################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI Tsp45I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1247 GAATTC [GA]^AATT[CT] AcyI 637 GGCGTC G[GA]^CG[CT]C Aor51HI 18 AGCGCT AGC^GCT ApaI 236 GGGCCC GGGCC^C ApoI 1247 GAATTC [GA]^AATT[CT] AspI 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 959 TGCGCA TGC^GCA BanI 819 GGTGCC G^G[CT][GA]CC BcgI 140 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 1008 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 637 GGCGTC G[GA]^CG[CT]C BsgI 340 CTGCAC GTGCAG(16/14) BsmI 1109 GCATTC GAATGC(1/-1) BspMI 713 ACCTGC ACCTGC(4/8) BsrFI 355 ACCGGC [GA]^CCGG[CT] BssHII 386 GCGCGC G^CGCGC BstF5I 1007 GGATG GGATG(2/0) Cfr10I 355 ACCGGC [GA]^CCGG[CT] DraIII 1017 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 357 CGGCCG C^GGCCG EarI 866 CTCTTC CTCTTC(1/4) EclXI 357 CGGCCG C^GGCCG Eco47III 18 AGCGCT AGC^GCT Eco52I 357 CGGCCG C^GGCCG EcoRI 1247 GAATTC G^AATTC FokI 1007 GGATG GGATG(9/13) FspI 959 TGCGCA TGC^GCA HgaI 638 GCGTC GACGC(5/10) Hin1I 637 GGCGTC G[GA]^CG[CT]C HindIII 1357 AAGCTT A^AGCTT HpaI 1396 GTTAAC GTT^AAC Ksp632I 866 CTCTTC CTCTTC(1/4) MaeIII 1025 GTTAC ^GT[ACGT]AC MamI 1008 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflFI 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 558 AGGACCT [GA]G^G[AT]CC[CT] PshAI 796 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1284 CGATCG CGAT^CG SacI 430 GAGCTC GAGCT^C SbfI 399 CCTGCAGG CCTGCA^GG SmaI 232 CCCGGG CCC^GGG SphI 962 GCATGC GCATG^C Sse8387I 399 CCTGCAGG CCTGCA^GG Tsp509I 1248 AATT ^AATT Tth111I 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 232 CCCGGG C^CCGGG XmaI 232 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 188 CGCG CG^CG 387 CGCG AlwI 408 GATCC GGATC(4/5) 610 GGATC AspHI 151 GTGCTC G[AT]GC[AT]^C 430 GAGCTC AvaI 109 CTCGGG C^[CT]CG[GA]G 232 CCCGGG AvaII 559 GGACC G^G[AT]CC 711 GGACC BbsI 514 GAAGAC GAAGAC(2/6) 73 GTCTTC BfaI 68 CTAG C^TAG 1469 CTAG BlpI 432 GCTCAGC GC^T[ACGT]AGC 1099 GCTGAGC Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC 1099 GCTGAGC BpuAI 514 GAAGAC GAAGAC(2/6) 73 GTCTTC BsiHKAI 151 GTGCTC G[AT]GC[AT]^C 430 GAGCTC BsoBI 109 CTCGGG C^[CT]CG[GA]G 232 CCCGGG BspDI 424 ATCGAT AT^CGAT 439 ATCGAT BstUI 188 CGCG CG^CG 387 CGCG BstXI 254 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 281 CCAACCCCGTGG BstYI 407 AGATCC [GA]^GATC[CT] 610 GGATCT CelII 432 GCTCAGC GC^T[ACGT]AGC 1099 GCTGAGC ClaI 424 ATCGAT AT^CGAT 439 ATCGAT EcoNI 446 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 810 CCTAAGAAAGG MaeI 68 CTAG C^TAG 1469 CTAG MaeII 131 ACGT A^CGT 917 ACGT MflI 407 AGATCC [GA]^GATC[CT] 610 GGATCT MvnI 188 CGCG CG^CG 387 CGCG NspI 366 GCATGT [GA]CATG^[CT] 962 GCATGC PleI 28 GACTC GAGTC(4/5) 1381 GACTC SmlI 1318 CTCAAG C^T[CT][GA]AG 1401 CTTGAG TfiI 442 GATTC G^A[AT]TC 1067 GAATC XhoII 407 AGATCC [GA]^GATC[CT] 610 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwNI BanII BbvI BcnI BmyI BpmI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StyI TaqI Tru9I TseI TspRI TthHB8I XcmI