(Circular) (Six-base) MAPSORT of: pFN21AE3929.seq Check: 6000 from: 1 to: 6205 pFN21AE3929 6205 bp With 182 enzymes: SgfI * March 28, 2012 11:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2455 4268 5652 5920 1632 Size: 823 1813 1384 268 1917 Fragments arranged by size: 1917 1813 1384 823 268 AfeI AGC'GCT Cuts at: 2025 2025 Size: 6205 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 1184 Size: 858 5347 AlwNI CAG_nnn'CTG Cuts at: 2173 2728 2914 3948 5520 2173 Size: 555 186 1034 1572 2858 Fragments arranged by size: 2858 1572 1034 555 186 ApaI G_GGCC'C Cuts at: 2245 2245 Size: 6205 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 3252 3514 3740 3892 6001 1493 Size: 1759 262 226 152 2109 1697 Fragments arranged by size: 2109 1759 1697 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2237 3530 1870 Size: 73 171 123 1293 4545 Fragments arranged by size: 4545 1293 171 123 73 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2824 3526 4981 618 Size: 325 217 627 1037 702 1455 1842 Fragments arranged by size: 1842 1455 1037 702 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2106 2245 2439 2962 3524 1391 Size: 681 34 139 194 523 562 4072 Fragments arranged by size: 4072 681 562 523 194 139 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 2526 928 Size: 971 172 455 4607 Fragments arranged by size: 4607 971 455 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2134 2168 4396 4430 1903 Size: 34 50 34 113 34 2228 34 3678 Fragments arranged by size: 3678 2228 113 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 2438 3105 3622 2438 Size: 667 517 5021 Fragments arranged by size: 5021 667 517 Bme1580I G_kGCm'C Cuts at: 1790 2245 2259 4266 5422 1790 Size: 455 14 2007 1156 2573 Fragments arranged by size: 2573 2007 1156 455 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2431 3019 3936 Size: 416 53 83 399 24 1014 588 917 Cuts at: 3936 4894 442 Size: 958 1753 Fragments arranged by size: 1753 1014 958 917 588 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2624 2759 2936 4865 1239 Size: 1049 336 135 177 1929 2579 Fragments arranged by size: 2579 1929 1049 336 177 135 Bpu10I CC'TnA_GC Cuts at: 2954 2954 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3308 3427 4330 5195 5493 3308 Size: 119 903 865 298 4020 Fragments arranged by size: 4020 903 865 298 119 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 3017 6199 3017 Size: 3182 3023 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 4012 4394 5029 Size: 53 83 186 936 1110 1369 382 635 Cuts at: 5029 275 Size: 1451 Fragments arranged by size: 1451 1369 1110 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2372 2402 2432 Size: 30 165 30 633 30 306 30 30 Cuts at: 2432 2732 2762 1178 Size: 300 30 4621 Fragments arranged by size: 4621 633 306 300 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6205 BseYI C'CCAG_C Cuts at: 2258 2977 5408 5904 2258 Size: 719 2431 496 2559 Fragments arranged by size: 2559 2431 719 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 1163 Size: 573 594 5038 Fragments arranged by size: 5038 594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2317 2365 3292 3655 4416 5444 Size: 275 186 316 48 927 363 761 1028 Cuts at: 5444 1540 Size: 2301 Fragments arranged by size: 2301 1028 927 761 363 316 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2160 2439 4266 4351 5422 2160 Size: 279 1827 85 1071 2943 Fragments arranged by size: 2943 1827 1071 279 85 BsmI GAATG_Cn' Cuts at: 3114 3716 3809 3114 Size: 602 93 5510 Fragments arranged by size: 5510 602 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2106 2160 2245 2259 2439 2962 Size: 399 282 34 54 85 14 180 523 Cuts at: 2962 3524 4266 4351 5422 1391 Size: 562 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 523 399 282 180 85 85 54 34 14 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 3560 844 Size: 1883 833 3489 Fragments arranged by size: 3489 1883 833 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2360 4849 5036 1700 Size: 110 550 2489 187 2869 Fragments arranged by size: 2869 2489 550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 2391 1925 Size: 466 5739 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2293 2266 Size: 27 6178 BstYI r'GATC_y Cuts at: 1972 2412 2615 3535 3902 4287 4304 5756 5842 Size: 440 203 920 367 385 17 1452 86 Cuts at: 5842 5854 6200 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 920 440 385 367 346 203 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2211 2292 3066 513 Size: 552 1146 81 774 3652 Fragments arranged by size: 3652 1146 774 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 2430 2445 1554 Size: 876 15 5314 Fragments arranged by size: 5314 876 15 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 3027 3027 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2247 2362 Size: 54 1425 50 275 18 378 39 115 Cuts at: 2362 3652 4541 6170 8 Size: 1290 889 1629 43 Fragments arranged by size: 1629 1425 1290 889 378 275 115 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2362 3652 1537 Size: 275 550 1290 4090 Fragments arranged by size: 4090 1290 550 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2877 4135 1353 Size: 1524 1258 3423 Fragments arranged by size: 3423 1524 1258 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2437 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2455 2624 2759 2936 4268 4865 Size: 393 656 167 169 135 177 1332 597 Cuts at: 4865 5652 5920 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1332 787 656 597 393 268 177 169 167 135 EcoNI CCTnn'n_nnAGG Cuts at: 2455 2819 2455 Size: 364 5841 EcoO109I rG'GnC_Cy Cuts at: 2241 2242 2564 2241 Size: 1 322 5882 Fragments arranged by size: 5882 322 1 EcoRI G'AATT_C Cuts at: 3252 3514 3892 3252 Size: 262 378 5565 Fragments arranged by size: 5565 378 262 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3159 3159 Size: 6205 FspI TGC'GCA Cuts at: 2966 4711 2966 Size: 1745 4460 HaeII r_GCGC'y Cuts at: 1678 2027 2114 2174 5352 1678 Size: 349 87 60 3178 2531 Fragments arranged by size: 3178 2531 349 87 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2306 2338 3281 Size: 32 135 32 464 32 238 32 943 Cuts at: 3281 3313 4846 4878 4920 4952 1373 Size: 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 943 464 238 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 2534 3403 3549 3795 669 Size: 988 281 196 400 869 146 246 3079 Fragments arranged by size: 3079 988 869 400 281 246 196 146 HindIII A'AGCT_T Cuts at: 748 3362 748 Size: 2614 3591 HpaI GTT'AAC Cuts at: 3403 3795 3403 Size: 392 5813 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5319 5503 1305 Size: 4014 184 2007 Fragments arranged by size: 4014 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4383 Fragments arranged by size: 4383 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 2681 3154 3170 3437 4163 4522 Size: 648 1375 140 473 16 267 726 359 Cuts at: 4522 4681 518 Size: 159 2042 Fragments arranged by size: 2042 1375 726 648 473 359 267 159 140 16 MspA1I CmG'CkG Cuts at: 1606 2016 2041 2049 2386 2627 2699 2725 3335 Size: 410 25 8 337 241 72 26 610 Cuts at: 3335 3483 3621 4298 5446 5691 1606 Size: 148 138 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 610 410 337 245 241 148 138 72 26 25 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5653 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2375 2971 3563 5108 1132 Size: 97 1146 596 592 1545 2229 Fragments arranged by size: 2229 1545 1146 596 592 97 PasI CC'CwG_GG Cuts at: 1348 2065 1348 Size: 717 5488 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PfoI T'CCnGG_A Cuts at: 2601 2736 2601 Size: 135 6070 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2564 2564 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 2805 1613 Size: 1192 5013 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 2241 2241 Size: 6205 PstI C_TGCA'G Cuts at: 830 2087 2409 2459 2724 3557 830 Size: 1257 322 50 265 833 3478 Fragments arranged by size: 3478 1257 833 322 265 50 PvuI CG_AT'CG Cuts at: 2001 3292 2001 Size: 1291 4914 PvuII CAG'CTG Cuts at: 2386 2627 2699 2725 3335 2386 Size: 241 72 26 610 5256 Fragments arranged by size: 5256 610 241 72 26 SacI G_AGCT'C Cuts at: 2439 2439 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 2409 3557 2409 Size: 1148 5057 ScaI AGT'ACT Cuts at: 2306 2420 3472 4453 6165 2306 Size: 114 1052 981 1712 2346 Fragments arranged by size: 2346 1712 1052 981 114 SfcI C'TryA_G Cuts at: 826 1045 2083 2405 2455 2720 3090 3553 4688 Size: 219 1038 322 50 265 370 463 1135 Cuts at: 4688 5369 5560 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1038 681 463 370 322 265 219 191 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 2239 3532 1872 Size: 367 1293 4545 Fragments arranged by size: 4545 1293 367 SmlI C'TyrA_G Cuts at: 820 1017 1943 3323 3406 4309 5210 5472 820 Size: 197 926 1380 83 903 901 262 1553 Fragments arranged by size: 1553 1380 926 903 901 262 197 83 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 2971 3563 1132 Size: 1839 592 3774 Fragments arranged by size: 3774 1839 592 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2106 2197 2560 3007 3644 513 Size: 552 1041 91 363 447 637 3074 Fragments arranged by size: 3074 1041 637 552 447 363 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3038 4232 4249 4402 4587 5032 325 Size: 1255 1458 1194 17 153 185 445 1498 Fragments arranged by size: 1498 1458 1255 1194 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2418 3470 Size: 274 80 33 51 1443 327 114 1052 Cuts at: 3470 4451 6163 96 Size: 981 1712 138 Fragments arranged by size: 1712 1443 1052 981 327 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2732 2764 6165 6197 2372 Size: 32 328 32 3401 32 2380 Fragments arranged by size: 3401 2380 328 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2568 1316 Size: 1252 4953 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 2680 2911 2659 Size: 21 231 5953 Fragments arranged by size: 5953 231 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 2237 3530 1870 Size: 367 1293 4545 Fragments arranged by size: 4545 1293 367 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI