(Circular) (Six-base) MAPSORT of: pFN21AE3674.seq Check: 6413 from: 1 to: 5902 pFN21AE3674 5902 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2748 3245 1937 Size: 811 497 4594 Fragments arranged by size: 4594 811 497 Acc65I G'GTAC_C Cuts at: 2631 3223 2631 Size: 592 5310 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2969 3965 5349 5617 1632 Size: 488 849 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 849 488 268 AfeI AGC'GCT Cuts at: 2309 2309 Size: 5902 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 2183 4733 2183 Size: 2550 3352 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2416 1184 Size: 1232 4670 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2673 2768 3211 3437 3589 5698 1493 Size: 1180 95 443 226 152 2109 1697 Fragments arranged by size: 2109 1697 1180 443 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3227 1870 Size: 73 229 1055 4545 Fragments arranged by size: 4545 1055 229 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2631 2829 2837 3223 Size: 325 217 627 393 451 198 8 386 Cuts at: 3223 4678 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 451 393 386 325 217 198 8 BanII G_rGCy'C Cuts at: 1391 3221 1391 Size: 1830 4072 BbeI G_GCGC'C Cuts at: 2841 2841 Size: 5902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2812 5897 2812 Size: 3085 2817 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2736 3633 4591 Size: 416 53 83 399 24 1319 897 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1319 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2106 2106 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 4562 1239 Size: 1353 1970 2579 Fragments arranged by size: 2579 1970 1353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 2910 5896 2910 Size: 2986 2916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2838 3709 4091 4726 Size: 53 83 186 936 1305 871 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1451 1305 936 871 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2183 4335 4733 5007 5154 5565 2183 Size: 2152 398 274 147 411 2520 Fragments arranged by size: 2520 2152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5902 BseYI C'CCAG_C Cuts at: 2196 2696 5105 5601 2196 Size: 500 2409 496 2497 Fragments arranged by size: 2497 2409 500 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 2992 3413 3506 2992 Size: 421 93 5388 Fragments arranged by size: 5388 421 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3221 3963 4048 5119 1391 Size: 399 1431 742 85 1071 2174 Fragments arranged by size: 2174 1431 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 3168 3257 844 Size: 1348 976 89 3489 Fragments arranged by size: 3489 1348 976 89 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2183 4546 4733 1700 Size: 110 373 2363 187 2869 Fragments arranged by size: 2869 2363 373 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 2207 2987 794 Size: 1413 780 3709 Fragments arranged by size: 3709 1413 780 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2812 3232 3599 3984 4001 5453 5539 5551 Size: 840 420 367 385 17 1452 86 12 Cuts at: 5551 5897 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 840 420 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2833 2971 513 Size: 552 939 829 138 3444 Fragments arranged by size: 3444 939 829 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2355 3418 4212 4232 2355 Size: 1063 794 20 4025 Fragments arranged by size: 4025 1063 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3164 4909 809 Size: 507 1848 1745 1802 Fragments arranged by size: 1848 1802 1745 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2352 3349 Size: 54 1425 50 275 18 225 297 997 Cuts at: 3349 4238 5867 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 997 889 297 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 2585 3832 1353 Size: 1140 92 1247 3423 Fragments arranged by size: 3423 1247 1140 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2592 2969 3965 4562 5349 5617 Size: 393 488 472 377 996 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 996 787 597 488 472 393 377 268 EcoNI CCTnn'n_nnAGG Cuts at: 3020 3020 Size: 5902 EcoO109I rG'GnC_Cy Cuts at: 2245 2339 3045 2245 Size: 94 706 5102 Fragments arranged by size: 5102 706 94 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2311 2841 5049 1678 Size: 633 530 2208 2531 Fragments arranged by size: 2531 2208 633 530 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 4543 4575 4617 Size: 32 135 32 850 32 2089 32 42 Cuts at: 4617 4649 1373 Size: 32 2626 Fragments arranged by size: 2626 2089 850 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KasI G'GCGC_C Cuts at: 2837 2837 Size: 5902 KpnI G_GTAC'C Cuts at: 2635 3227 2635 Size: 592 5310 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 2274 3860 4219 4378 518 Size: 648 1063 45 1586 359 159 2042 Fragments arranged by size: 2042 1586 1063 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2357 2764 3318 3995 5143 5388 1606 Size: 751 407 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 751 677 554 407 245 NarI GG'CG_CC Cuts at: 2838 2838 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 2004 2833 513 Size: 552 939 829 3582 Fragments arranged by size: 3582 939 829 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2860 3260 4805 1132 Size: 97 1631 400 1545 2229 Fragments arranged by size: 2229 1631 1545 400 97 PasI CC'CwG_GG Cuts at: 1348 2843 1348 Size: 1495 4407 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2245 Size: 5902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 2043 3472 2043 Size: 1429 4473 PstI C_TGCA'G Cuts at: 830 2983 3254 830 Size: 2153 271 3478 Fragments arranged by size: 3478 2153 271 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SanDI GG'GwC_CC Cuts at: 2245 2245 Size: 5902 SapI GCTCTTCn'nnn_ Cuts at: 2493 2493 Size: 5902 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2258 2979 3250 4385 5066 5257 826 Size: 219 1213 721 271 1135 681 191 1471 Fragments arranged by size: 1471 1213 1135 721 681 271 219 191 SfoI GGC'GCC Cuts at: 2839 2839 Size: 5902 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2642 3826 5 Size: 47 2590 1184 2081 Fragments arranged by size: 2590 2081 1184 47 StuI AGG'CCT Cuts at: 1517 2154 2870 1517 Size: 637 716 4549 Fragments arranged by size: 4549 716 637 StyI C'CwwG_G Cuts at: 513 1065 2004 2713 2833 3341 513 Size: 552 939 709 120 508 3074 Fragments arranged by size: 3074 939 709 552 508 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3929 3946 4099 4284 4729 325 Size: 1255 2349 17 153 185 445 1498 Fragments arranged by size: 2349 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 2753 1316 Size: 1437 4465 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2831 2840 2831 Size: 9 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 2523 4031 2523 Size: 1508 4394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI