################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AflII AgeI AhdI ApaI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI FauI FbaI FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacII SalI ScaI SfiI SfoI SgrAI SmaI SphI StuI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 3205 GTCTAC GT^[AC][GT]AC Alw44I 1076 GTGCAC G^TGCAC Aor51HI 2680 AGCGCT AGC^GCT ApaLI 1076 GTGCAC G^TGCAC AspI 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 876 TGGCCA TGG^CCA BamHI 113 GGATCC G^GATCC BanI 2010 GGTGCC G^G[CT][GA]CC BbsI 799 GAAGAC GAAGAC(2/6) BciVI 933 GGATAC GTATCC(6/5) BlpI 250 GCTCAGC GC^T[ACGT]AGC Bpu1102I 250 GCTCAGC GC^T[ACGT]AGC BpuAI 799 GAAGAC GAAGAC(2/6) BsaAI 2048 TACGTA [CT]AC^GT[GA] BsaWI 3191 ACCGGA [AT]^CCGG[AT] BsmI 511 GCATTC GAATGC(1/-1) BspHI 3249 TCATGA T^CATGA BstBI 3119 TTCGAA TT^CGAA BstXI 2948 CCAATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 250 GCTCAGC GC^T[ACGT]AGC Eco47III 2680 AGCGCT AGC^GCT EcoT22I 601 ATGCAT ATGCA^T MaeII 2049 ACGT A^CGT MluNI 876 TGGCCA TGG^CCA MscI 876 TGGCCA TGG^CCA NsiI 601 ATGCAT ATGCA^T NspV 3119 TTCGAA TT^CGAA PflFI 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 958 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 3249 TCATGA T^CATGA SacI 2245 GAGCTC GAGCT^C SapI 2857 GAAGAGC GCTCTTC(1/4) SbfI 1938 CCTGCAGG CCTGCA^GG SexAI 963 ACCTGGT A^CC[AT]GGT SfuI 3119 TTCGAA TT^CGAA SmlI 609 CTCAAG C^T[CT][GA]AG SnaBI 2048 TACGTA TAC^GTA SpeI 190 ACTAGT A^CTAGT Sse8387I 1938 CCTGCAGG CCTGCA^GG SwaI 641 ATTTAAAT ATTT^AAAT TaqI 3120 TCGA T^CGA Tth111I 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 3120 TCGA T^CGA Van91I 958 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 342 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 2445 ACATGT A^C[GA][CT]GT 3029 ACATGT AseI 1476 ATTAAT AT^TAAT 2494 ATTAAT AsnI 1476 ATTAAT AT^TAAT 2494 ATTAAT AspHI 1076 GTGCAC G[AT]GC[AT]^C 2245 GAGCTC BanII 2189 GGGCTC G[GA]GC[CT]^C 2245 GAGCTC BseRI 1573 GAGGAG GAGGAG(10/8) 3100 GAGGAG BsgI 228 CTGCAC GTGCAG(16/14) 3157 GTGCAG BsiHKAI 1076 GTGCAC G[AT]GC[AT]^C 2245 GAGCTC Bsp1407I 1103 TGTACA T^GTACA 1955 TGTACA BspLU11I 2445 ACATGT A^CATGT 3029 ACATGT BsrGI 1103 TGTACA T^GTACA 1955 TGTACA BstAPI 241 GCAAGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2919 GCACAGGGTGC CfoI 2681 GCGC GCG^C 2862 GCGC DraIII 1259 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 2920 CACAGGGTG DsaI 221 CCATGG C^C[GA][CT]GG 2663 CCATGG EaeI 876 TGGCCA [CT]^GGCC[GA] 1559 TGGCCG HaeII 2680 AGCGCT [GA]GCGC^[CT] 2861 AGCGCC HhaI 2681 GCGC GCG^C 2862 GCGC HinP1I 2681 GCGC G^CGC 2862 GCGC HindIII 766 AAGCTT A^AGCTT 1732 AAGCTT NcoI 221 CCATGG C^CATGG 2663 CCATGG PleI 2212 GACTC GAGTC(4/5) 2572 GACTC PshBI 1476 ATTAAT AT^TAAT 2494 ATTAAT PstI 43 CTGCAG CTGCA^G 1939 CTGCAG SspBI 1103 TGTACA T^GTACA 1955 TGTACA SspI 1499 AATATT AAT^ATT 1978 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AvaII BbvI BcnI BfaI BglII BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI EarI Eco81I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII