Restriction map for
pFN21AE6232
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 98 CGCG CG^CG
AcsI 874 AAATTC [GA]^AATT[CT]
AfaI 819 GTAC GT^AC
AlwI 322 GATCC GGATC(4/5)
AlwNI 758 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 84 CCTCAGG CC^T[ACGT]AGG
ApaI 53 GGGCCC GGGCC^C
ApoI 874 AAATTC [GA]^AATT[CT]
AspHI 753 GAGCAC G[AT]GC[AT]^C
AspI 421 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 437 CCCGGG C^[CT]CG[GA]G
BalI 704 TGGCCA TGG^CCA
BlpI 298 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 298 GCTGAGC GC^T[ACGT]AGC
BsaAI 881 TACGTG [CT]AC^GT[GA]
BsgI 501 CTGCAC GTGCAG(16/14)
BsiHKAI 753 GAGCAC G[AT]GC[AT]^C
BsoBI 437 CCCGGG C^[CT]CG[GA]G
BspMI 237 GCAGGT ACCTGC(4/8)
BstUI 98 CGCG CG^CG
BstXI 287 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 84 CCTCAGG CC^T[ACGT]AGG
CelII 298 GCTGAGC GC^T[ACGT]AGC
DpnII 322 GATC ^GATC
DraIII 890 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 508 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 413 CCATGG C^C[GA][CT]GG
Eco81I 84 CCTCAGG CC^T[ACGT]AGG
HaeII 64 AGCGCC [GA]GCGC^[CT]
MaeII 882 ACGT A^CGT
MboI 322 GATC ^GATC
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
MseI 869 TTAA T^TAA
MspA1I 22 CAGCTG C[AC]G^C[GT]G
MvnI 98 CGCG CG^CG
NcoI 413 CCATGG C^CATGG
NdeII 322 GATC ^GATC
PflFI 421 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 22 CAGCTG CAG^CTG
RsaI 819 GTAC GT^AC
Sau3AI 322 GATC ^GATC
SmaI 437 CCCGGG CCC^GGG
TaqI 687 TCGA T^CGA
Tru9I 869 TTAA T^TAA
Tsp509I 875 AATT ^AATT
Tth111I 421 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 687 TCGA T^CGA
XcmI 103 CCAGGGGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 437 CCCGGG C^CCGGG
XmaI 437 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BanII 53 GGGCCC G[GA]GC[CT]^C
724 GAGCCC
BsaI 689 GAGACC GGTCTC(1/5)
90 GGTCTC
BsmAI 689 GAGAC GTCTC(1/5)
91 GTCTC
BsrI 677 ACTGG ACTGG(1/-1)
355 CCAGT
EaeI 59 CGGCCA [CT]^GGCC[GA]
704 TGGCCA
EcoT14I 386 CCAAGG C^C[AT][AT]GG
413 CCATGG
HgaI 80 GCGTC GACGC(5/10)
246 GCGTC
HphI 618 GGTGA GGTGA(8/7)
518 TCACC
MslI 204 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CATTGGGATG
NlaIII 233 CATG CATG^
414 CATG
PpuMI 191 GGGTCCT [GA]G^G[AT]CC[CT]
768 AGGACCC
SexAI 829 ACCTGGT A^CC[AT]GGT
891 ACCTGGT
StyI 386 CCAAGG C^C[AT][AT]GG
413 CCATGG
TfiI 561 GAATC G^A[AT]TC
710 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboII
MnlI MspI MvaI MwoI NciI
NlaIV PleI PstI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI