(Circular) (Six-base) MAPSORT of: pFN21AE6232.seq Check: 8142 from: 1 to: 5605 pFN21AE6232 5605 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 3668 5052 5320 1632 Size: 542 1494 1384 268 1917 Fragments arranged by size: 1917 1494 1384 542 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 2768 3348 4920 2768 Size: 580 1572 3453 Fragments arranged by size: 3453 1572 580 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 5605 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2879 2914 3140 3292 5401 1493 Size: 1386 35 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2442 2930 1870 Size: 73 499 488 4545 Fragments arranged by size: 4545 499 488 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2733 2924 1391 Size: 671 671 191 4072 Fragments arranged by size: 4072 671 671 191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4216 136 Size: 107 122 71 631 605 337 2207 1525 Fragments arranged by size: 2207 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 2304 3022 2304 Size: 718 4887 Bme1580I G_kGCm'C Cuts at: 1790 2062 3666 4822 1790 Size: 272 1604 1156 2573 Fragments arranged by size: 2573 1604 1156 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2140 2369 2790 4265 1239 Size: 859 42 229 421 1475 2579 Fragments arranged by size: 2579 1475 859 421 229 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2101 2688 4268 882 Size: 617 602 587 1580 2219 Fragments arranged by size: 2219 1580 617 602 587 BsaAI yAC'GTr Cuts at: 493 1204 2888 3396 493 Size: 711 1684 508 2702 Fragments arranged by size: 2702 1684 711 508 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2195 2452 2455 1186 Size: 909 100 257 3 4336 Fragments arranged by size: 4336 909 257 100 3 BseYI C'CCAG_C Cuts at: 2029 2050 4808 5304 2029 Size: 21 2758 496 2330 Fragments arranged by size: 2758 2330 496 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2491 1163 Size: 573 755 4277 Fragments arranged by size: 4277 755 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2762 3666 3751 4822 2762 Size: 904 85 1071 3545 Fragments arranged by size: 3545 1071 904 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2733 2762 2924 3666 3751 4822 Size: 399 272 671 29 162 742 85 1071 Cuts at: 4822 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 671 399 272 162 85 29 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 2960 844 Size: 1389 727 3489 Fragments arranged by size: 3489 1389 727 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstXI CCAn_nnnn'nTGG Cuts at: 2299 2299 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600 Size: 963 367 385 17 1452 86 12 346 Cuts at: 5600 1972 Size: 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2090 2090 Size: 5605 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3700 Fragments arranged by size: 3700 939 552 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3121 3915 3935 2503 Size: 618 794 20 4173 Fragments arranged by size: 4173 794 618 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2900 2900 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2519 4612 809 Size: 507 1203 2093 1802 Fragments arranged by size: 2093 1802 1203 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2709 3052 Size: 54 1425 50 275 18 234 645 343 Cuts at: 3052 3941 5570 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 645 343 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3535 1353 Size: 2182 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2140 2174 2369 2790 3668 4265 Size: 393 466 42 34 195 421 878 597 Cuts at: 4265 5052 5320 1239 Size: 787 268 1524 Fragments arranged by size: 1524 878 787 597 466 421 393 268 195 42 34 EcoO109I rG'GnC_Cy Cuts at: 2059 2197 2481 2774 2059 Size: 138 284 293 4890 Fragments arranged by size: 4890 293 284 138 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2073 4752 1678 Size: 395 2679 2531 Fragments arranged by size: 2679 2531 395 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 2511 2543 4246 Size: 32 135 32 701 32 206 32 1703 Cuts at: 4246 4278 4320 4352 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1703 701 206 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2234 2265 4719 4903 1305 Size: 929 31 2454 184 2007 Fragments arranged by size: 2454 2007 929 184 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 2904 Fragments arranged by size: 2904 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2618 3563 3922 4081 518 Size: 648 1047 405 945 359 159 2042 Fragments arranged by size: 2042 1047 945 648 405 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 3021 3698 4846 5091 1606 Size: 423 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3700 Fragments arranged by size: 3700 939 552 414 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 2108 1348 Size: 760 4845 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2433 2433 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2532 2564 3674 3706 5187 5219 2532 Size: 32 1110 32 1481 32 2918 Fragments arranged by size: 2918 1481 1110 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2774 2197 Size: 577 5028 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 5605 PstI C_TGCA'G Cuts at: 830 2149 2823 2854 2957 830 Size: 1319 674 31 103 3478 Fragments arranged by size: 3478 1319 674 103 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2029 2029 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SexAI A'CCwGG_T Cuts at: 2834 2896 2834 Size: 62 5543 SfcI C'TryA_G Cuts at: 826 1045 2145 2819 2850 2953 4088 4769 4960 Size: 219 1100 674 31 103 1135 681 191 Cuts at: 4960 826 Size: 1471 Fragments arranged by size: 1471 1135 1100 681 674 219 191 103 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2444 2932 1872 Size: 572 488 4545 Fragments arranged by size: 4545 572 488 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 2418 3044 513 Size: 552 939 387 27 626 3074 Fragments arranged by size: 3074 939 626 552 387 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3632 3649 3802 3987 4432 325 Size: 1255 2052 17 153 185 445 1498 Fragments arranged by size: 2052 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3851 5563 Size: 274 80 33 51 1443 846 1028 1712 Cuts at: 5563 96 Size: 138 Fragments arranged by size: 1712 1443 1028 846 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2884 2916 5565 5597 2884 Size: 32 2649 32 2892 Fragments arranged by size: 2892 2649 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2429 1316 Size: 1113 4492 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2115 2011 Size: 104 5501 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2442 2930 1870 Size: 572 488 4545 Fragments arranged by size: 4545 572 488 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI