################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 737 GAGCTC G[AT]GC[AT]^C BalI 44 TGGCCA TGG^CCA BbrPI 166 CACGTG CAC^GTG BlpI 567 GCTGAGC GC^T[ACGT]AGC Bpu1102I 567 GCTGAGC GC^T[ACGT]AGC BseRI 145 GAGGAG GAGGAG(10/8) BsiHKAI 737 GAGCTC G[AT]GC[AT]^C BsmAI 958 GAGAC GTCTC(1/5) Bsp1407I 803 TGTACA T^GTACA BsrGI 803 TGTACA T^GTACA BstEII 432 GGTGACC G^GT[ACGT]ACC BstPI 432 GGTGACC G^GT[ACGT]ACC CelII 567 GCTGAGC GC^T[ACGT]AGC DpnII 978 GATC ^GATC EarI 358 CTCTTC CTCTTC(1/4) EcoO65I 432 GGTGACC G^GT[ACGT]ACC HphI 432 GGTGA GGTGA(8/7) Ksp632I 358 CTCTTC CTCTTC(1/4) KspI 219 CCGCGG CCGC^GG MboI 978 GATC ^GATC MboII 359 TCTTC GAAGA(8/7) MluNI 44 TGGCCA TGG^CCA MscI 44 TGGCCA TGG^CCA NdeII 978 GATC ^GATC PmaCI 166 CACGTG CAC^GTG PmlI 166 CACGTG CAC^GTG SacI 737 GAGCTC GAGCT^C SacII 219 CCGCGG CCGC^GG SapI 357 GCTCTTC GCTCTTC(1/4) Sau3AI 978 GATC ^GATC SbfI 813 CCTGCAGG CCTGCA^GG Sse8387I 813 CCTGCAGG CCTGCA^GG SspBI 803 TGTACA T^GTACA StuI 527 AGGCCT AGG^CCT TspRI 943 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 180 GTAC GT^AC 804 GTAC BbeI 640 GGCGCC GGCGC^C 675 GGCGCC BsaAI 166 CACGTG [CT]AC^GT[GA] 181 TACGTG BsrBI 213 CCGCTC CCGCTC(-3/-3) 516 GAGCGG BsrI 839 ACTGG ACTGG(1/-1) 177 CCAGT BssSI 511 CACGAG CACGAG(-5/-1) 1075 CTCGTG DdeI 568 CTGAG C^T[ACGT]AG 740 CTCAG DsaI 204 CCACGG C^C[GA][CT]GG 219 CCGCGG HgaI 452 GCGTC GACGC(5/10) 634 GCGTC HinfI 490 GACTC G^A[ACGT]TC 964 GACTC KasI 640 GGCGCC G^GCGCC 675 GGCGCC MslI 162 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1035 CATGAGGGTG NarI 640 GGCGCC GG^CGCC 675 GGCGCC PflMI 177 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 645 CCACAAGCTGG PleI 490 GACTC GAGTC(4/5) 964 GACTC RsaI 180 GTAC GT^AC 804 GTAC SfoI 640 GGCGCC GGC^GCC 675 GGCGCC Van91I 177 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 645 CCACAAGCTGG XcmI 326 CCAACAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1007 CCAGGCCCCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PstI PvuII Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI Tsp45I TthHB8I XmaCI XmaI