Size: 67 1243 3 276 637 Fragments arranged by size: 1243 637 276 67 3 BseYI C'CCAG_C Cuts at: 0 638 1004 1376 1655 2226 Size: 638 366 372 279 571 Fragments arranged by size: 638 571 372 366 279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1094 2226 Size: 1094 1132 BsiHKAI G_wGCw'C Cuts at: 0 1745 2226 Size: 1745 481 Bsp1286I G_dGCh'C Cuts at: 0 1167 1176 1377 1446 1455 1656 1745 1797 Size: 1167 9 201 69 9 201 89 52 Cuts at: 1797 1904 2139 2226 Size: 107 235 87 Fragments arranged by size: 1167 235 201 201 107 89 87 69 52 9 9 BspHI T'CATG_A Cuts at: 0 1254 2226 Size: 1254 972 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 710 1202 1481 2226 Size: 710 492 279 745 Fragments arranged by size: 745 710 492 279 BsrDI GCAATG_nn' Cuts at: 0 1959 2226 Size: 1959 267 BsrGI T'GTAC_A Cuts at: 0 837 1946 2226 Size: 837 1109 280 Fragments arranged by size: 1109 837 280 BssSI C'ACGA_G Cuts at: 0 1534 2226 Size: 1534 692 BstYI r'GATC_y Cuts at: 0 212 256 737 823 872 1991 2226 Size: 212 44 481 86 49 1119 235 Fragments arranged by size: 1119 481 235 212 86 49 44 Bsu36I CC'TnA_GG Cuts at: 0 1438 2226 Size: 1438 788 BtgI C'CryG_G Cuts at: 0 88 2226 Size: 88 2138 DraIII CAC_nnn'GTG Cuts at: 0 1182 2226 Size: 1182 1044 EarI CTCTTCn'nnn_ Cuts at: 0 33 597 688 972 2226 Size: 33 564 91 284 1254 Fragments arranged by size: 1254 564 284 91 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 669 718 735 776 1143 1219 1313 Size: 447 222 49 17 41 367 76 94 Cuts at: 1313 1498 1592 1957 1985 2016 2104 2226 Size: 185 94 365 28 31 88 122 Fragments arranged by size: 447 367 365 222 185 122 94 94 88 76 49 41 31 28 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 1872 2226 Size: 154 1718 354 Fragments arranged by size: 1718 354 154 EcoO109I rG'GnC_Cy Cuts at: 0 722 1172 1191 1373 1451 1470 1652 1793 Size: 722 450 19 182 78 19 182 141 Cuts at: 1793 2135 2226 Size: 342 91 Fragments arranged by size: 722 450 342 182 182 141 91 78 19 19 EcoRI G'AATT_C Cuts at: 0 453 1130 2226 Size: 453 677 1096 Fragments arranged by size: 1096 677 453 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 88 2226 Size: 88 2138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 15 47 248 280 329 361 722 751 Size: 15 32 201 32 49 32 361 29 Cuts at: 751 754 780 783 812 1878 1910 2226 Size: 3 26 3 29 1066 32 316 Fragments arranged by size: 1066 361 316 201 49 32 32 32 32 29 29 26 15 3 3 MslI CAynn'nnrTG Cuts at: 0 1183 1259 1385 1538 2226 Size: 1183 76 126 153 688 Fragments arranged by size: 1183 688 153 126 76 MspA1I CmG'CkG Cuts at: 0 669 894 1078 1720 2226 Size: 669 225 184 642 506 Fragments arranged by size: 669 642 506 225 184 NcoI C'CATG_G Cuts at: 0 88 2226 Size: 88 2138 NdeI CA'TA_TG Cuts at: 0 127 2226 Size: 127 2099 NheI G'CTAG_C Cuts at: 0 2119 2226 Size: 2119 107 NsiI A_TGCA'T Cuts at: 0 2045 2226 Size: 2045 181 NspI r_CATG'y Cuts at: 0 283 1033 2226 Size: 283 750 1193 Fragments arranged by size: 1193 750 283 PasI CC'CwG_GG Cuts at: 0 156 936 1169 1269 2226 Size: 156 780 233 100 957 Fragments arranged by size: 957 780 233 156 100 PflMI CCAn_nnn'nTGG Cuts at: 0 1182 1461 2226 Size: 1182 279 765 Fragments arranged by size: 1182 765 279 PfoI T'CCnGG_A Cuts at: 0 33 1696 1882 1962 2226 Size: 33 1663 186 80 264 Fragments arranged by size: 1663 264 186 80 33 PmlI CAC'GTG Cuts at: 0 1109 2226 Size: 1109 1117 PpuMI rG'GwC_Cy Cuts at: 0 722 1191 1470 2226 Size: 722 469 279 756 Fragments arranged by size: 756 722 469 279 PsiI TTA'TAA Cuts at: 0 431 2226 Size: 431 1795 PspOMI G'GGCC_C Cuts at: 0 1172 1373 1451 1652 1793 2135 2226 Size: 1172 201 78 201 141 342 91 Fragments arranged by size: 1172 342 201 201 141 91 78 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1698 1730 2226 Size: 1698 32 496 Fragments arranged by size: 1698 496 32 PstI C_TGCA'G Cuts at: 0 314 1329 2112 2226 Size: 314 1015 783 114 Fragments arranged by size: 1015 783 314 114 PvuII CAG'CTG Cuts at: 0 669 894 1078 2226 Size: 669 225 184 1148 Fragments arranged by size: 1148 669 225 184 SapI GCTCTTCn'nnn_ Cuts at: 0 597 2226 Size: 597 1629 SbfI CC_TGCA'GG Cuts at: 0 314 2226 Size: 314 1912 SfcI C'TryA_G Cuts at: 0 310 1325 1604 2108 2226 Size: 310 1015 279 504 118 Fragments arranged by size: 1015 504 310 279 118 SmaI CCC'GGG Cuts at: 0 1449 2226 Size: 1449 777 SmlI C'TyrA_G Cuts at: 0 1895 2226 Size: 1895 331 SspI AAT'ATT Cuts at: 0 2172 2226 Size: 2172 54 StuI AGG'CCT Cuts at: 0 1712 1878 2226 Size: 1712 166 348 Fragments arranged by size: 1712 348 166 StyI C'CwwG_G Cuts at: 0 88 1547 2226 Size: 88 1459 679 Fragments arranged by size: 1459 679 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2111 2226 Size: 2111 115 TatI w'GTAC_w Cuts at: 0 69 487 837 1520 1946 2226 Size: 69 418 350 683 426 280 Fragments arranged by size: 683 426 418 350 280 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 101 133 1198 1230 1477 1509 2226 Size: 101 32 1065 32 247 32 717 Fragments arranged by size: 1065 717 247 101 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 343 2226 Size: 343 1883 XbaI T'CTAG_A Cuts at: 0 867 2226 Size: 867 1359 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1384 2226 Size: 1384 842 XmaI C'CCGG_G Cuts at: 0 1447 2226 Size: 1447 779 Enzymes that do cut: AclI AcuI AflIII AhdI AleI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstYI Bsu36I BtgI DraIII EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI PstI PvuII SapI SbfI SfcI SmaI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AlfI ApaLI AscI AseI AsiSI AvrII BanI BbeI BcgI BciVI BclI BglI BmgBI BmrI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV NotI NruI PacI PciI PmeI PpiI PshAI PspXI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XhoI XmnI ZraI