(Linear) (Six-base) MAPSORT of: KIBB8537.seq Check: 6226 from: 1 to: 2916 KIBB8537.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1458 1530 2916 Size: 1458 72 1386 Fragments arranged by size: 1458 1386 72 AccI GT'mk_AC Cuts at: 0 2606 2916 Size: 2606 310 AclI AA'CG_TT Cuts at: 0 409 2916 Size: 409 2507 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1859 2878 2916 Size: 1859 1019 38 Fragments arranged by size: 1859 1019 38 AfeI AGC'GCT Cuts at: 0 2676 2916 Size: 2676 240 AgeI A'CCGG_T Cuts at: 0 698 2916 Size: 698 2218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 178 1113 1150 1704 2374 2916 Size: 178 935 37 554 670 542 Fragments arranged by size: 935 670 554 542 178 37 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 264 296 2916 Size: 264 32 2620 Fragments arranged by size: 2620 264 32 AlwNI CAG_nnn'CTG Cuts at: 0 2028 2409 2718 2769 2916 Size: 2028 381 309 51 147 Fragments arranged by size: 2028 381 309 147 51 ApaI G_GGCC'C Cuts at: 0 42 953 2314 2916 Size: 42 911 1361 602 Fragments arranged by size: 1361 911 602 42 ApoI r'AATT_y Cuts at: 0 139 994 1141 2475 2916 Size: 139 855 147 1334 441 Fragments arranged by size: 1334 855 441 147 139 AvaI C'yCGr_G Cuts at: 0 2333 2916 Size: 2333 583 BamHI G'GATC_C Cuts at: 0 731 2916 Size: 731 2185 BanI G'GyrC_C Cuts at: 0 31 679 872 1083 2916 Size: 31 648 193 211 1833 Fragments arranged by size: 1833 648 211 193 31 BanII G_rGCy'C Cuts at: 0 42 131 403 953 2314 2916 Size: 42 89 272 550 1361 602 Fragments arranged by size: 1361 602 550 272 89 42 BbeI G_GCGC'C Cuts at: 0 876 1087 2916 Size: 876 211 1829 Fragments arranged by size: 1829 876 211 BbsI GAAGACnn'nnnn_ Cuts at: 0 1662 1748 2007 2748 2916 Size: 1662 86 259 741 168 Fragments arranged by size: 1662 741 259 168 86 BbvCI CC'TCA_GC Cuts at: 0 2062 2916 Size: 2062 854 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4 38 2916 Size: 4 34 2878 Fragments arranged by size: 2878 34 4 BfrBI ATG'CAT Cuts at: 0 2839 2916 Size: 2839 77 BglI GCCn_nnn'nGGC Cuts at: 0 1336 1438 1933 2916 Size: 1336 102 495 983 Fragments arranged by size: 1336 983 495 102 BglII A'GATC_T Cuts at: 0 373 423 2630 2916 Size: 373 50 2207 286 Fragments arranged by size: 2207 373 286 50 BlpI GC'TnA_GC Cuts at: 0 147 2140 2916 Size: 147 1993 776 Fragments arranged by size: 1993 776 147 Bme1580I G_kGCm'C Cuts at: 0 42 776 953 2314 2916 Size: 42 734 177 1361 602 Fragments arranged by size: 1361 734 602 177 42 BmgBI CAC'GTC Cuts at: 0 72 2010 2543 2916 Size: 72 1938 533 373 Fragments arranged by size: 1938 533 373 72 BmrI ACTGGGnnnn_n' Cuts at: 0 406 882 2118 2916 Size: 406 476 1236 798 Fragments arranged by size: 1236 798 476 406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 757 850 2191 2728 2791 2916 Size: 415 342 93 1341 537 63 125 Fragments arranged by size: 1341 537 415 342 125 93 63 Bpu10I CC'TnA_GC Cuts at: 0 1987 2062 2678 2916 Size: 1987 75 616 238 Fragments arranged by size: 1987 616 238 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1336 2220 2916 Size: 1336 884 696 Fragments arranged by size: 1336 884 696 BsaI GGTCTCn'nnnn_ Cuts at: 0 1126 1144 2534 2916 Size: 1126 18 1390 382 Fragments arranged by size: 1390 1126 382 18 BsaAI yAC'GTr Cuts at: 0 771 2916 Size: 771 2145 BsaHI Gr'CG_yC Cuts at: 0 873 1084 2324 2916 Size: 873 211 1240 592 Fragments arranged by size: 1240 873 592 211 BsaWI w'CCGG_w Cuts at: 0 698 2654 2916 Size: 698 1956 262 Fragments arranged by size: 1956 698 262 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 207 237 511 541 1417 1447 1558 1588 Size: 207 30 274 30 876 30 111 30 Cuts at: 1588 2916 Size: 1328 Fragments arranged by size: 1328 876 274 207 111 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 215 535 847 1474 1477 1570 1573 1755 Size: 215 320 312 627 3 93 3 182 Cuts at: 1755 2170 2916 Size: 415 746 Fragments arranged by size: 746 627 415 320 312 215 182 93 3 3 BseYI C'CCAG_C Cuts at: 0 87 837 857 2916 Size: 87 750 20 2059 Fragments arranged by size: 2059 750 87 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 1120 1397 1674 1700 2386 2916 Size: 751 369 277 277 26 686 530 Fragments arranged by size: 751 686 530 369 277 277 26 BsiEI CG_ry'CG Cuts at: 0 80 2916 Size: 80 2836 BsiHKAI G_wGCw'C Cuts at: 0 17 761 2146 2916 Size: 17 744 1385 770 Fragments arranged by size: 1385 770 744 17 BsmI GAATG_Cn' Cuts at: 0 232 2916 Size: 232 2684 Bsp1286I G_dGCh'C Cuts at: 0 17 42 131 403 761 776 953 2146 Size: 17 25 89 272 358 15 177 1193 Cuts at: 2146 2314 2916 Size: 168 602 Fragments arranged by size: 1193 602 358 272 177 168 89 25 17 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1458 1530 2742 2916 Size: 1458 72 1212 174 Fragments arranged by size: 1458 1212 174 72 BsrBI CCG'CTC Cuts at: 0 1275 1641 2916 Size: 1275 366 1275 Fragments arranged by size: 1275 1275 366 BsrDI GCAATG_nn' Cuts at: 0 53 640 1427 1821 2171 2916 Size: 53 587 787 394 350 745 Fragments arranged by size: 787 745 587 394 350 53 BsrFI r'CCGG_y Cuts at: 0 698 2916 Size: 698 2218 BssHII G'CGCG_C Cuts at: 0 1964 2916 Size: 1964 952 BssSI C'ACGA_G Cuts at: 0 1552 2731 2916 Size: 1552 1179 185 Fragments arranged by size: 1552 1179 185 BstAPI GCAn_nnn'nTGC Cuts at: 0 1473 2916 Size: 1473 1443 BstEII G'GTnAC_C Cuts at: 0 718 1705 2916 Size: 718 987 1211 Fragments arranged by size: 1211 987 718 BstXI CCAn_nnnn'nTGG Cuts at: 0 72 1859 2916 Size: 72 1787 1057 Fragments arranged by size: 1787 1057 72 BstYI r'GATC_y Cuts at: 0 373 423 731 2339 2630 2916 Size: 373 50 308 1608 291 286 Fragments arranged by size: 1608 373 308 291 286 50 Bsu36I CC'TnA_GG Cuts at: 0 227 2510 2916 Size: 227 2283 406 Fragments arranged by size: 2283 406 227 BtgI C'CryG_G Cuts at: 0 27 1398 1937 2916 Size: 27 1371 539 979 Fragments arranged by size: 1371 979 539 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 158 1212 2916 Size: 158 1054 1704 Fragments arranged by size: 1704 1054 158 BtsI GCAGTG_nn' Cuts at: 0 162 1095 1793 2719 2790 2916 Size: 162 933 698 926 71 126 Fragments arranged by size: 933 926 698 162 126 71 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1296 1331 2916 Size: 1296 35 1585 Fragments arranged by size: 1585 1296 35 DraI TTT'AAA Cuts at: 0 960 2916 Size: 960 1956 EaeI y'GGCC_r Cuts at: 0 77 1385 1907 2411 2916 Size: 77 1308 522 504 505 Fragments arranged by size: 1308 522 505 504 77 EagI C'GGCC_G Cuts at: 0 77 2916 Size: 77 2839 EarI CTCTTCn'nnn_ Cuts at: 0 1142 1835 2383 2793 2916 Size: 1142 693 548 410 123 Fragments arranged by size: 1142 693 548 410 123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 757 850 1859 2191 2728 2791 2878 Size: 415 342 93 1009 332 537 63 87 Cuts at: 2878 2916 Size: 38 Fragments arranged by size: 1009 537 415 342 332 93 87 63 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 799 2888 2916 Size: 799 2089 28 Fragments arranged by size: 2089 799 28 EcoO109I rG'GnC_Cy Cuts at: 0 38 949 950 1869 2298 2766 2916 Size: 38 911 1 919 429 468 150 Fragments arranged by size: 919 911 468 429 150 38 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 337 2916 Size: 337 2579 FspI TGC'GCA Cuts at: 0 2782 2916 Size: 2782 134 HaeII r_GCGC'y Cuts at: 0 876 1087 2243 2678 2916 Size: 876 211 1156 435 238 Fragments arranged by size: 1156 876 435 238 211 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 511 543 1033 1065 1264 1296 1747 1779 Size: 511 32 490 32 199 32 451 32 Cuts at: 1779 2916 Size: 1137 Fragments arranged by size: 1137 511 490 451 199 32 32 32 32 HincII GTy'rAC Cuts at: 0 2607 2916 Size: 2607 309 KasI G'GCGC_C Cuts at: 0 872 1083 2916 Size: 872 211 1833 Fragments arranged by size: 1833 872 211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 730 2802 2916 Size: 730 2072 114 Fragments arranged by size: 2072 730 114 MscI TGG'CCA Cuts at: 0 1387 1909 2916 Size: 1387 522 1007 Fragments arranged by size: 1387 1007 522 MslI CAynn'nnrTG Cuts at: 0 70 499 1498 2452 2698 2735 2916 Size: 70 429 999 954 246 37 181 Fragments arranged by size: 999 954 429 246 181 70 37 MspA1I CmG'CkG Cuts at: 0 54 1356 1688 1881 2316 2666 2856 2916 Size: 54 1302 332 193 435 350 190 60 Fragments arranged by size: 1302 435 350 332 193 190 60 54 NarI GG'CG_CC Cuts at: 0 873 1084 2916 Size: 873 211 1832 Fragments arranged by size: 1832 873 211 NcoI C'CATG_G Cuts at: 0 27 1398 1937 2916 Size: 27 1371 539 979 Fragments arranged by size: 1371 979 539 27 NruI TCG'CGA Cuts at: 0 2279 2916 Size: 2279 637 NsiI A_TGCA'T Cuts at: 0 2841 2916 Size: 2841 75 NspI r_CATG'y Cuts at: 0 87 250 696 1802 2256 2355 2587 2781 Size: 87 163 446 1106 454 99 232 194 Cuts at: 2781 2916 Size: 135 Fragments arranged by size: 1106 454 446 232 194 163 135 99 87 PflMI CCAn_nnn'nTGG Cuts at: 0 2769 2916 Size: 2769 147 PfoI T'CCnGG_A Cuts at: 0 431 734 2916 Size: 431 303 2182 Fragments arranged by size: 2182 431 303 PmlI CAC'GTG Cuts at: 0 771 2916 Size: 771 2145 PpuMI rG'GwC_Cy Cuts at: 0 2298 2916 Size: 2298 618 PshAI GACnn'nnGTC Cuts at: 0 340 2916 Size: 340 2576 PspOMI G'GGCC_C Cuts at: 0 38 949 2310 2916 Size: 38 911 1361 606 Fragments arranged by size: 1361 911 606 38 PstI C_TGCA'G Cuts at: 0 474 1541 1634 1997 2855 2916 Size: 474 1067 93 363 858 61 Fragments arranged by size: 1067 858 474 363 93 61 PvuII CAG'CTG Cuts at: 0 1356 1688 2666 2856 2916 Size: 1356 332 978 190 60 Fragments arranged by size: 1356 978 332 190 60 SalI G'TCGA_C Cuts at: 0 2605 2916 Size: 2605 311 SanDI GG'GwC_CC Cuts at: 0 2298 2916 Size: 2298 618 SbfI CC_TGCA'GG Cuts at: 0 1541 2916 Size: 1541 1375 SfcI C'TryA_G Cuts at: 0 470 1537 1630 1993 2851 2916 Size: 470 1067 93 363 858 65 Fragments arranged by size: 1067 858 470 363 93 65 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1933 2916 Size: 1933 983 SfoI GGC'GCC Cuts at: 0 874 1085 2916 Size: 874 211 1831 Fragments arranged by size: 1831 874 211 SmaI CCC'GGG Cuts at: 0 2335 2916 Size: 2335 581 SmlI C'TyrA_G Cuts at: 0 1315 2199 2916 Size: 1315 884 717 Fragments arranged by size: 1315 884 717 SphI G_CATG'C Cuts at: 0 250 1802 2355 2587 2781 2916 Size: 250 1552 553 232 194 135 Fragments arranged by size: 1552 553 250 232 194 135 StuI AGG'CCT Cuts at: 0 1441 2883 2916 Size: 1441 1442 33 Fragments arranged by size: 1442 1441 33 StyI C'CwwG_G Cuts at: 0 27 1101 1217 1292 1398 1436 1937 2184 Size: 27 1074 116 75 106 38 501 247 Cuts at: 2184 2916 Size: 732 Fragments arranged by size: 1074 732 501 247 116 106 75 38 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 901 943 2516 2776 2916 Size: 901 42 1573 260 140 Fragments arranged by size: 1573 901 260 140 42 TatI w'GTAC_w Cuts at: 0 941 2916 Size: 941 1975 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 207 239 1558 1590 2916 Size: 207 32 1319 32 1326 Fragments arranged by size: 1326 1319 207 32 32 Tth111I GACn'n_nGTC Cuts at: 0 552 565 1069 2011 2916 Size: 552 13 504 942 905 Fragments arranged by size: 942 905 552 504 13 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 69 1935 2115 2916 Size: 69 1866 180 801 Fragments arranged by size: 1866 801 180 69 XmaI C'CCGG_G Cuts at: 0 2333 2916 Size: 2333 583 XmnI GAAnn'nnTTC Cuts at: 0 998 2863 2916 Size: 998 1865 53 Fragments arranged by size: 1865 998 53 Enzymes that do cut: AarI AccI AclI AcuI AfeI AgeI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NruI NsiI NspI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SalI SanDI SbfI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AflII AflIII AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BmtI BplI BsaBI BsiWI BsmBI BspEI BspHI BsrGI BstBI BstZ17I ClaI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI PacI PasI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI