(Linear) (Six-base) MAPSORT of: KIEE3929.seq Check: 6065 from: 1 to: 1500 KIEE3929.orf With 182 enzymes: SgfI * March 22, 2012 16:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 450 1500 Size: 450 1050 AfeI AGC'GCT Cuts at: 0 20 1500 Size: 20 1480 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 37 1500 Size: 37 1463 AlwNI CAG_nnn'CTG Cuts at: 0 168 723 909 1500 Size: 168 555 186 591 Fragments arranged by size: 591 555 186 168 ApaI G_GGCC'C Cuts at: 0 240 1500 Size: 240 1260 ApoI r'AATT_y Cuts at: 0 1247 1500 Size: 1247 253 AvaI C'yCGr_G Cuts at: 0 109 232 1500 Size: 109 123 1268 Fragments arranged by size: 1268 123 109 BanI G'GyrC_C Cuts at: 0 819 1500 Size: 819 681 BanII G_rGCy'C Cuts at: 0 67 101 240 434 957 1500 Size: 67 34 139 194 523 543 Fragments arranged by size: 543 523 194 139 67 34 BbsI GAAGACnn'nnnn_ Cuts at: 0 66 521 1500 Size: 66 455 979 Fragments arranged by size: 979 455 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 129 163 1500 Size: 129 34 1337 Fragments arranged by size: 1337 129 34 BlpI GC'TnA_GC Cuts at: 0 433 1100 1500 Size: 433 667 400 Fragments arranged by size: 667 433 400 Bme1580I G_kGCm'C Cuts at: 0 240 254 1500 Size: 240 14 1246 Fragments arranged by size: 1246 240 14 BmrI ACTGGGnnnn_n' Cuts at: 0 426 1014 1500 Size: 426 588 486 Fragments arranged by size: 588 486 426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 619 754 931 1500 Size: 283 336 135 177 569 Fragments arranged by size: 569 336 283 177 135 Bpu10I CC'TnA_GC Cuts at: 0 949 1500 Size: 949 551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1303 1422 1500 Size: 1303 119 78 Fragments arranged by size: 1303 119 78 BsaBI GATnn'nnATC Cuts at: 0 1012 1500 Size: 1012 488 BsaHI Gr'CG_yC Cuts at: 0 638 1500 Size: 638 862 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 31 61 367 397 427 727 757 1500 Size: 31 30 306 30 30 300 30 743 Fragments arranged by size: 743 306 300 31 30 30 30 30 BseYI C'CCAG_C Cuts at: 0 253 972 1500 Size: 253 719 528 Fragments arranged by size: 719 528 253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 1500 Size: 325 1175 BsiEI CG_ry'CG Cuts at: 0 312 360 1287 1500 Size: 312 48 927 213 Fragments arranged by size: 927 312 213 48 BsiHKAI G_wGCw'C Cuts at: 0 155 434 1500 Size: 155 279 1066 Fragments arranged by size: 1066 279 155 BsmI GAATG_Cn' Cuts at: 0 1109 1500 Size: 1109 391 Bsp1286I G_dGCh'C Cuts at: 0 67 101 155 240 254 434 957 1500 Size: 67 34 54 85 14 180 523 543 Fragments arranged by size: 543 523 180 85 67 54 34 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 722 1500 Size: 722 778 BsrFI r'CCGG_y Cuts at: 0 355 1500 Size: 355 1145 BssHII G'CGCG_C Cuts at: 0 386 1500 Size: 386 1114 BstXI CCAn_nnnn'nTGG Cuts at: 0 261 288 1500 Size: 261 27 1212 Fragments arranged by size: 1212 261 27 BstYI r'GATC_y Cuts at: 0 407 610 1500 Size: 407 203 890 Fragments arranged by size: 890 407 203 BtgI C'CryG_G Cuts at: 0 206 287 1061 1500 Size: 206 81 774 439 Fragments arranged by size: 774 439 206 81 ClaI AT'CG_AT Cuts at: 0 425 440 1500 Size: 425 15 1060 Fragments arranged by size: 1060 425 15 DraIII CAC_nnn'GTG Cuts at: 0 1022 1500 Size: 1022 478 EaeI y'GGCC_r Cuts at: 0 203 242 357 1500 Size: 203 39 115 1143 Fragments arranged by size: 1143 203 115 39 EagI C'GGCC_G Cuts at: 0 357 1500 Size: 357 1143 EarI CTCTTCn'nnn_ Cuts at: 0 872 1500 Size: 872 628 EcoICRI GAG'CTC Cuts at: 0 432 1500 Size: 432 1068 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 450 619 754 931 1500 Size: 283 167 169 135 177 569 Fragments arranged by size: 569 283 177 169 167 135 EcoNI CCTnn'n_nnAGG Cuts at: 0 450 814 1500 Size: 450 364 686 Fragments arranged by size: 686 450 364 EcoO109I rG'GnC_Cy Cuts at: 0 236 237 559 1500 Size: 236 1 322 941 Fragments arranged by size: 941 322 236 1 EcoRI G'AATT_C Cuts at: 0 1247 1500 Size: 1247 253 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1154 1500 Size: 1154 346 FspI TGC'GCA Cuts at: 0 961 1500 Size: 961 539 HaeII r_GCGC'y Cuts at: 0 22 109 169 1500 Size: 22 87 60 1331 Fragments arranged by size: 1331 87 60 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 31 63 301 333 1276 1308 1500 Size: 31 32 238 32 943 32 192 Fragments arranged by size: 943 238 192 32 32 32 31 HincII GTy'rAC Cuts at: 0 129 529 1398 1500 Size: 129 400 869 102 Fragments arranged by size: 869 400 129 102 HindIII A'AGCT_T Cuts at: 0 1357 1500 Size: 1357 143 HpaI GTT'AAC Cuts at: 0 1398 1500 Size: 1398 102 MslI CAynn'nnrTG Cuts at: 0 536 676 1149 1165 1432 1500 Size: 536 140 473 16 267 68 Fragments arranged by size: 536 473 267 140 68 16 MspA1I CmG'CkG Cuts at: 0 11 36 44 381 622 694 720 1330 Size: 11 25 8 337 241 72 26 610 Cuts at: 1330 1478 1500 Size: 148 22 Fragments arranged by size: 610 337 241 148 72 26 25 22 11 8 NspI r_CATG'y Cuts at: 0 370 966 1500 Size: 370 596 534 Fragments arranged by size: 596 534 370 PasI CC'CwG_GG Cuts at: 0 60 1500 Size: 60 1440 PfoI T'CCnGG_A Cuts at: 0 596 731 1500 Size: 596 135 769 Fragments arranged by size: 769 596 135 PpuMI rG'GwC_Cy Cuts at: 0 559 1500 Size: 559 941 PshAI GACnn'nnGTC Cuts at: 0 800 1500 Size: 800 700 PspOMI G'GGCC_C Cuts at: 0 236 1500 Size: 236 1264 PstI C_TGCA'G Cuts at: 0 82 404 454 719 1500 Size: 82 322 50 265 781 Fragments arranged by size: 781 322 265 82 50 PvuI CG_AT'CG Cuts at: 0 1287 1500 Size: 1287 213 PvuII CAG'CTG Cuts at: 0 381 622 694 720 1330 1500 Size: 381 241 72 26 610 170 Fragments arranged by size: 610 381 241 170 72 26 SacI G_AGCT'C Cuts at: 0 434 1500 Size: 434 1066 SbfI CC_TGCA'GG Cuts at: 0 404 1500 Size: 404 1096 ScaI AGT'ACT Cuts at: 0 301 415 1467 1500 Size: 301 114 1052 33 Fragments arranged by size: 1052 301 114 33 SfcI C'TryA_G Cuts at: 0 78 400 450 715 1085 1500 Size: 78 322 50 265 370 415 Fragments arranged by size: 415 370 322 265 78 50 SmaI CCC'GGG Cuts at: 0 234 1500 Size: 234 1266 SmlI C'TyrA_G Cuts at: 0 1318 1401 1500 Size: 1318 83 99 Fragments arranged by size: 1318 99 83 SphI G_CATG'C Cuts at: 0 966 1500 Size: 966 534 StyI C'CwwG_G Cuts at: 0 101 192 555 1002 1500 Size: 101 91 363 447 498 Fragments arranged by size: 498 447 363 101 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1033 1500 Size: 1033 467 TatI w'GTAC_w Cuts at: 0 299 413 1465 1500 Size: 299 114 1052 35 Fragments arranged by size: 1052 299 114 35 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 367 399 727 759 1500 Size: 367 32 328 32 741 Fragments arranged by size: 741 367 328 32 32 Tth111I GACn'n_nGTC Cuts at: 0 563 1500 Size: 563 937 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 654 675 906 1500 Size: 654 21 231 594 Fragments arranged by size: 654 594 231 21 XmaI C'CCGG_G Cuts at: 0 232 1500 Size: 232 1268 Enzymes that do cut: AcuI AfeI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII BstXI BstYI BtgI ClaI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI MslI MspA1I NspI PasI PfoI PpuMI PshAI PspOMI PstI PvuI PvuII SacI SbfI ScaI SfcI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaWI BseRI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI EciI EcoRV FseI FspAI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI RsrII SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI