(Circular) (Six-base) MAPSORT of: pFN21AB5992.seq Check: 9767 from: 1 to: 6532 pFN21AB5992 6532 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4342 278 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 4659 5032 1563 Size: 3096 373 3063 Fragments arranged by size: 3096 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2238 2994 3018 3523 3804 4595 5979 6247 Size: 606 756 24 505 281 791 1384 268 Cuts at: 6247 1632 Size: 1917 Fragments arranged by size: 1917 1384 791 756 606 505 281 268 24 AfeI AGC'GCT Cuts at: 3636 3636 Size: 6532 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 5431 6408 1203 Size: 4228 977 1327 Fragments arranged by size: 4228 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 2887 5261 2887 Size: 2374 4158 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2620 1184 Size: 1436 5096 AlwNI CAG_nnn'CTG Cuts at: 2435 4275 5847 2435 Size: 1840 1572 3120 Fragments arranged by size: 3120 1840 1572 ApaLI G'TGCA_C Cuts at: 4589 5745 4589 Size: 1156 5376 ApoI r'AATT_y Cuts at: 1493 2044 2313 2486 2689 3841 4067 4219 6328 Size: 551 269 173 203 1152 226 152 2109 Cuts at: 6328 1493 Size: 1697 Fragments arranged by size: 2109 1697 1152 551 269 226 203 173 152 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3442 3475 3442 Size: 33 6499 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3853 5308 618 Size: 325 217 627 2066 1455 1842 Fragments arranged by size: 2066 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3851 1391 Size: 2460 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2371 2469 3091 928 Size: 971 345 127 98 622 4369 Fragments arranged by size: 4369 971 622 345 127 98 BbvCI CC'TCA_GC Cuts at: 3093 3093 Size: 6532 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4723 4757 1903 Size: 34 2786 34 3678 Fragments arranged by size: 3678 2786 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4424 5634 3125 Size: 1299 1210 4023 Fragments arranged by size: 4023 1299 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BfrBI ATG'CAT Cuts at: 2213 2672 2213 Size: 459 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5143 136 Size: 107 122 71 631 605 3471 1525 Fragments arranged by size: 3471 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6527 6527 Size: 6532 BlpI GC'TnA_GC Cuts at: 3949 3949 Size: 6532 Bme1580I G_kGCm'C Cuts at: 1790 4593 5749 1790 Size: 2803 1156 2573 Fragments arranged by size: 2803 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 4263 5221 Size: 416 53 83 399 24 738 2108 958 Cuts at: 5221 442 Size: 1753 Fragments arranged by size: 2108 1753 958 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2259 2385 3462 5192 1239 Size: 1020 126 1077 1730 2579 Fragments arranged by size: 2579 1730 1077 1020 126 Bpu10I CC'TnA_GC Cuts at: 3093 3624 3093 Size: 531 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3728 4657 5522 5820 3728 Size: 929 865 298 4440 Fragments arranged by size: 4440 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5195 882 Size: 617 3696 2219 Fragments arranged by size: 3696 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 3350 6526 3350 Size: 3176 3356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4339 4721 5356 275 Size: 53 83 186 936 2806 382 635 1451 Fragments arranged by size: 2806 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2832 1186 Size: 1646 4886 BseYI C'CCAG_C Cuts at: 3324 5735 6231 3324 Size: 2411 496 3625 Fragments arranged by size: 3625 2411 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2697 3490 1163 Size: 573 961 793 4205 Fragments arranged by size: 4205 961 793 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2808 4593 4678 5749 2808 Size: 1785 85 1071 3591 Fragments arranged by size: 3591 1785 1071 85 BsmI GAATG_Cn' Cuts at: 2609 2912 3185 3578 4043 4136 2609 Size: 303 273 393 465 93 5005 Fragments arranged by size: 5005 465 393 303 273 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2808 3851 4593 4678 5749 1391 Size: 399 1018 1043 742 85 1071 2174 Fragments arranged by size: 2174 1071 1043 1018 742 399 85 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 2478 3070 4421 6151 2478 Size: 592 1351 1730 2859 Fragments arranged by size: 2859 1730 1351 592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 3887 844 Size: 1443 1600 3489 Fragments arranged by size: 3489 1600 1443 BsrBI CCG'CTC Cuts at: 1394 1823 3431 4419 1394 Size: 429 1608 988 3507 Fragments arranged by size: 3507 1608 988 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3717 5027 5201 65 Size: 1183 2469 1310 174 1396 Fragments arranged by size: 2469 1396 1310 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5176 5363 1700 Size: 110 3366 187 2869 Fragments arranged by size: 3366 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2797 96 Size: 2701 3831 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 3813 4592 5604 3813 Size: 779 1012 4741 Fragments arranged by size: 4741 1012 779 BstAPI GCAn_nnn'nTGC Cuts at: 794 2219 794 Size: 1425 5107 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstXI CCAn_nnnn'nTGG Cuts at: 3364 3364 Size: 6532 BstYI r'GATC_y Cuts at: 1972 3610 3862 4229 4614 4631 6083 6169 6181 Size: 1638 252 367 385 17 1452 86 12 Cuts at: 6181 6527 1972 Size: 346 1977 Fragments arranged by size: 1977 1638 1452 385 367 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5980 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 2063 4048 4842 4862 2063 Size: 1985 794 20 3733 Fragments arranged by size: 3733 1985 794 20 ClaI AT'CG_AT Cuts at: 1554 2408 1554 Size: 854 5678 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 3836 3836 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3348 3496 3979 Size: 54 1425 50 275 18 1518 148 483 Cuts at: 3979 4868 6497 8 Size: 889 1629 43 Fragments arranged by size: 1629 1518 1425 889 483 275 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3263 3780 4462 1353 Size: 999 911 517 682 3423 Fragments arranged by size: 3423 999 911 682 517 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5119 5493 5639 387 Size: 1064 138 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2259 2385 2994 3018 3462 3523 Size: 393 606 21 126 609 24 444 61 Cuts at: 3523 3804 4595 5192 5979 6247 1239 Size: 281 791 597 787 268 1524 Fragments arranged by size: 1524 791 787 609 606 597 444 393 281 268 126 61 24 21 EcoO109I rG'GnC_Cy Cuts at: 2697 2697 Size: 6532 EcoRI G'AATT_C Cuts at: 3841 4219 3841 Size: 378 6154 FspI TGC'GCA Cuts at: 5038 5038 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 3638 5679 1678 Size: 1960 2041 2531 Fragments arranged by size: 2531 2041 1960 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2873 2905 5173 Size: 32 135 32 662 32 607 32 2268 Cuts at: 5173 5205 5247 5279 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2268 662 607 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3876 4122 669 Size: 988 281 1938 246 3079 Fragments arranged by size: 3079 1938 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6532 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 2223 4131 2223 Size: 1908 4624 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3318 3720 5646 5830 1305 Size: 2013 402 1926 184 2007 Fragments arranged by size: 2013 2007 1926 402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3350 3498 10 Size: 54 1425 343 1518 148 3044 Fragments arranged by size: 3044 1518 1425 343 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2673 2799 4490 4849 5008 518 Size: 648 907 600 126 1691 359 159 2042 Fragments arranged by size: 2042 1691 907 648 600 359 159 126 MspA1I CmG'CkG Cuts at: 1606 3328 3948 4625 5773 6018 1606 Size: 1722 620 677 1148 245 2120 Fragments arranged by size: 2120 1722 1148 677 620 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5980 NdeI CA'TA_TG Cuts at: 387 2455 2867 3517 3565 387 Size: 2068 412 650 48 3354 Fragments arranged by size: 3354 2068 650 412 48 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NsiI A_TGCA'T Cuts at: 2215 2674 2215 Size: 459 6073 NspI r_CATG'y Cuts at: 1132 1229 2672 2676 3890 5435 1132 Size: 97 1443 4 1214 1545 2229 Fragments arranged by size: 2229 1545 1443 1214 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3235 1108 Size: 902 1225 4405 Fragments arranged by size: 4405 1225 902 PfoI T'CCnGG_A Cuts at: 2781 3219 2781 Size: 438 6094 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2697 2697 Size: 6532 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PstI C_TGCA'G Cuts at: 830 2122 2348 2841 3884 830 Size: 1292 226 493 1043 3478 Fragments arranged by size: 3478 1292 1043 493 226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 3328 3328 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SanDI GG'GwC_CC Cuts at: 2697 2697 Size: 6532 SbfI CC_TGCA'GG Cuts at: 3884 3884 Size: 6532 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SfcI C'TryA_G Cuts at: 826 1045 2118 2344 2837 3880 5015 5696 5887 Size: 219 1073 226 493 1043 1135 681 191 Cuts at: 5887 826 Size: 1471 Fragments arranged by size: 1471 1135 1073 1043 681 493 226 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3707 4636 5537 5799 820 Size: 197 926 1764 929 901 262 1553 Fragments arranged by size: 1764 1553 929 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 2776 152 Size: 2624 3908 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SspI AAT'ATT Cuts at: 5 52 3077 3278 4456 5 Size: 47 3025 201 1178 2081 Fragments arranged by size: 3025 2081 1178 201 47 StuI AGG'CCT Cuts at: 1517 3735 1517 Size: 2218 4314 StyI C'CwwG_G Cuts at: 513 1065 3754 3971 513 Size: 552 2689 217 3074 Fragments arranged by size: 3074 2689 552 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2002 4559 4576 4729 4914 5359 325 Size: 1255 422 2557 17 153 185 445 1498 Fragments arranged by size: 2557 1498 1255 445 422 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2114 2797 4778 Size: 274 80 33 51 1443 137 683 1981 Cuts at: 4778 6490 96 Size: 1712 138 Fragments arranged by size: 1981 1712 1443 683 274 138 137 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 3334 1316 Size: 2018 4514 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6532 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 2089 2486 3155 4661 2089 Size: 397 669 1506 3960 Fragments arranged by size: 3960 1506 669 397 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4340 276 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI