################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI ScaI SexAI SfiI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### Acc65I 883 GGTACC G^GTACC AccII 61 CGCG CG^CG AcyI 67 GGCGCC G[GA]^CG[CT]C Alw44I 709 GTGCAC G^TGCAC AocI 124 CCTGAGG CC^T[ACGT]AGG ApaLI 709 GTGCAC G^TGCAC Asp718I 883 GGTACC G^GTACC AspHI 709 GTGCAC G[AT]GC[AT]^C AvaI 53 CCCGGG C^[CT]CG[GA]G BamHI 835 GGATCC G^GATCC BbeI 67 GGCGCC GGCGC^C BbrPI 712 CACGTG CAC^GTG BbsI 253 GTCTTC GAAGAC(2/6) BcgI 525 CGAGGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 918 GGATAC GTATCC(6/5) BfaI 626 CTAG C^TAG BglI 1039 GCCGTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 253 GTCTTC GAAGAC(2/6) BsaAI 712 CACGTG [CT]AC^GT[GA] BsaHI 67 GGCGCC G[GA]^CG[CT]C BsiHKAI 709 GTGCAC G[AT]GC[AT]^C BsmBI 857 GAGACG CGTCTC(1/5) BsoBI 53 CCCGGG C^[CT]CG[GA]G BspHI 305 TCATGA T^CATGA BspMI 1101 GCAGGT ACCTGC(4/8) BsrBI 842 CCGCTC CCGCTC(-3/-3) BsrDI 893 GCAATG GCAATG(2/0) BstUI 61 CGCG CG^CG BstYI 835 GGATCC [GA]^GATC[CT] Bsu36I 124 CCTGAGG CC^T[ACGT]AGG DpnII 836 GATC ^GATC Eco81I 124 CCTGAGG CC^T[ACGT]AGG EcoNI 120 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 831 CCTTGG C^C[AT][AT]GG HaeII 67 GGCGCC [GA]GCGC^[CT] HgaI 692 GCGTC GACGC(5/10) Hin1I 67 GGCGCC G[GA]^CG[CT]C HphI 45 GGTGA GGTGA(8/7) KasI 67 GGCGCC G^GCGCC KpnI 883 GGTACC GGTAC^C KspI 60 CCGCGG CCGC^GG MaeI 626 CTAG C^TAG MaeIII 466 GTCAC ^GT[ACGT]AC MboI 836 GATC ^GATC MflI 835 GGATCC [GA]^GATC[CT] MseI 577 TTAA T^TAA MvnI 61 CGCG CG^CG NarI 67 GGCGCC GG^CGCC NdeII 836 GATC ^GATC NspI 210 GCATGC [GA]CATG^[CT] PmaCI 712 CACGTG CAC^GTG PmlI 712 CACGTG CAC^GTG PvuII 1089 CAGCTG CAG^CTG RcaI 305 TCATGA T^CATGA SacII 60 CCGCGG CCGC^GG SapI 664 GAAGAGC GCTCTTC(1/4) Sau3AI 836 GATC ^GATC SbfI 1098 CCTGCAGG CCTGCA^GG SfoI 67 GGCGCC GGC^GCC SmaI 53 CCCGGG CCC^GGG SphI 210 GCATGC GCATG^C Sse8387I 1098 CCTGCAGG CCTGCA^GG StyI 831 CCTTGG C^C[AT][AT]GG Tru9I 577 TTAA T^TAA Tsp45I 466 GTCAC ^GT[GC]AC XcmI 569 CCAACCTGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 835 GGATCC [GA]^GATC[CT] XmaCI 53 CCCGGG C^CCGGG XmaI 53 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 836 GATCC GGATC(4/5) 835 GGATC AlwNI 14 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 1132 CAGTGCCTG ApaI 604 GGGCCC GGGCC^C 971 GGGCCC BalI 299 TGGCCA TGG^CCA 1010 TGGCCA BsaI 31 GAGACC GGTCTC(1/5) 450 GGTCTC BseRI 127 GAGGAG GAGGAG(10/8) 652 GAGGAG BstAPI 559 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 634 GCAGAGTATGC BstBI 866 TTCGAA TT^CGAA 904 TTCGAA BstXI 35 CCACTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 134 CCAAACCTGTGG CfoI 68 GCGC GCG^C 1140 GCGC DsaI 60 CCGCGG C^C[GA][CT]GG 618 CCACGG EaeI 299 TGGCCA [CT]^GGCC[GA] 1010 TGGCCA HhaI 68 GCGC GCG^C 1140 GCGC HinP1I 68 GCGC G^CGC 1140 GCGC MaeII 713 ACGT A^CGT 860 ACGT MluNI 299 TGGCCA TGG^CCA 1010 TGGCCA MscI 299 TGGCCA TGG^CCA 1010 TGGCCA NspV 866 TTCGAA TT^CGAA 904 TTCGAA PflMI 13 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 321 CCACTTGCTGG PpuMI 170 GGGACCC [GA]G^G[AT]CC[CT] 722 GGGACCT PstI 730 CTGCAG CTGCA^G 1099 CTGCAG SfcI 730 CTGCAG C^T[GA][CT]AG 1099 CTGCAG SfuI 866 TTCGAA TT^CGAA 904 TTCGAA TfiI 869 GAATC G^A[AT]TC 1082 GATTC Van91I 13 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 321 CCACTTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I