Restriction map for
pFN21AE3947
(Linear) (Six-base) MAPSORT of: KIEE3947.seq Check: 1791 from: 1 to: 3294
KIEE3947.orf
With 182 enzymes: SgfI *
March 22, 2012 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1906 1946 3294
Size: 1906 40 1348
Fragments arranged by size:
1906 1348 40
AccI GT'mk_AC
Cuts at: 0 3206 3294
Size: 3206 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 2876 3294
Size: 1403 1473 418
Fragments arranged by size:
1473 1403 418
AfeI AGC'GCT
Cuts at: 0 2682 3294
Size: 2682 612
AflIII A'CryG_T
Cuts at: 0 2445 3029 3294
Size: 2445 584 265
Fragments arranged by size:
2445 584 265
AlwNI CAG_nnn'CTG
Cuts at: 0 238 1557 2208 3294
Size: 238 1319 651 1086
Fragments arranged by size:
1319 1086 651 238
ApaLI G'TGCA_C
Cuts at: 0 1076 3294
Size: 1076 2218
ApoI r'AATT_y
Cuts at: 0 390 815 1114 2255 2625 3294
Size: 390 425 299 1141 370 669
Fragments arranged by size:
1141 669 425 390 370 299
AseI AT'TA_AT
Cuts at: 0 1477 2495 3294
Size: 1477 1018 799
Fragments arranged by size:
1477 1018 799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2794 2827 3294
Size: 2794 33 467
Fragments arranged by size:
2794 467 33
BamHI G'GATC_C
Cuts at: 0 113 3294
Size: 113 3181
BanI G'GyrC_C
Cuts at: 0 2010 3294
Size: 2010 1284
BanII G_rGCy'C
Cuts at: 0 2193 2249 3294
Size: 2193 56 1045
Fragments arranged by size:
2193 1045 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 3294
Size: 806 2488
BciVI GTATCCnnnnn_n'
Cuts at: 0 927 3294
Size: 927 2367
BfrBI ATG'CAT
Cuts at: 0 603 3294
Size: 603 2691
BglII A'GATC_T
Cuts at: 0 12 196 1805 2322 3294
Size: 12 184 1609 517 972
Fragments arranged by size:
1609 972 517 184 12
BlpI GC'TnA_GC
Cuts at: 0 251 3294
Size: 251 3043
Bme1580I G_kGCm'C
Cuts at: 0 1080 1596 3294
Size: 1080 516 1698
Fragments arranged by size:
1698 1080 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 221 2639 3294
Size: 125 96 2418 655
Fragments arranged by size:
2418 655 125 96
Bpu10I CC'TnA_GC
Cuts at: 0 1469 1753 2248 3294
Size: 1469 284 495 1046
Fragments arranged by size:
1469 1046 495 284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 3294
Size: 594 2700
BsaAI yAC'GTr
Cuts at: 0 2050 3294
Size: 2050 1244
BsaHI Gr'CG_yC
Cuts at: 0 294 577 1580 1949 3294
Size: 294 283 1003 369 1345
Fragments arranged by size:
1345 1003 369 294 283
BsaWI w'CCGG_w
Cuts at: 0 3191 3294
Size: 3191 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 62 92 3294
Size: 2 30 30 30 3202
Fragments arranged by size:
3202 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1588 3115 3294
Size: 1588 1527 179
Fragments arranged by size:
1588 1527 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 3178 3294
Size: 213 2965 116
Fragments arranged by size:
2965 213 116
BsiHKAI G_wGCw'C
Cuts at: 0 1080 2249 3294
Size: 1080 1169 1045
Fragments arranged by size:
1169 1080 1045
BsmI GAATG_Cn'
Cuts at: 0 511 3294
Size: 511 2783
Bsp1286I G_dGCh'C
Cuts at: 0 1080 1596 2193 2249 3294
Size: 1080 516 597 56 1045
Fragments arranged by size:
1080 1045 597 516 56
BspHI T'CATG_A
Cuts at: 0 3249 3294
Size: 3249 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 1049 1296 1906 1932 1946 3294
Size: 220 829 247 610 26 14 1348
Fragments arranged by size:
1348 829 610 247 220 26 14
BsrDI GCAATG_nn'
Cuts at: 0 1219 1319 2070 2136 3294
Size: 1219 100 751 66 1158
Fragments arranged by size:
1219 1158 751 100 66
BsrGI T'GTAC_A
Cuts at: 0 1103 1955 3294
Size: 1103 852 1339
Fragments arranged by size:
1339 1103 852
BstAPI GCAn_nnn'nTGC
Cuts at: 0 247 2925 3294
Size: 247 2678 369
Fragments arranged by size:
2678 369 247
BstBI TT'CG_AA
Cuts at: 0 3120 3294
Size: 3120 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2955 3294
Size: 2955 339
BstYI r'GATC_y
Cuts at: 0 12 94 113 196 1805 2322 3294
Size: 12 82 19 83 1609 517 972
Fragments arranged by size:
1609 972 517 83 82 19 12
Bsu36I CC'TnA_GG
Cuts at: 0 1121 1866 2297 3294
Size: 1121 745 431 997
Fragments arranged by size:
1121 997 745 431
BtgI C'CryG_G
Cuts at: 0 221 2663 3294
Size: 221 2442 631
Fragments arranged by size:
2442 631 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1772 2470 3294
Size: 1772 698 824
Fragments arranged by size:
1772 824 698
BtsI GCAGTG_nn'
Cuts at: 0 179 876 1820 2366 2879 3294
Size: 179 697 944 546 513 415
Fragments arranged by size:
944 697 546 513 415 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1412 1447 3294
Size: 1412 35 1847
Fragments arranged by size:
1847 1412 35
DraI TTT'AAA
Cuts at: 0 644 1023 2933 3294
Size: 644 379 1910 361
Fragments arranged by size:
1910 644 379 361
DraIII CAC_nnn'GTG
Cuts at: 0 1264 2925 3294
Size: 1264 1661 369
Fragments arranged by size:
1661 1264 369
EaeI y'GGCC_r
Cuts at: 0 876 1559 3294
Size: 876 683 1735
Fragments arranged by size:
1735 876 683
EarI CTCTTCn'nnn_
Cuts at: 0 1607 2137 2822 2852 3294
Size: 1607 530 685 30 442
Fragments arranged by size:
1607 685 530 442 30
EcoICRI GAG'CTC
Cuts at: 0 2247 3294
Size: 2247 1047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 221 1403 2639 2876 3294
Size: 125 96 1182 1236 237 418
Fragments arranged by size:
1236 1182 418 237 125 96
EcoRV GAT'ATC
Cuts at: 0 761 1275 1881 3294
Size: 761 514 606 1413
Fragments arranged by size:
1413 761 606 514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 820 904 3063 3294
Size: 820 84 2159 231
Fragments arranged by size:
2159 820 231 84
HaeII r_GCGC'y
Cuts at: 0 2684 2865 3294
Size: 2684 181 429
Fragments arranged by size:
2684 429 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 98 111 130 2150 2182 3244 3276
Size: 79 19 13 19 2020 32 1062 32
Cuts at: 3276 3294
Size: 18
Fragments arranged by size:
2020 1062 79 32 32 19 19 18
13
HincII GTy'rAC
Cuts at: 0 1641 1947 3000 3294
Size: 1641 306 1053 294
Fragments arranged by size:
1641 1053 306 294
HindIII A'AGCT_T
Cuts at: 0 766 1732 3294
Size: 766 966 1562
Fragments arranged by size:
1562 966 766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 1024 1402 2792 3294
Size: 429 595 378 1390 502
Fragments arranged by size:
1390 595 502 429 378
MscI TGG'CCA
Cuts at: 0 878 3294
Size: 878 2416
MslI CAynn'nnrTG
Cuts at: 0 56 526 746 2602 2741 2743 2872 3294
Size: 56 470 220 1856 139 2 129 422
Fragments arranged by size:
1856 470 422 220 139 129 56 2
NcoI C'CATG_G
Cuts at: 0 221 2663 3294
Size: 221 2442 631
Fragments arranged by size:
2442 631 221
NsiI A_TGCA'T
Cuts at: 0 605 3294
Size: 605 2689
NspI r_CATG'y
Cuts at: 0 541 2346 2449 2601 3033 3294
Size: 541 1805 103 152 432 261
Fragments arranged by size:
1805 541 432 261 152 103
PciI A'CATG_T
Cuts at: 0 2445 3029 3294
Size: 2445 584 265
Fragments arranged by size:
2445 584 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 964 3294
Size: 964 2330
PfoI T'CCnGG_A
Cuts at: 0 2207 3294
Size: 2207 1087
PsiI TTA'TAA
Cuts at: 0 695 3294
Size: 695 2599
PstI C_TGCA'G
Cuts at: 0 47 1943 3294
Size: 47 1896 1351
Fragments arranged by size:
1896 1351 47
SacI G_AGCT'C
Cuts at: 0 2249 3294
Size: 2249 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 2852 3294
Size: 2852 442
SbfI CC_TGCA'GG
Cuts at: 0 1943 3294
Size: 1943 1351
SexAI A'CCwGG_T
Cuts at: 0 963 3294
Size: 963 2331
SfcI C'TryA_G
Cuts at: 0 43 1939 2400 3294
Size: 43 1896 461 894
Fragments arranged by size:
1896 894 461 43
SmlI C'TyrA_G
Cuts at: 0 609 3294
Size: 609 2685
SnaBI TAC'GTA
Cuts at: 0 2050 3294
Size: 2050 1244
SpeI A'CTAG_T
Cuts at: 0 190 3294
Size: 190 3104
SspI AAT'ATT
Cuts at: 0 1501 1980 3294
Size: 1501 479 1314
Fragments arranged by size:
1501 1314 479
StyI C'CwwG_G
Cuts at: 0 81 221 2663 2791 3184 3294
Size: 81 140 2442 128 393 110
Fragments arranged by size:
2442 393 140 128 110 81
SwaI ATTT'AAAT
Cuts at: 0 644 3294
Size: 644 2650
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2783 3294
Size: 2783 511
TatI w'GTAC_w
Cuts at: 0 89 1103 1955 3012 3294
Size: 89 1014 852 1057 282
Fragments arranged by size:
1057 1014 852 282 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2696 2728 2899 2931 3294
Size: 2696 32 171 32 363
Fragments arranged by size:
2696 363 171 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 546 3294
Size: 546 2748
XbaI T'CTAG_A
Cuts at: 0 342 3294
Size: 342 2952
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaLI ApoI
AseI BaeI BamHI BanI BanII BbsI BciVI BfrBI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI NcoI NsiI NspI PciI PflMI
PfoI PsiI PstI SacI SapI SbfI SexAI SfcI
SmlI SnaBI SpeI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BbeI BbvCI
BcgI BclI BglI BmgBI BmrI BmtI BplI BsaI
BsaBI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstEII BstZ17I ClaI DrdI EagI EciI
EcoNI EcoO109I EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI
SmaI SphI SrfI StuI XcmI XhoI XmaI XmnI
ZraI