################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AflII AflIII AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AvrII BciVI BclI BfrI BlnI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI Eam1105I EcoRI EcoRV FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PmeI PshBI PvuI RcaI RsrII SacI SacII SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 2605 GTCGAC GT^[AC][GT]AC AclI 408 AACGTT AA^CGTT AgeI 698 ACCGGT A^CCGGT Aor51HI 2674 AGCGCT AGC^GCT AvaI 2333 CCCGGG C^[CT]CG[GA]G AviII 2780 TGCGCA TGC^GCA BamHI 731 GGATCC G^GATCC BbrPI 769 CACGTG CAC^GTG BbvCI 2061 GCTGAGG CCTCAGC(-5/-2) BcgI 15 GCACAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 769 CACGTG [CT]AC^GT[GA] BsiEI 77 CGGCCG CG[GA][CT]^CG BsmI 232 GCATTC GAATGC(1/-1) BsoBI 2333 CCCGGG C^[CT]CG[GA]G BsrFI 698 ACCGGT [GA]^CCGG[CT] BssHII 1964 GCGCGC G^CGCGC BstAPI 1467 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 698 ACCGGT [GA]^CCGG[CT] DraI 958 TTTAAA TTT^AAA EagI 77 CGGCCG C^GGCCG EclXI 77 CGGCCG C^GGCCG Eco47III 2674 AGCGCT AGC^GCT Eco52I 77 CGGCCG C^GGCCG EcoT22I 2837 ATGCAT ATGCA^T FspI 2780 TGCGCA TGC^GCA HincII 2605 GTCGAC GT[CT]^[GA]AC HindII 2605 GTCGAC GT[CT]^[GA]AC NruI 2277 TCGCGA TCG^CGA NsiI 2837 ATGCAT ATGCA^T PflMI 2763 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 698 ACCGGT A^CCGGT PmaCI 769 CACGTG CAC^GTG PmlI 769 CACGTG CAC^GTG PpuMI 2297 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 336 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 408 AACGTT AA^CGTT SalI 2605 GTCGAC G^TCGAC SbfI 1536 CCTGCAGG CCTGCA^GG SfiI 1926 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2333 CCCGGG CCC^GGG Sse8387I 1536 CCTGCAGG CCTGCA^GG TfiI 620 GAATC G^A[AT]TC Van91I 2763 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2333 CCCGGG C^CCGGG XmaI 2333 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 1965 CGCG CG^CG 2278 CGCG AocI 226 CCTCAGG CC^T[ACGT]AGG 2509 CCTGAGG Asp700I 994 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2859 GAAGGCTTTC BalI 1385 TGGCCA TGG^CCA 1907 TGGCCA BbeI 872 GGCGCC GGCGC^C 1083 GGCGCC BlpI 146 GCTTAGC GC^T[ACGT]AGC 2139 GCTGAGC Bpu1102I 146 GCTTAGC GC^T[ACGT]AGC 2139 GCTGAGC BsaWI 698 ACCGGT [AT]^CCGG[AT] 2654 TCCGGT BsrBI 1273 GAGCGG CCGCTC(-3/-3) 1639 GAGCGG BssSI 1552 CACGAG CACGAG(-5/-1) 2731 CACGAG BstEII 718 GGTAACC G^GT[ACGT]ACC 1705 GGTAACC BstPI 718 GGTAACC G^GT[ACGT]ACC 1705 GGTAACC BstUI 1965 CGCG CG^CG 2278 CGCG BstXI 65 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1852 CCAGGTGAATGG Bsu36I 226 CCTCAGG CC^T[ACGT]AGG 2509 CCTGAGG CelII 146 GCTTAGC GC^T[ACGT]AGC 2139 GCTGAGC Eco81I 226 CCTCAGG CC^T[ACGT]AGG 2509 CCTGAGG EcoNI 795 CCTACTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2884 CCTCCCGAAGG EcoO65I 718 GGTAACC G^GT[ACGT]ACC 1705 GGTAACC HgaI 2025 GACGC GACGC(5/10) 2323 GACGC KasI 872 GGCGCC G^GCGCC 1083 GGCGCC MluNI 1385 TGGCCA TGG^CCA 1907 TGGCCA MscI 1385 TGGCCA TGG^CCA 1907 TGGCCA MvnI 1965 CGCG CG^CG 2278 CGCG NarI 872 GGCGCC GG^CGCC 1083 GGCGCC PleI 316 GACTC GAGTC(4/5) 1843 GAGTC SfoI 872 GGCGCC GGC^GCC 1083 GGCGCC SmlI 1315 CTTGAG C^T[CT][GA]AG 2199 CTTGAG StuI 1439 AGGCCT AGG^CCT 2881 AGGCCT XmnI 994 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2859 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII