################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BanII BbrPI BbsI BcgI BclI BfaI BfrI BglI BlnI BmyI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 235 GGCGCC G[GA]^CG[CT]C AfaI 420 GTAC GT^AC AspI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 150 CCCGGG C^[CT]CG[GA]G BbeI 235 GGCGCC GGCGC^C BbvCI 102 CCTCAGC CCTCAGC(-5/-2) BbvI 258 GCTGC GCAGC(8/12) BciVI 397 GTATCC GTATCC(6/5) BglII 50 AGATCT A^GATCT BlpI 249 GCTGAGC GC^T[ACGT]AGC BpmI 88 CTGGAG CTGGAG(16/14) Bpu1102I 249 GCTGAGC GC^T[ACGT]AGC BsaHI 235 GGCGCC G[GA]^CG[CT]C BsoBI 150 CCCGGG C^[CT]CG[GA]G BspMI 143 ACCTGC ACCTGC(4/8) BstEII 321 GGTCACC G^GT[ACGT]ACC BstF5I 270 CATCC GGATG(2/0) BstPI 321 GGTCACC G^GT[ACGT]ACC BstXI 182 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 50 AGATCT [GA]^GATC[CT] CelII 249 GCTGAGC GC^T[ACGT]AGC DpnII 51 GATC ^GATC DsaI 197 CCGTGG C^C[GA][CT]GG EagI 356 CGGCCG C^GGCCG EclXI 356 CGGCCG C^GGCCG Eco52I 356 CGGCCG C^GGCCG EcoO65I 321 GGTCACC G^GT[ACGT]ACC EcoT14I 182 CCAAGG C^C[AT][AT]GG FokI 270 CATCC GGATG(9/13) HaeII 235 GGCGCC [GA]GCGC^[CT] HgaI 205 GACGC GACGC(5/10) Hin1I 235 GGCGCC G[GA]^CG[CT]C HincII 289 GTCAAC GT[CT]^[GA]AC HindII 289 GTCAAC GT[CT]^[GA]AC HinfI 84 GACTC G^A[ACGT]TC KasI 235 GGCGCC G^GCGCC MaeIII 322 GTCAC ^GT[ACGT]AC MboI 51 GATC ^GATC MflI 50 AGATCT [GA]^GATC[CT] MslI 27 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 235 GGCGCC GG^CGCC NdeII 51 GATC ^GATC NlaIII 39 CATG CATG^ PflFI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 84 GACTC GAGTC(4/5) RsaI 420 GTAC GT^AC Sau3AI 51 GATC ^GATC SfaNI 218 GCATC GCATC(5/9) SfoI 235 GGCGCC GGC^GCC SmaI 150 CCCGGG CCC^GGG StyI 182 CCAAGG C^C[AT][AT]GG TseI 258 GCTGC G^C[AT]GC Tsp45I 322 GTCAC ^GT[GC]AC TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 50 AGATCT [GA]^GATC[CT] XmaCI 150 CCCGGG C^CCGGG XmaI 150 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BanI 76 GGCACC G^G[CT][GA]CC 235 GGCGCC BsgI 145 CTGCAC GTGCAG(16/14) 259 CTGCAC BsiEI 356 CGGCCG CG[GA][CT]^CG 392 CGGTCG BsiYI 144 CCTGCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 273 CCTGCGCGTGG BslI 144 CCTGCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 273 CCTGCGCGTGG BsmAI 82 GAGAC GTCTC(1/5) 3 GTCTC BsmFI 377 GGGAC GGGAC(10/14) 306 GTCCC BstNI 327 CCTGG CC^[AT]GG 374 CCTGG EarI 97 CTCTTC CTCTTC(1/4) 213 GAAGAG EcoRII 327 CCTGG ^CC[AT]GG 374 CCTGG HapII 151 CCGG C^CGG 286 CCGG HpaII 151 CCGG C^CGG 286 CCGG HphI 210 GGTGA GGTGA(8/7) 323 TCACC Ksp632I 97 CTCTTC CTCTTC(1/4) 213 GAAGAG MboII 213 GAAGA GAAGA(8/7) 98 TCTTC MspI 151 CCGG C^CGG 286 CCGG MvaI 327 CCTGG CC^[AT]GG 374 CCTGG SapI 213 GAAGAGC GCTCTTC(1/4) 96 GCTCTTC TaqI 140 TCGA T^CGA 221 TCGA TthHB8I 140 TCGA T^CGA 221 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AccII AciI AluI AvaII BcnI BsaJI BssHII BssKI BstUI Cac8I CfoI Cfr13I DdeI EaeI FauI Fnu4HI HaeIII HhaI HinP1I ItaI MnlI MvnI MwoI NciI NlaIV Sau96I ScrFI