(Circular) (Six-base) MAPSORT of: pFN21AE0705.seq Check: 2865 from: 1 to: 5590 pFN21AE0705 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 2933 1937 Size: 72 924 4594 Fragments arranged by size: 4594 924 72 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 2694 3653 5037 5305 1632 Size: 716 346 959 1384 268 1917 Fragments arranged by size: 1917 1384 959 716 346 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2584 1166 Size: 1418 4172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 1184 Size: 948 4642 AlwNI CAG_nnn'CTG Cuts at: 2672 3333 4905 2672 Size: 661 1572 3357 Fragments arranged by size: 3357 1572 661 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 5590 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2308 2356 2521 2899 3125 3277 5386 1493 Size: 815 48 165 378 226 152 2109 1697 Fragments arranged by size: 2109 1697 815 378 226 165 152 48 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2754 2787 2754 Size: 33 5557 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2339 2909 1391 Size: 948 570 4072 Fragments arranged by size: 4072 948 570 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2339 3651 4807 1790 Size: 549 1312 1156 2573 Fragments arranged by size: 2573 1312 1156 549 BmgBI CAC'GTC Cuts at: 1318 2275 2384 1318 Size: 957 109 4524 Fragments arranged by size: 4524 957 109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3321 4279 Size: 416 53 83 399 24 639 1265 958 Cuts at: 4279 442 Size: 1753 Fragments arranged by size: 1753 1265 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 4250 1239 Size: 790 2221 2579 Fragments arranged by size: 2579 2221 790 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2805 3715 4580 4878 2805 Size: 910 865 298 3517 Fragments arranged by size: 3517 910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2661 2691 1178 Size: 30 165 30 1258 30 4077 Fragments arranged by size: 4077 1258 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2397 1186 Size: 1211 4379 BseYI C'CCAG_C Cuts at: 2332 4793 5289 2332 Size: 2461 496 2633 Fragments arranged by size: 2633 2461 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 2396 1163 Size: 573 567 93 4357 Fragments arranged by size: 4357 573 567 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3651 3736 4807 3651 Size: 85 1071 4434 Fragments arranged by size: 4434 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2909 3651 3736 4807 1391 Size: 399 549 570 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 549 399 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 2701 3479 5209 2701 Size: 778 1730 3082 Fragments arranged by size: 3082 1730 778 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2880 4085 4259 65 Size: 1183 1632 1205 174 1396 Fragments arranged by size: 1632 1396 1205 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstXI CCAn_nnnn'nTGG Cuts at: 2586 2586 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2314 513 Size: 552 1249 3789 Fragments arranged by size: 3789 1249 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2370 2405 529 Size: 35 1806 35 3714 Fragments arranged by size: 3714 1806 35 35 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555 Size: 54 1425 50 275 18 1207 889 1629 Cuts at: 5555 8 Size: 43 Fragments arranged by size: 1629 1425 1207 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3520 1353 Size: 2167 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2348 2694 3653 4250 5037 5305 Size: 393 397 319 346 959 597 787 268 Cuts at: 5305 1239 Size: 1524 Fragments arranged by size: 1524 959 787 597 397 393 346 319 268 EcoO109I rG'GnC_Cy Cuts at: 2336 2336 Size: 5590 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 EcoRV GAT'ATC Cuts at: 2549 2549 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 4737 1678 Size: 3059 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2641 2934 3180 669 Size: 988 281 703 293 246 3079 Fragments arranged by size: 3079 988 703 293 281 246 HindIII A'AGCT_T Cuts at: 748 2176 748 Size: 1428 4162 HpaI GTT'AAC Cuts at: 2641 3180 2641 Size: 539 5051 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2116 4704 4888 1305 Size: 811 2588 184 2007 Fragments arranged by size: 2588 2007 811 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3768 Fragments arranged by size: 3768 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2584 2652 3548 3907 4066 518 Size: 648 1418 68 896 359 159 2042 Fragments arranged by size: 2042 1418 896 648 359 159 68 MspA1I CmG'CkG Cuts at: 1606 2075 2636 3006 3683 4831 5076 1606 Size: 469 561 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 561 469 370 245 NcoI C'CATG_G Cuts at: 513 1065 2314 513 Size: 552 1249 3789 Fragments arranged by size: 3789 1249 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2720 1108 Size: 1612 3978 PfoI T'CCnGG_A Cuts at: 2665 2665 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 5590 PstI C_TGCA'G Cuts at: 830 2942 830 Size: 2112 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2075 2636 2075 Size: 561 5029 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2938 4073 4754 4945 826 Size: 219 1893 1135 681 191 1471 Fragments arranged by size: 1893 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2820 3694 4595 4857 820 Size: 197 926 877 874 901 262 1553 Fragments arranged by size: 1553 926 901 877 874 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2086 2314 3029 513 Size: 552 1021 228 715 3074 Fragments arranged by size: 3074 1021 715 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2766 3617 3634 3787 3972 4417 Size: 1255 495 691 851 17 153 185 445 Cuts at: 4417 325 Size: 1498 Fragments arranged by size: 1498 1255 851 691 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2464 2496 5550 5582 2464 Size: 32 3054 32 2472 Fragments arranged by size: 3054 2472 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI