################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AspHI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tsp45I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 626 GGTACC G^GTACC AccI 742 GTAGAC GT^[AC][GT]AC AciI 350 CCGC CCGC(-3/-1) AcyI 832 GGCGCC G[GA]^CG[CT]C AgeI 178 ACCGGT A^CCGGT AlwI 430 GATCC GGATC(4/5) Aor51HI 302 AGCGCT AGC^GCT Asp700I 514 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 626 GGTACC G^GTACC AspI 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 167 CTCGGG C^[CT]CG[GA]G AvaII 240 GGACC G^G[AT]CC BbeI 832 GGCGCC GGCGC^C BcnI 74 CCGGG CC^[GC]GG BglII 807 AGATCT A^GATCT BpmI 566 CTGGAG CTGGAG(16/14) BsaBI 901 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 832 GGCGCC G[GA]^CG[CT]C BsaWI 178 ACCGGT [AT]^CCGG[AT] BsmAI 606 GTCTC GTCTC(1/5) BsmI 981 GAATGC GAATGC(1/-1) BsoBI 167 CTCGGG C^[CT]CG[GA]G BsrFI 178 ACCGGT [GA]^CCGG[CT] BstYI 807 AGATCT [GA]^GATC[CT] Cfr10I 178 ACCGGT [GA]^CCGG[CT] DrdI 1153 GACCTGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 302 AGCGCT AGC^GCT EcoNI 1011 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 832 GGCGCC G[GA]^CG[CT]C KasI 832 GGCGCC G^GCGCC KpnI 626 GGTACC GGTAC^C MaeII 749 ACGT A^CGT MamI 901 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 807 AGATCT [GA]^GATC[CT] NarI 832 GGCGCC GG^CGCC NciI 74 CCGGG CC^[GC]GG NcoI 828 CCATGG C^CATGG NspI 851 ACATGC [GA]CATG^[CT] PflFI 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 178 ACCGGT A^CCGGT PleI 84 GACTC GAGTC(4/5) PpuMI 239 GGGACCC [GA]G^G[AT]CC[CT] PstI 974 CTGCAG CTGCA^G PvuII 757 CAGCTG CAG^CTG SapI 481 GCTCTTC GCTCTTC(1/4) SfoI 832 GGCGCC GGC^GCC SspI 635 AATATT AAT^ATT TaqI 804 TCGA T^CGA Tth111I 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 804 TCGA T^CGA XhoII 807 AGATCT [GA]^GATC[CT] XmnI 514 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 668 GAATTT [GA]^AATT[CT] 763 AAATTC AfaI 627 GTAC GT^AC 1029 GTAC ApoI 668 GAATTT [GA]^AATT[CT] 763 AAATTC BsmFI 239 GGGAC GGGAC(10/14) 1162 GTCCC BspMI 1154 ACCTGC ACCTGC(4/8) 196 GCAGGT BsrI 187 ACTGG ACTGG(1/-1) 737 CCAGT BstAPI 196 GCAGGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 976 GCAGGGAATGC CfoI 303 GCGC GCG^C 833 GCGC DdeI 89 CTGAG C^T[ACGT]AG 1065 CTTAG DsaI 828 CCATGG C^C[GA][CT]GG 966 CCACGG EaeI 50 TGGCCG [CT]^GGCC[GA] 347 TGGCCG EarI 482 CTCTTC CTCTTC(1/4) 585 GAAGAG EcoT14I 708 CCAAGG C^C[AT][AT]GG 828 CCATGG HaeII 302 AGCGCT [GA]GCGC^[CT] 832 GGCGCC HapII 74 CCGG C^CGG 179 CCGG HhaI 303 GCGC GCG^C 833 GCGC HinP1I 303 GCGC G^CGC 833 GCGC HpaII 74 CCGG C^CGG 179 CCGG Ksp632I 482 CTCTTC CTCTTC(1/4) 585 GAAGAG MaeIII 182 GTTAC ^GT[ACGT]AC 717 GTTAC MseI 295 TTAA T^TAA 559 TTAA MslI 220 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 265 CATGGCTATG MspA1I 350 CCGCTG C[AC]G^C[GT]G 757 CAGCTG MspI 74 CCGG C^CGG 179 CCGG RsaI 627 GTAC GT^AC 1029 GTAC SfcI 253 CTACAG C^T[GA][CT]AG 974 CTGCAG StuI 147 AGGCCT AGG^CCT 863 AGGCCT StyI 708 CCAAGG C^C[AT][AT]GG 828 CCATGG Tru9I 295 TTAA T^TAA 559 TTAA XcmI 819 CCATTGGTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 828 CCATGGCGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AluI BanI BbvI BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI