(Circular) (Six-base) MAPSORT of: pFN21AE3947.seq Check: 6141 from: 1 to: 7999 pFN21AE3947 7999 bp With 182 enzymes: SgfI * March 28, 2012 11:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3911 3951 3911 Size: 40 7959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5809 278 Size: 53 83 186 936 4273 2468 Fragments arranged by size: 4273 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5211 5342 1937 Size: 3274 131 4594 Fragments arranged by size: 4594 3274 131 Acc65I G'GTAC_C Cuts at: 5320 5320 Size: 7999 AclI AA'CG_TT Cuts at: 1563 6126 6499 1563 Size: 4563 373 3063 Fragments arranged by size: 4563 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3408 4881 6062 7446 7714 1632 Size: 1776 1473 1181 1384 268 1917 Fragments arranged by size: 1917 1776 1473 1384 1181 268 AfeI AGC'GCT Cuts at: 4687 4687 Size: 7999 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7802 AflIII A'CryG_T Cuts at: 1203 4450 5034 6898 7875 1203 Size: 3247 584 1864 977 1327 Fragments arranged by size: 3247 1864 1327 977 584 AgeI A'CCGG_T Cuts at: 6830 6830 Size: 7999 AhdI GACnn_n'nnGTC Cuts at: 6728 6728 Size: 7999 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7999 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7999 AlwNI CAG_nnn'CTG Cuts at: 2243 3562 4213 5742 7314 2243 Size: 1319 651 1529 1572 2928 Fragments arranged by size: 2928 1572 1529 1319 651 ApaLI G'TGCA_C Cuts at: 3081 6056 7212 3081 Size: 2975 1156 3868 Fragments arranged by size: 3868 2975 1156 ApoI r'AATT_y Cuts at: 1493 2395 2820 3119 4260 4630 5308 5534 5686 Size: 902 425 299 1141 370 678 226 152 Cuts at: 5686 7795 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1141 902 678 425 370 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 3482 4500 6553 160 Size: 872 2450 1018 2053 1606 Fragments arranged by size: 2450 2053 1606 1018 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7999 AvaI C'yCGr_G Cuts at: 1870 1943 5324 1870 Size: 73 3381 4545 Fragments arranged by size: 4545 3381 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4799 4832 4799 Size: 33 7966 BamHI G'GATC_C Cuts at: 2118 5329 5696 2118 Size: 3211 367 4421 Fragments arranged by size: 4421 3211 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4015 5320 6775 618 Size: 325 217 627 2228 1305 1455 1842 Fragments arranged by size: 2228 1842 1455 1305 627 325 217 BanII G_rGCy'C Cuts at: 1391 4198 4254 5318 1391 Size: 2807 56 1064 4072 Fragments arranged by size: 4072 2807 1064 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2811 928 Size: 971 912 6116 Fragments arranged by size: 6116 971 912 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6190 6224 1903 Size: 34 50 34 4169 34 3678 Fragments arranged by size: 4169 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2932 5891 7101 2932 Size: 2959 1210 3830 Fragments arranged by size: 3830 2959 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7999 BfrBI ATG'CAT Cuts at: 2608 2608 Size: 7999 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6610 136 Size: 107 122 71 631 605 4938 1525 Fragments arranged by size: 4938 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2017 2201 3810 4327 7994 2017 Size: 184 1609 517 3667 2022 Fragments arranged by size: 3667 2022 1609 517 184 BlpI GC'TnA_GC Cuts at: 2256 5416 2256 Size: 3160 4839 Bme1580I G_kGCm'C Cuts at: 1790 3085 3601 6060 7216 1790 Size: 1295 516 2459 1156 2573 Fragments arranged by size: 2573 2459 1295 1156 516 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7999 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5730 6688 442 Size: 416 53 83 399 24 4313 958 1753 Fragments arranged by size: 4313 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7999 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2226 4644 6659 1239 Size: 891 96 2418 2015 2579 Fragments arranged by size: 2579 2418 2015 891 96 Bpu10I CC'TnA_GC Cuts at: 3474 3758 4253 3474 Size: 284 495 7220 Fragments arranged by size: 7220 495 284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2599 6124 6989 7287 2599 Size: 3525 865 298 3311 Fragments arranged by size: 3525 3311 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6662 882 Size: 617 5163 2219 Fragments arranged by size: 5163 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4055 5790 493 Size: 711 2851 1735 2702 Fragments arranged by size: 2851 2702 1735 711 BsaBI GATnn'nnATC Cuts at: 7993 7993 Size: 7999 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2299 2582 3585 3954 Size: 53 83 186 936 766 283 1003 369 Cuts at: 3954 5806 6188 6823 275 Size: 1852 382 635 1451 Fragments arranged by size: 1852 1451 1003 936 766 635 382 369 283 186 83 53 BsaWI w'CCGG_w Cuts at: 5196 6432 6830 7104 7251 7662 5196 Size: 1236 398 274 147 411 5533 Fragments arranged by size: 5533 1236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2007 2037 2067 2097 1178 Size: 30 165 30 604 30 30 30 7080 Fragments arranged by size: 7080 604 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3593 5120 1186 Size: 2407 1527 4065 Fragments arranged by size: 4065 2407 1527 BseYI C'CCAG_C Cuts at: 7202 7698 7202 Size: 496 7503 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 5183 1163 Size: 573 482 2965 3979 Fragments arranged by size: 3979 2965 573 482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5449 6210 7238 1540 Size: 275 186 3448 761 1028 2301 Fragments arranged by size: 3448 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3085 4254 6060 6145 7216 3085 Size: 1169 1806 85 1071 3868 Fragments arranged by size: 3868 1806 1169 1071 85 BsmI GAATG_Cn' Cuts at: 2516 5510 5603 2516 Size: 2994 93 4912 Fragments arranged by size: 4912 2994 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3085 3601 4198 4254 5318 6060 6145 Size: 399 1295 516 597 56 1064 742 85 Cuts at: 6145 7216 1391 Size: 1071 2174 Fragments arranged by size: 2174 1295 1071 1064 742 597 516 399 85 56 BspEI T'CCGG_A Cuts at: 7662 7662 Size: 7999 BspHI T'CATG_A Cuts at: 5254 5888 7618 5254 Size: 634 1730 5635 Fragments arranged by size: 5635 1730 634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 3054 3301 3911 3937 3951 5354 844 Size: 1381 829 247 610 26 14 1403 3489 Fragments arranged by size: 3489 1403 1381 829 610 247 26 14 BsrBI CCG'CTC Cuts at: 1394 1823 5886 1394 Size: 429 4063 3507 Fragments arranged by size: 4063 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3224 3324 4075 4141 6494 6668 65 Size: 1183 1976 100 751 66 2353 174 1396 Fragments arranged by size: 2353 1976 1396 1183 751 174 100 66 BsrFI r'CCGG_y Cuts at: 1700 1810 6643 6830 1700 Size: 110 4833 187 2869 Fragments arranged by size: 4833 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3108 3960 96 Size: 3012 852 4135 Fragments arranged by size: 4135 3012 852 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7999 BssSI C'ACGA_G Cuts at: 6059 7071 6059 Size: 1012 6987 BstAPI GCAn_nnn'nTGC Cuts at: 794 2252 4930 794 Size: 1458 2678 3863 Fragments arranged by size: 3863 2678 1458 BstBI TT'CG_AA Cuts at: 5125 6803 5125 Size: 1678 6321 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7999 BstXI CCAn_nnnn'nTGG Cuts at: 4960 4960 Size: 7999 BstYI r'GATC_y Cuts at: 1972 2017 2099 2118 2201 3810 4327 5329 5696 Size: 45 82 19 83 1609 517 1002 367 Cuts at: 5696 6081 6098 7550 7636 7648 7994 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1609 1452 1002 517 385 367 346 86 83 82 45 19 17 12 Bsu36I CC'TnA_GG Cuts at: 3126 3871 4302 3126 Size: 745 431 6823 Fragments arranged by size: 6823 745 431 BtgI C'CryG_G Cuts at: 513 1065 2226 4668 513 Size: 552 1161 2442 3844 Fragments arranged by size: 3844 2442 1161 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3777 4475 487 Size: 683 56 2551 698 4011 Fragments arranged by size: 4011 2551 698 683 56 BtsI GCAGTG_nn' Cuts at: 2184 2881 3825 4371 4884 5515 6309 6329 2184 Size: 697 944 546 513 631 794 20 3854 Fragments arranged by size: 3854 944 794 697 631 546 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7999 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3417 3452 529 Size: 35 2853 35 5076 Fragments arranged by size: 5076 2853 35 35 DraI TTT'AAA Cuts at: 2649 3028 4938 5303 2649 Size: 379 1910 365 5345 Fragments arranged by size: 5345 1910 379 365 DraIII CAC_nnn'GTG Cuts at: 3269 4930 3269 Size: 1661 6338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7006 809 Size: 507 5690 1802 Fragments arranged by size: 5690 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2881 3564 5446 Size: 54 1425 50 275 18 1051 683 1882 Cuts at: 5446 6335 7964 8 Size: 889 1629 43 Fragments arranged by size: 1882 1629 1425 1051 889 683 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5446 1537 Size: 275 3634 4090 Fragments arranged by size: 4090 3634 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3612 4142 4827 4857 5929 1353 Size: 2259 530 685 30 1072 3423 Fragments arranged by size: 3423 2259 1072 685 530 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6586 6960 7106 387 Size: 1064 138 4997 374 146 1280 Fragments arranged by size: 4997 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4252 4252 Size: 7999 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2226 3408 4644 4881 6062 6659 Size: 393 498 96 1182 1236 237 1181 597 Cuts at: 6659 7446 7714 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1236 1182 1181 787 597 498 393 268 237 96 EcoRI G'AATT_C Cuts at: 5308 5686 5308 Size: 378 7621 EcoRV GAT'ATC Cuts at: 2766 3280 3886 2766 Size: 514 606 6879 Fragments arranged by size: 6879 606 514 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2825 2909 5068 2825 Size: 84 2159 5756 Fragments arranged by size: 5756 2159 84 FspI TGC'GCA Cuts at: 6505 6505 Size: 7999 HaeII r_GCGC'y Cuts at: 1678 4689 4870 7146 1678 Size: 3011 181 2276 2531 Fragments arranged by size: 3011 2531 2276 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2103 2116 2135 4155 Size: 32 135 32 512 19 13 19 2020 Cuts at: 4155 4187 5249 5281 6640 6672 6714 6746 1373 Size: 32 1062 32 1359 32 42 32 2626 Fragments arranged by size: 2626 2020 1359 1062 512 135 42 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3646 3952 5005 5343 5589 669 Size: 988 281 1708 306 1053 338 246 3079 Fragments arranged by size: 3079 1708 1053 988 338 306 281 246 HindIII A'AGCT_T Cuts at: 748 2771 3737 748 Size: 2023 966 5010 Fragments arranged by size: 5010 2023 966 HpaI GTT'AAC Cuts at: 5589 5589 Size: 7999 KpnI G_GTAC'C Cuts at: 5324 5324 Size: 7999 MfeI C'AATT_G Cuts at: 5598 5598 Size: 7999 MluI A'CGCG_T Cuts at: 7875 7875 Size: 7999 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2434 3029 3407 4797 7113 7297 1305 Size: 1129 595 378 1390 2316 184 2007 Fragments arranged by size: 2316 2007 1390 1129 595 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2883 10 Size: 54 1425 343 1051 5126 Fragments arranged by size: 5126 1425 1051 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 2531 2751 4607 4746 4748 4877 Size: 648 895 470 220 1856 139 2 129 Cuts at: 4877 5957 6316 6475 518 Size: 1080 359 159 2042 Fragments arranged by size: 2042 1856 1080 895 648 470 359 220 159 139 129 2 MspA1I CmG'CkG Cuts at: 1606 5415 6092 7240 7485 1606 Size: 3809 677 1148 245 2120 Fragments arranged by size: 3809 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2226 4668 513 Size: 552 1161 2442 3844 Fragments arranged by size: 3844 2442 1161 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7999 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7999 NotI GC'GGCC_GC Cuts at: 5446 5446 Size: 7999 NsiI A_TGCA'T Cuts at: 2610 2610 Size: 7999 NspI r_CATG'y Cuts at: 1132 1229 2546 4351 4454 4606 5038 5357 6902 Size: 97 1317 1805 103 152 432 319 1545 Cuts at: 6902 1132 Size: 2229 Fragments arranged by size: 2229 1805 1545 1317 432 319 152 103 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7999 PciI A'CATG_T Cuts at: 4450 5034 6898 4450 Size: 584 1864 5551 Fragments arranged by size: 5551 1864 584 PflMI CCAn_nnn'nTGG Cuts at: 1108 2969 1108 Size: 1861 6138 PfoI T'CCnGG_A Cuts at: 4212 4212 Size: 7999 PmeI GTTT'AAAC Cuts at: 5303 5303 Size: 7999 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6068 6100 7581 7613 6068 Size: 32 1481 32 6454 Fragments arranged by size: 6454 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7999 PsiI TTA'TAA Cuts at: 2700 5569 2700 Size: 2869 5130 PstI C_TGCA'G Cuts at: 830 2052 3948 5351 830 Size: 1222 1896 1403 3478 Fragments arranged by size: 3478 1896 1403 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7999 SacI G_AGCT'C Cuts at: 4254 4254 Size: 7999 SalI G'TCGA_C Cuts at: 1936 5341 1936 Size: 3405 4594 SapI GCTCTTCn'nnn_ Cuts at: 4857 4857 Size: 7999 SbfI CC_TGCA'GG Cuts at: 3948 5351 3948 Size: 1403 6596 ScaI AGT'ACT Cuts at: 6247 7959 6247 Size: 1712 6287 SexAI A'CCwGG_T Cuts at: 2968 2968 Size: 7999 SfcI C'TryA_G Cuts at: 826 1045 2048 3944 4405 5347 6482 7163 7354 Size: 219 1003 1896 461 942 1135 681 191 Cuts at: 7354 826 Size: 1471 Fragments arranged by size: 1896 1471 1135 1003 942 681 461 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7999 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7999 SmaI CCC'GGG Cuts at: 1872 5326 1872 Size: 3454 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2614 6103 7004 7266 820 Size: 197 926 671 3489 901 262 1553 Fragments arranged by size: 3489 1553 926 901 671 262 197 SnaBI TAC'GTA Cuts at: 493 4055 5790 493 Size: 3562 1735 2702 Fragments arranged by size: 3562 2702 1735 SpeI A'CTAG_T Cuts at: 152 2195 152 Size: 2043 5956 SphI G_CATG'C Cuts at: 1132 5357 1132 Size: 4225 3774 SspI AAT'ATT Cuts at: 5 52 3506 3985 5923 5 Size: 47 3454 479 1938 2081 Fragments arranged by size: 3454 2081 1938 479 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7999 StyI C'CwwG_G Cuts at: 513 1065 2086 2226 4668 4796 5189 5438 513 Size: 552 1021 140 2442 128 393 249 3074 Fragments arranged by size: 3074 2442 1021 552 393 249 140 128 SwaI ATTT'AAAT Cuts at: 2649 2649 Size: 7999 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4788 6026 6043 6196 6381 6826 325 Size: 1255 3208 1238 17 153 185 445 1498 Fragments arranged by size: 3208 1498 1255 1238 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3108 3960 Size: 274 80 33 51 1443 117 1014 852 Cuts at: 3960 5017 6245 7957 96 Size: 1057 1228 1712 138 Fragments arranged by size: 1712 1443 1228 1057 1014 852 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4701 4733 4904 4936 7959 7991 4701 Size: 32 171 32 3023 32 4709 Fragments arranged by size: 4709 3023 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2551 1316 Size: 1235 6764 XbaI T'CTAG_A Cuts at: 2347 5335 2347 Size: 2988 5011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7999 XmaI C'CCGG_G Cuts at: 1870 5324 1870 Size: 3454 4545 XmnI GAAnn'nnTTC Cuts at: 6128 6128 Size: 7999 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5807 276 Size: 53 83 186 936 4273 2468 Fragments arranged by size: 4273 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI XcmI