Restriction map for
pFN21AB5789
(Circular) (Six-base) MAPSORT of: pFN21AB5789.seq Check: 1213 from: 1 to: 7192
pFN21AB5789 7192 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5002 278
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4535 1937
Size: 2598 4594
Acc65I G'GTAC_C
Cuts at: 2417 4513 2417
Size: 2096 5096
AclI AA'CG_TT
Cuts at: 1563 5319 5692 1563
Size: 3756 373 3063
Fragments arranged by size:
3756 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2178 2439 3084 3177 3274 4499 5255 6639
Size: 546 261 645 93 97 1225 756 1384
Cuts at: 6639 6907 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1225 756 645 546 268 261
97 93
AfeI AGC'GCT
Cuts at: 3946 3946
Size: 7192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6995
AflIII A'CryG_T
Cuts at: 1203 2314 2538 3299 6091 7068 1203
Size: 1111 224 761 2792 977 1327
Fragments arranged by size:
2792 1327 1111 977 761 224
AgeI A'CCGG_T
Cuts at: 6023 6023
Size: 7192
AhdI GACnn_n'nnGTC
Cuts at: 5921 5921
Size: 7192
AleI CACnn'nnGTG
Cuts at: 1166 2717 2793 1166
Size: 1551 76 5565
Fragments arranged by size:
5565 1551 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4212 4244 4212
Size: 32 7160
AlwNI CAG_nnn'CTG
Cuts at: 2228 3062 3136 3524 3546 3638 3871 4270 4935
Size: 834 74 388 22 92 233 399 665
Cuts at: 4935 6507 2228
Size: 1572 2913
Fragments arranged by size:
2913 1572 834 665 399 388 233 92
74 22
ApaI G_GGCC'C
Cuts at: 2207 2254 2207
Size: 47 7145
ApaLI G'TGCA_C
Cuts at: 2345 2807 3854 5249 6405 2345
Size: 462 1047 1395 1156 3132
Fragments arranged by size:
3132 1395 1156 1047 462
ApoI r'AATT_y
Cuts at: 1493 2110 2932 3839 4501 4727 4879 6988 1493
Size: 617 822 907 662 226 152 2109 1697
Fragments arranged by size:
2109 1697 907 822 662 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5746 160
Size: 872 4714 1606
Fragments arranged by size:
4714 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
AvaI C'yCGr_G
Cuts at: 1870 1943 2833 3398 3440 4517 1870
Size: 73 890 565 42 1077 4545
Fragments arranged by size:
4545 1077 890 565 73 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3051 3084 3051
Size: 33 7159
BamHI G'GATC_C
Cuts at: 2218 4522 4889 2218
Size: 2304 367 4521
Fragments arranged by size:
4521 2304 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 2417 3878 4152 4379
Size: 325 217 627 584 46 1461 274 227
Cuts at: 4379 4513 5968 618
Size: 134 1455 1842
Fragments arranged by size:
1842 1461 1455 627 584 325 274 227
217 134 46
BanII G_rGCy'C
Cuts at: 1391 2207 2254 2404 2569 2929 3048 3143 3399
Size: 816 47 150 165 360 119 95 256
Cuts at: 3399 3447 4511 1391
Size: 48 1064 4072
Fragments arranged by size:
4072 1064 816 360 256 165 150 119
95 48 47
BbeI G_GCGC'C
Cuts at: 3882 4156 3882
Size: 274 6918
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6221
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 7192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2933 2967 5383 5417 1903
Size: 34 996 34 2416 34 3678
Fragments arranged by size:
3678 2416 996 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3916 5084 6294 3916
Size: 1168 1210 4814
Fragments arranged by size:
4814 1210 1168
BclI T'GATC_A
Cuts at: 1548 3334 1548
Size: 1786 5406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5803 136
Size: 107 122 71 631 605 4131 1525
Fragments arranged by size:
4131 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7187 7187
Size: 7192
BlpI GC'TnA_GC
Cuts at: 2408 4609 2408
Size: 2201 4991
Bme1580I G_kGCm'C
Cuts at: 1790 2207 2254 2349 2376 2811 3736 3858 4017
Size: 417 47 95 27 435 925 122 159
Cuts at: 4017 4101 4476 5253 6409 1790
Size: 84 375 777 1156 2573
Fragments arranged by size:
2573 1156 925 777 435 417 375 159
122 95 84 47 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3859 4923 5881
Size: 416 53 83 399 24 2442 1064 958
Cuts at: 5881 442
Size: 1753
Fragments arranged by size:
2442 1753 1064 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 3538 3859 2532
Size: 1006 321 5865
Fragments arranged by size:
5865 1006 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2502 2570 2633 2812 2840 2900 2984
Size: 1079 184 68 63 179 28 60 84
Cuts at: 2984 3660 4142 5852 1239
Size: 676 482 1710 2579
Fragments arranged by size:
2579 1710 1079 676 482 184 179 84
68 63 60 28
Bpu10I CC'TnA_GC
Cuts at: 2507 2576 3042 3137 3144 2507
Size: 69 466 95 7 6555
Fragments arranged by size:
6555 466 95 69 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2370 2468 5317 6182 6480 2370
Size: 98 2849 865 298 3082
Fragments arranged by size:
3082 2849 865 298 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5855 882
Size: 617 4356 2219
Fragments arranged by size:
4356 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 2872 2974 3300 4217 4983 493
Size: 711 1146 522 102 326 917 766 2702
Fragments arranged by size:
2702 1146 917 766 711 522 326 102
BsaBI GATnn'nnATC
Cuts at: 7186 7186
Size: 7192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3879 4153 4999 5381
Size: 53 83 186 936 2346 274 846 382
Cuts at: 5381 6016 275
Size: 635 1451
Fragments arranged by size:
2346 1451 936 846 635 382 274 186
83 53
BsaWI w'CCGG_w
Cuts at: 5625 6023 6297 6444 6855 5625
Size: 398 274 147 411 5962
Fragments arranged by size:
5962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2785 2815 4295 4325 1178
Size: 30 165 30 1382 30 1480 30 4045
Fragments arranged by size:
4045 1480 1382 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 1186
Size: 1093 6099
BseYI C'CCAG_C
Cuts at: 2064 2523 2560 3208 3736 3882 3991 4070 6395
Size: 459 37 648 528 146 109 79 2325
Cuts at: 6395 6891 2064
Size: 496 2365
Fragments arranged by size:
2365 2325 648 528 496 459 146 109
79 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 3620 3936 4015 1163
Size: 573 1218 666 316 79 4340
Fragments arranged by size:
4340 1218 666 573 316 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4642 5403 6431 1540
Size: 275 186 2641 761 1028 2301
Fragments arranged by size:
2641 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 2404 2594 2714 2811 3048 3219 3858 5253
Size: 55 190 120 97 237 171 639 1395
Cuts at: 5253 5338 6409 2349
Size: 85 1071 3132
Fragments arranged by size:
3132 1395 1071 639 237 190 171 120
97 85 55
BsmI GAATG_Cn'
Cuts at: 2857 4703 4796 2857
Size: 1846 93 5253
Fragments arranged by size:
5253 1846 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2254 2349 2376 2404 2569 2594
Size: 399 417 47 95 27 28 165 25
Cuts at: 2594 2714 2811 2929 3048 3143 3219 3399 3447
Size: 120 97 118 119 95 76 180 48
Cuts at: 3447 3736 3858 4017 4101 4476 4511 5253 5338
Size: 289 122 159 84 375 35 742 85
Cuts at: 5338 6409 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 417 399 375 289 180
165 159 122 120 119 118 97 95
95 85 84 76 48 47 35 28
27 25
BspEI T'CCGG_A
Cuts at: 6855 6855
Size: 7192
BspHI T'CATG_A
Cuts at: 3580 5081 6811 3580
Size: 1501 1730 3961
Fragments arranged by size:
3961 1730 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2919 4547 844
Size: 2075 1628 3489
Fragments arranged by size:
3489 2075 1628
BsrBI CCG'CTC
Cuts at: 1394 1823 5079 1394
Size: 429 3256 3507
Fragments arranged by size:
3507 3256 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5687 5861 65
Size: 1183 4439 174 1396
Fragments arranged by size:
4439 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3740 5836 6023 1700
Size: 110 1930 2096 187 2869
Fragments arranged by size:
2869 2096 1930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7192
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7192
BssSI C'ACGA_G
Cuts at: 5252 6264 5252
Size: 1012 6180
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3089 4315 794
Size: 2295 1226 3671
Fragments arranged by size:
3671 2295 1226
BstBI TT'CG_AA
Cuts at: 5996 5996
Size: 7192
BstEII G'GTnAC_C
Cuts at: 1184 2242 2290 2654 1184
Size: 1058 48 364 5722
Fragments arranged by size:
5722 1058 364 48
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2884 3268 4049 2254
Size: 630 384 781 5397
Fragments arranged by size:
5397 781 630 384
BstYI r'GATC_y
Cuts at: 1972 2218 2488 4522 4889 5274 5291 6743 6829
Size: 246 270 2034 367 385 17 1452 86
Cuts at: 6829 6841 7187 1972
Size: 12 346 1977
Fragments arranged by size:
2034 1977 1452 385 367 346 270 246
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2665 3150 3384 3791 2665
Size: 485 234 407 6066
Fragments arranged by size:
6066 485 407 234
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 2938 3261 3651 4048 4134
Size: 552 939 684 250 323 390 397 86
Cuts at: 4134 4233 513
Size: 99 3472
Fragments arranged by size:
3472 939 684 552 397 390 323 250
99 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6453
Fragments arranged by size:
6453 683 56
BtsI GCAGTG_nn'
Cuts at: 2679 3205 4316 4368 4708 5502 5522 2679
Size: 526 1111 52 340 794 20 4349
Fragments arranged by size:
4349 1111 794 526 340 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7157
DraI TTT'AAA
Cuts at: 4496 4496
Size: 7192
DraIII CAC_nnn'GTG
Cuts at: 3124 3124
Size: 7192
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3029 3076 6199 809
Size: 507 1713 47 3123 1802
Fragments arranged by size:
3123 1802 1713 507 47
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3258 3411 4089
Size: 54 1425 50 275 18 1428 153 678
Cuts at: 4089 4639 5528 7157 8
Size: 550 889 1629 43
Fragments arranged by size:
1629 1428 1425 889 678 550 275 153
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4639 1537
Size: 275 2827 4090
Fragments arranged by size:
4090 2827 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 5122 1353
Size: 1094 2675 3423
Fragments arranged by size:
3423 2675 1094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3443 5779 6153 6299 387
Size: 1064 138 1854 2336 374 146 1280
Fragments arranged by size:
2336 1854 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2402 3046 2402
Size: 644 6548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2318 2439 2502 2570 2633 2812
Size: 393 546 140 121 63 68 63 179
Cuts at: 2812 2840 2900 2984 3084 3177 3274 3660 4142
Size: 28 60 84 100 93 97 386 482
Cuts at: 4142 4499 5255 5852 6639 6907 1239
Size: 357 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 546 482 393 386
357 268 179 140 121 100 97 93
84 68 63 63 60 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2510 2915 3383 2510
Size: 405 468 6319
Fragments arranged by size:
6319 468 405
EcoO109I rG'GnC_Cy
Cuts at: 2203 2250 2251 2619 2837 3635 3979 4078 4447
Size: 47 1 368 218 798 344 99 369
Cuts at: 4447 2203
Size: 4948
Fragments arranged by size:
4948 798 369 368 344 218 99 47
1
EcoRI G'AATT_C
Cuts at: 2932 4501 4879 2932
Size: 1569 378 5245
Fragments arranged by size:
5245 1569 378
EcoRV GAT'ATC
Cuts at: 3328 3328
Size: 7192
FspI TGC'GCA
Cuts at: 4319 5698 4319
Size: 1379 5813
HaeII r_GCGC'y
Cuts at: 1678 3882 3948 4156 6339 1678
Size: 2204 66 208 2183 2531
Fragments arranged by size:
2531 2204 2183 208 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2487 2519 2648 2680 3528
Size: 32 135 32 915 32 129 32 848
Cuts at: 3528 3560 4273 4305 5833 5865 5907 5939 1373
Size: 32 713 32 1528 32 42 32 2626
Fragments arranged by size:
2626 1528 915 848 713 135 129 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4536 4782 669
Size: 988 281 2598 246 3079
Fragments arranged by size:
3079 2598 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7192
HpaI GTT'AAC
Cuts at: 4782 4782
Size: 7192
KasI G'GCGC_C
Cuts at: 3878 4152 3878
Size: 274 6918
KpnI G_GTAC'C
Cuts at: 2421 4517 2421
Size: 2096 5096
MfeI C'AATT_G
Cuts at: 4791 4791
Size: 7192
MluI A'CGCG_T
Cuts at: 7068 7068
Size: 7192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3675 3795 6306 6490 1305
Size: 2370 120 2511 184 2007
Fragments arranged by size:
2511 2370 2007 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3260 3413 4091 10
Size: 54 1425 343 1428 153 678 3111
Fragments arranged by size:
3111 1428 1425 678 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2717 2793 5150 5509 5668 518
Size: 648 1551 76 2357 359 159 2042
Fragments arranged by size:
2357 2042 1551 648 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 2140 2548 2629 4608 5285 6433 6678 1606
Size: 534 408 81 1979 677 1148 245 2120
Fragments arranged by size:
2120 1979 1148 677 534 408 245 81
NaeI GCC'GGC
Cuts at: 3742 3742
Size: 7192
NarI GG'CG_CC
Cuts at: 3879 4153 3879
Size: 274 6918
NcoI C'CATG_G
Cuts at: 513 1065 2004 2938 3261 4048 513
Size: 552 939 934 323 787 3657
Fragments arranged by size:
3657 939 934 787 552 323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7192
NgoMIV G'CCGG_C
Cuts at: 3740 3740
Size: 7192
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7192
NotI GC'GGCC_GC
Cuts at: 4639 4639
Size: 7192
NspI r_CATG'y
Cuts at: 1132 1229 2318 2542 3533 4318 4345 4550 6095
Size: 97 1089 224 991 785 27 205 1545
Cuts at: 6095 1132
Size: 2229
Fragments arranged by size:
2229 1545 1089 991 785 224 205 97
27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7192
PciI A'CATG_T
Cuts at: 2314 2538 6091 2314
Size: 224 3553 3415
Fragments arranged by size:
3553 3415 224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2228 2611 2632 3524 3638 1108
Size: 1120 383 21 892 114 4662
Fragments arranged by size:
4662 1120 892 383 114 21
PmeI GTTT'AAAC
Cuts at: 4496 4496
Size: 7192
PmlI CAC'GTG
Cuts at: 2350 2974 3300 4217 2350
Size: 624 326 917 5325
Fragments arranged by size:
5325 917 624 326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5261 5293 6774 6806 5261
Size: 32 1481 32 5647
Fragments arranged by size:
5647 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2619 2837 4078 4447 2619
Size: 218 1241 369 5364
Fragments arranged by size:
5364 1241 369 218
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7192
PsiI TTA'TAA
Cuts at: 4762 4762
Size: 7192
PspOMI G'GGCC_C
Cuts at: 2203 2250 2203
Size: 47 7145
PspXI vC'TCGA_Gb
Cuts at: 3440 3440
Size: 7192
PstI C_TGCA'G
Cuts at: 830 2074 3876 4544 830
Size: 1244 1802 668 3478
Fragments arranged by size:
3478 1802 1244 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7192
PvuII CAG'CTG
Cuts at: 2548 2629 2548
Size: 81 7111
SacI G_AGCT'C
Cuts at: 2404 3048 2404
Size: 644 6548
SalI G'TCGA_C
Cuts at: 1936 4534 1936
Size: 2598 4594
SanDI GG'GwC_CC
Cuts at: 2837 2837
Size: 7192
SbfI CC_TGCA'GG
Cuts at: 3876 4544 3876
Size: 668 6524
ScaI AGT'ACT
Cuts at: 5440 7152 5440
Size: 1712 5480
SexAI A'CCwGG_T
Cuts at: 2367 3055 2367
Size: 688 6504
SfcI C'TryA_G
Cuts at: 826 1045 2070 2285 2395 3872 4108 4540 5675
Size: 219 1025 215 110 1477 236 432 1135
Cuts at: 5675 6356 6547 826
Size: 681 191 1471
Fragments arranged by size:
1477 1471 1135 1025 681 432 236 219
215 191 110
SfoI GGC'GCC
Cuts at: 3880 4154 3880
Size: 274 6918
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7192
SmaI CCC'GGG
Cuts at: 1872 2835 3400 4519 1872
Size: 963 565 1119 4545
Fragments arranged by size:
4545 1119 963 565
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2385 2447 3440 5296 6197 6459
Size: 197 926 442 62 993 1856 901 262
Cuts at: 6459 820
Size: 1553
Fragments arranged by size:
1856 1553 993 926 901 442 262 197
62
SnaBI TAC'GTA
Cuts at: 493 4983 493
Size: 4490 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7192
SphI G_CATG'C
Cuts at: 1132 4318 4345 4550 1132
Size: 3186 27 205 3774
Fragments arranged by size:
3774 3186 205 27
SspI AAT'ATT
Cuts at: 5 52 5116 5
Size: 47 5064 2081
Fragments arranged by size:
5064 2081 47
StuI AGG'CCT
Cuts at: 1517 2425 2663 1517
Size: 908 238 6046
Fragments arranged by size:
6046 908 238
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2583 2938 3261 4048 4120
Size: 552 939 54 525 355 323 787 72
Cuts at: 4120 4191 4631 513
Size: 71 440 3074
Fragments arranged by size:
3074 939 787 552 525 440 355 323
72 71 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2274 2642 2707 4195 4436 5219 5236
Size: 1255 694 368 65 1488 241 783 17
Cuts at: 5236 5389 5574 6019 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1488 1255 783 694 445 368 241
185 153 65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5438 7150 96
Size: 274 80 33 51 1443 3461 1712 138
Fragments arranged by size:
3461 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2785 2817 4108 4140 4295 4327 7152 7184 2785
Size: 32 1291 32 155 32 2825 32 2793
Fragments arranged by size:
2825 2793 1291 155 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4432 1316
Size: 3116 4076
XbaI T'CTAG_A
Cuts at: 4528 4528
Size: 7192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 4166 2686
Size: 1480 5712
XhoI C'TCGA_G
Cuts at: 1943 3440 1943
Size: 1497 5695
XmaI C'CCGG_G
Cuts at: 1870 2833 3398 4517 1870
Size: 963 565 1119 4545
Fragments arranged by size:
4545 1119 963 565
XmnI GAAnn'nnTTC
Cuts at: 5321 5321
Size: 7192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5000 276
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BfrBI BsiWI BsmBI BstZ17I FalI
FseI FspAI NruI NsiI PacI PfoI PsrI RsrII
SacII SapI SfiI SrfI SwaI