(Linear) (Six-base) MAPSORT of: KIEE6232.seq Check: 514 from: 1 to: 900 KIEE6232.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 900 Size: 169 731 AlwNI CAG_nnn'CTG Cuts at: 0 763 900 Size: 763 137 ApaI G_GGCC'C Cuts at: 0 57 900 Size: 57 843 ApoI r'AATT_y Cuts at: 0 874 900 Size: 874 26 AvaI C'yCGr_G Cuts at: 0 437 900 Size: 437 463 BanII G_rGCy'C Cuts at: 0 57 728 900 Size: 57 671 172 Fragments arranged by size: 671 172 57 BlpI GC'TnA_GC Cuts at: 0 299 900 Size: 299 601 Bme1580I G_kGCm'C Cuts at: 0 57 900 Size: 57 843 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 135 364 785 900 Size: 93 42 229 421 115 Fragments arranged by size: 421 229 115 93 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 96 683 900 Size: 96 587 217 Fragments arranged by size: 587 217 96 BsaAI yAC'GTr Cuts at: 0 883 900 Size: 883 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 90 190 447 450 900 Size: 90 100 257 3 450 Fragments arranged by size: 450 257 100 90 3 BseYI C'CCAG_C Cuts at: 0 24 45 900 Size: 24 21 855 Fragments arranged by size: 855 24 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 486 900 Size: 486 414 BsiHKAI G_wGCw'C Cuts at: 0 757 900 Size: 757 143 Bsp1286I G_dGCh'C Cuts at: 0 57 728 757 900 Size: 57 671 29 143 Fragments arranged by size: 671 143 57 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 228 900 Size: 228 672 BstXI CCAn_nnnn'nTGG Cuts at: 0 294 900 Size: 294 606 Bsu36I CC'TnA_GG Cuts at: 0 85 900 Size: 85 815 BtgI C'CryG_G Cuts at: 0 413 900 Size: 413 487 BtsI GCAGTG_nn' Cuts at: 0 498 900 Size: 498 402 DraIII CAC_nnn'GTG Cuts at: 0 895 900 Size: 895 5 DrdI GACnn_nn'nnGTC Cuts at: 0 514 900 Size: 514 386 EaeI y'GGCC_r Cuts at: 0 59 704 900 Size: 59 645 196 Fragments arranged by size: 645 196 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 135 169 364 785 900 Size: 93 42 34 195 421 115 Fragments arranged by size: 421 195 115 93 42 34 EcoO109I rG'GnC_Cy Cuts at: 0 54 192 476 769 900 Size: 54 138 284 293 131 Fragments arranged by size: 293 284 138 131 54 HaeII r_GCGC'y Cuts at: 0 68 900 Size: 68 832 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 268 300 506 538 900 Size: 268 32 206 32 362 Fragments arranged by size: 362 268 206 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 229 260 900 Size: 229 31 640 Fragments arranged by size: 640 229 31 MscI TGG'CCA Cuts at: 0 706 900 Size: 706 194 MslI CAynn'nnrTG Cuts at: 0 208 613 900 Size: 208 405 287 Fragments arranged by size: 405 287 208 MspA1I CmG'CkG Cuts at: 0 24 900 Size: 24 876 NcoI C'CATG_G Cuts at: 0 413 900 Size: 413 487 PasI CC'CwG_GG Cuts at: 0 103 900 Size: 103 797 PfoI T'CCnGG_A Cuts at: 0 428 900 Size: 428 472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 527 559 900 Size: 527 32 341 Fragments arranged by size: 527 341 32 PpuMI rG'GwC_Cy Cuts at: 0 192 769 900 Size: 192 577 131 Fragments arranged by size: 577 192 131 PspOMI G'GGCC_C Cuts at: 0 53 900 Size: 53 847 PstI C_TGCA'G Cuts at: 0 144 818 849 900 Size: 144 674 31 51 Fragments arranged by size: 674 144 51 31 PvuII CAG'CTG Cuts at: 0 24 900 Size: 24 876 SexAI A'CCwGG_T Cuts at: 0 829 891 900 Size: 829 62 9 Fragments arranged by size: 829 62 9 SfcI C'TryA_G Cuts at: 0 140 814 845 900 Size: 140 674 31 55 Fragments arranged by size: 674 140 55 31 SmaI CCC'GGG Cuts at: 0 439 900 Size: 439 461 StyI C'CwwG_G Cuts at: 0 386 413 900 Size: 386 27 487 Fragments arranged by size: 487 386 27 TatI w'GTAC_w Cuts at: 0 818 900 Size: 818 82 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 879 900 Size: 879 21 Tth111I GACn'n_nGTC Cuts at: 0 424 900 Size: 424 476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 110 900 Size: 110 790 XmaI C'CCGG_G Cuts at: 0 437 900 Size: 437 463 Enzymes that do cut: AcuI AlwNI ApaI ApoI AvaI BanII BlpI Bme1580I BpmI BsaI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstXI Bsu36I BtgI BtsI DraIII DrdI EaeI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI PasI PfoI PpiI PpuMI PspOMI PstI PvuII SexAI SfcI SmaI StyI TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XhoI XmnI ZraI