Restriction map for
pFN21AB5745
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AhdI AocI AscI
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiWI BsmBI
BsoBI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII CpoI DraI
DraIII Eam1105I Eco81I EcoRV FseI
FspI HgaI Hin1I HindIII KasI
KspI MluI MroI MvnI NarI
NheI NotI NruI NspV PaeR7I
PflFI PmaCI PmeI PmlI Psp1406I
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1321 GGTACC G^GTACC
AgeI 828 ACCGGT A^CCGGT
Alw44I 2161 GTGCAC G^TGCAC
Aor51HI 1412 AGCGCT AGC^GCT
ApaI 1794 GGGCCC GGGCC^C
ApaLI 2161 GTGCAC G^TGCAC
AseI 99 ATTAAT AT^TAAT
AsnI 99 ATTAAT AT^TAAT
Asp718I 1321 GGTACC G^GTACC
BamHI 1400 GGATCC G^GATCC
BcgI 1223 CGATCGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2100 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1994 GAGACC GGTCTC(1/5)
BsmI 2353 GCATTC GAATGC(1/-1)
Bsp1407I 488 TGTACA T^GTACA
BspDI 779 ATCGAT AT^CGAT
BspLU11I 491 ACATGT A^CATGT
BsrDI 903 CATTGC GCAATG(2/0)
BsrGI 488 TGTACA T^GTACA
ClaI 779 ATCGAT AT^CGAT
DrdI 1996 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 555 CGGCCG C^GGCCG
EclXI 555 CGGCCG C^GGCCG
Eco47III 1412 AGCGCT AGC^GCT
Eco52I 555 CGGCCG C^GGCCG
FauI 1713 GCGGG CCCGC(4/6)
HincII 1590 GTTAAC GT[CT]^[GA]AC
HindII 1590 GTTAAC GT[CT]^[GA]AC
HpaI 1590 GTTAAC GTT^AAC
KpnI 1321 GGTACC GGTAC^C
MamI 2100 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1811 CAATTG C^AATTG
MspA1I 1191 CAGCTG C[AC]G^C[GT]G
MunI 1811 CAATTG C^AATTG
NaeI 2030 GCCGGC GCC^GGC
NcoI 2055 CCATGG C^CATGG
NdeI 1679 CATATG CA^TATG
NgoMIV 2030 GCCGGC G^CCGGC
PacI 100 TTAATTAA TTAAT^TAA
PinAI 828 ACCGGT A^CCGGT
PshAI 1925 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 99 ATTAAT AT^TAAT
PvuI 1223 CGATCG CGAT^CG
PvuII 1191 CAGCTG CAG^CTG
SapI 690 GCTCTTC GCTCTTC(1/4)
SphI 1229 GCATGC GCATG^C
SspBI 488 TGTACA T^GTACA
SspI 1063 AATATT AAT^ATT
StuI 1256 AGGCCT AGG^CCT
TaqI 780 TCGA T^CGA
TthHB8I 780 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 491 ACATGT A^C[GA][CT]GT
1379 ACGTGT
AspHI 2161 GTGCAC G[AT]GC[AT]^C
2208 GAGCAC
BanII 574 GAGCCC G[GA]GC[CT]^C
1794 GGGCCC
BbsI 1447 GTCTTC GAAGAC(2/6)
2251 GTCTTC
BclI 1697 TGATCA T^GATCA
2099 TGATCA
BcnI 1404 CCGGG CC^[GC]GG
2174 CCGGG
BpuAI 1447 GTCTTC GAAGAC(2/6)
2251 GTCTTC
BsaWI 665 ACCGGA [AT]^CCGG[AT]
828 ACCGGT
BsiEI 555 CGGCCG CG[GA][CT]^CG
1223 CGATCG
BsiHKAI 2161 GTGCAC G[AT]GC[AT]^C
2208 GAGCAC
BsmAI 418 GAGAC GTCTC(1/5)
1994 GAGAC
BstXI 977 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2049 CCACTTCCATGG
DsaI 638 CCGTGG C^C[GA][CT]GG
2055 CCATGG
EcoNI 79 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1242 CCTCAAGAAGG
EcoT22I 1357 ATGCAT ATGCA^T
2351 ATGCAT
FbaI 1697 TGATCA T^GATCA
2099 TGATCA
MaeII 316 ACGT A^CGT
1379 ACGT
NciI 1404 CCGGG CC^[GC]GG
2174 CCGGG
NsiI 1357 ATGCAT ATGCA^T
2351 ATGCAT
PpuMI 213 GGGACCC [GA]G^G[AT]CC[CT]
2191 GGGACCT
ScaI 1028 AGTACT AGT^ACT
1907 AGTACT
SmlI 1234 CTCAAG C^T[CT][GA]AG
1243 CTCAAG
TfiI 4 GATTC G^A[AT]TC
847 GATTC
XbaI 811 TCTAGA T^CTAGA
1512 TCTAGA
XcmI 307 CCAAATGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
974 CCACCATCGTCATGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BalI
BanI BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI