Restriction map for
pFN21AB5745
(Circular) (Six-base) MAPSORT of: pFN21AB5745.seq Check: 2834 from: 1 to: 7084
pFN21AB5745 7084 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3127 3299 3537 3127
Size: 172 238 6674
Fragments arranged by size:
6674 238 172
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4894 278
Size: 53 83 186 936 3358 2468
Fragments arranged by size:
3358 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4427 1937
Size: 2490 4594
Acc65I G'GTAC_C
Cuts at: 3326 4405 3326
Size: 1079 6005
AclI AA'CG_TT
Cuts at: 1563 5211 5584 1563
Size: 3648 373 3063
Fragments arranged by size:
3648 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2400 2683 3023 3983 4092 5147 6531
Size: 600 168 283 340 960 109 1055 1384
Cuts at: 6531 6799 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1055 960 600 340 283 268
168 109
AfeI AGC'GCT
Cuts at: 3419 3419
Size: 7084
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6887
AflIII A'CryG_T
Cuts at: 1203 2496 3384 5983 6960 1203
Size: 1293 888 2599 977 1327
Fragments arranged by size:
2599 1327 1293 977 888
AgeI A'CCGG_T
Cuts at: 2833 5915 2833
Size: 3082 4002
AhdI GACnn_n'nnGTC
Cuts at: 5813 5813
Size: 7084
AleI CACnn'nnGTG
Cuts at: 1166 3303 1166
Size: 2137 4947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3436 1184
Size: 2252 4832
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2311 2343 2000
Size: 32 279 32 6741
Fragments arranged by size:
6741 279 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2255 2855 4309 4827 6399 2255
Size: 600 1454 518 1572 2940
Fragments arranged by size:
2940 1572 1454 600 518
ApaI G_GGCC'C
Cuts at: 3803 3803
Size: 7084
ApaLI G'TGCA_C
Cuts at: 4166 5141 6297 4166
Size: 975 1156 4953
Fragments arranged by size:
4953 1156 975
ApoI r'AATT_y
Cuts at: 1493 2374 2708 2903 3537 3689 4393 4619 4771
Size: 881 334 195 634 152 704 226 152
Cuts at: 4771 6880 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 881 704 634 334 226 195
152 152
AseI AT'TA_AT
Cuts at: 160 1032 2105 5638 160
Size: 872 1073 3533 1606
Fragments arranged by size:
3533 1606 1073 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7084
AvaI C'yCGr_G
Cuts at: 1870 1943 4409 1870
Size: 73 2466 4545
Fragments arranged by size:
4545 2466 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3318 3351 3318
Size: 33 7051
BamHI G'GATC_C
Cuts at: 3405 4414 4781 3405
Size: 1009 367 5708
Fragments arranged by size:
5708 1009 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 3214 3326 4405 5860
Size: 325 217 627 401 1026 112 1079 1455
Cuts at: 5860 618
Size: 1842
Fragments arranged by size:
1842 1455 1079 1026 627 401 325 217
112
BanII G_rGCy'C
Cuts at: 1391 2583 3803 4403 1391
Size: 1192 1220 600 4072
Fragments arranged by size:
4072 1220 1192 600
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3445 4249 928
Size: 971 1546 804 3763
Fragments arranged by size:
3763 1546 971 804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3217 3251 5275 5309 1903
Size: 34 1280 34 2024 34 3678
Fragments arranged by size:
3678 2024 1280 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4976 6186 4976
Size: 1210 5874
BclI T'GATC_A
Cuts at: 1548 3702 4104 1548
Size: 2154 402 4528
Fragments arranged by size:
4528 2154 402
BfrBI ATG'CAT
Cuts at: 3364 4358 3364
Size: 994 6090
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5695 136
Size: 107 122 71 631 605 4023 1525
Fragments arranged by size:
4023 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7079 7079
Size: 7084
BlpI GC'TnA_GC
Cuts at: 4501 4501
Size: 7084
Bme1580I G_kGCm'C
Cuts at: 1790 3415 3803 3910 4170 4210 5145 6301 1790
Size: 1625 388 107 260 40 935 1156 2573
Fragments arranged by size:
2573 1625 1156 935 388 260 107 40
BmgBI CAC'GTC
Cuts at: 1318 3385 1318
Size: 2067 5017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3025 3904 4080
Size: 416 53 83 399 24 1608 879 176
Cuts at: 4080 4815 5773 442
Size: 735 958 1753
Fragments arranged by size:
1753 1608 958 879 735 416 399 176
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 3494 3902 4244 5744 1239
Size: 1143 1112 408 342 1500 2579
Fragments arranged by size:
2579 1500 1143 1112 408 342
Bpu10I CC'TnA_GC
Cuts at: 4211 4211
Size: 7084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 3233 5209 6074 6372 3224
Size: 9 1976 865 298 3936
Fragments arranged by size:
3936 1976 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3993 5747 882
Size: 617 2494 1754 2219
Fragments arranged by size:
2494 2219 1754 617
BsaAI yAC'GTr
Cuts at: 493 1204 4875 493
Size: 711 3671 2702
Fragments arranged by size:
3671 2702 711
BsaBI GATnn'nnATC
Cuts at: 4109 7078 4109
Size: 2969 4115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4891 5273 5908 275
Size: 53 83 186 936 3358 382 635 1451
Fragments arranged by size:
3358 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2670 2833 5517 5915 6189 6336 6747 2670
Size: 163 2684 398 274 147 411 3007
Fragments arranged by size:
3007 2684 411 398 274 163 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2395 2425 3008 3038 1178
Size: 30 165 30 992 30 583 30 5224
Fragments arranged by size:
5224 992 583 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3593 3596 3899 3986 1186
Size: 2407 3 303 87 4284
Fragments arranged by size:
4284 2407 303 87 3
BseYI C'CCAG_C
Cuts at: 2525 6287 6783 2525
Size: 3762 496 2826
Fragments arranged by size:
3762 2826 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2898 3327 4145 4361 1163
Size: 573 1162 429 818 216 3886
Fragments arranged by size:
3886 1162 818 573 429 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2563 3231 4534 5295 6323 1540
Size: 275 186 562 668 1303 761 1028 2301
Fragments arranged by size:
2301 1303 1028 761 668 562 275 186
BsiHKAI G_wGCw'C
Cuts at: 4170 4217 5145 5230 6301 4170
Size: 47 928 85 1071 4953
Fragments arranged by size:
4953 1071 928 85 47
BsmI GAATG_Cn'
Cuts at: 4358 4595 4688 4358
Size: 237 93 6754
Fragments arranged by size:
6754 237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 3415 3803 3910 4170 4210 4217
Size: 399 793 832 388 107 260 40 7
Cuts at: 4217 4403 5145 5230 6301 1391
Size: 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 832 793 742 399 388 260
186 107 85 40 7
BspEI T'CCGG_A
Cuts at: 6747 6747
Size: 7084
BspHI T'CATG_A
Cuts at: 4973 6703 4973
Size: 1730 5354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3127 3299 3537 4439 844
Size: 2283 172 238 902 3489
Fragments arranged by size:
3489 2283 902 238 172
BsrBI CCG'CTC
Cuts at: 1394 1823 4971 1394
Size: 429 3148 3507
Fragments arranged by size:
3507 3148 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2907 5579 5753 65
Size: 1183 1659 2672 174 1396
Fragments arranged by size:
2672 1659 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2833 3768 4035 5728 5915 1700
Size: 110 1023 935 267 1693 187 2869
Fragments arranged by size:
2869 1693 1023 935 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 2493 96
Size: 2397 4687
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7084
BssSI C'ACGA_G
Cuts at: 5144 6156 5144
Size: 1012 6072
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7084
BstBI TT'CG_AA
Cuts at: 5888 5888
Size: 7084
BstEII G'GTnAC_C
Cuts at: 1184 2885 2992 4009 1184
Size: 1701 107 1017 4259
Fragments arranged by size:
4259 1701 1017 107
BstXI CCAn_nnnn'nTGG
Cuts at: 2989 4061 2989
Size: 1072 6012
BstYI r'GATC_y
Cuts at: 1972 2606 3405 4041 4414 4781 5166 5183 6635
Size: 634 799 636 373 367 385 17 1452
Cuts at: 6635 6721 6733 7079 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 799 636 634 385 373 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2643 4060 513
Size: 552 939 639 1417 3537
Fragments arranged by size:
3537 1417 939 639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4099 487
Size: 683 56 2873 3472
Fragments arranged by size:
3472 2873 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 3298 3310 4168 4600 5394 5414 2044
Size: 1254 12 858 432 794 20 3714
Fragments arranged by size:
3714 1254 858 794 432 20 12
ClaI AT'CG_AT
Cuts at: 1554 2785 1554
Size: 1231 5853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7049
DraI TTT'AAA
Cuts at: 4388 4388
Size: 7084
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4007 6091 809
Size: 507 2691 2084 1802
Fragments arranged by size:
2691 2084 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2560 2601 3038
Size: 54 1425 50 275 18 730 41 437
Cuts at: 3038 3200 3917 4531 5420 7049 8
Size: 162 717 614 889 1629 43
Fragments arranged by size:
1629 1425 889 730 717 614 437 275
162 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2560 4531 1537
Size: 275 748 1971 4090
Fragments arranged by size:
4090 1971 748 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2652 2702 3720 3810 5014 1353
Size: 780 519 50 1018 90 1204 3423
Fragments arranged by size:
3423 1204 1018 780 519 90 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2968 5671 6045 6191 387
Size: 1064 138 1379 2703 374 146 1280
Fragments arranged by size:
2703 1379 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2382 2400 2683 3023 3494 3902
Size: 393 600 150 18 283 340 471 408
Cuts at: 3902 3983 4092 4244 5147 5744 6531 6799 1239
Size: 81 109 152 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 600 597 471 408 393
340 283 268 152 150 109 81 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2088 3251 2088
Size: 1163 5921
EcoO109I rG'GnC_Cy
Cuts at: 2219 3799 3800 4197 2219
Size: 1580 1 397 5106
Fragments arranged by size:
5106 1580 397 1
EcoRI G'AATT_C
Cuts at: 2374 2708 3689 4393 4771 2374
Size: 334 981 704 378 4687
Fragments arranged by size:
4687 981 704 378 334
FspI TGC'GCA
Cuts at: 5590 5590
Size: 7084
HaeII r_GCGC'y
Cuts at: 1678 3172 3421 4179 6231 1678
Size: 1494 249 758 2052 2531
Fragments arranged by size:
2531 2052 1494 758 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3836 3868 4346 4378 5725
Size: 32 135 32 2264 32 478 32 1347
Cuts at: 5725 5757 5799 5831 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2264 1347 478 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3597 4428 4674 669
Size: 988 281 1659 831 246 3079
Fragments arranged by size:
3079 1659 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7084
HpaI GTT'AAC
Cuts at: 3597 4674 3597
Size: 1077 6007
KpnI G_GTAC'C
Cuts at: 3330 4409 3330
Size: 1079 6005
MfeI C'AATT_G
Cuts at: 3816 4683 3816
Size: 867 6217
MluI A'CGCG_T
Cuts at: 6960 6960
Size: 7084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2183 2580 6198 6382 1305
Size: 878 397 3618 184 2007
Fragments arranged by size:
3618 2007 878 397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 3040 3202 3919 10
Size: 54 1425 343 771 437 162 717 3175
Fragments arranged by size:
3175 1425 771 717 437 343 162 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 3303 4059 4163 5042 5401 5560
Size: 648 1821 316 756 104 879 359 159
Cuts at: 5560 518
Size: 2042
Fragments arranged by size:
2042 1821 879 756 648 359 316 159
104
MspA1I CmG'CkG
Cuts at: 1606 3198 4500 5177 6325 6570 1606
Size: 1592 1302 677 1148 245 2120
Fragments arranged by size:
2120 1592 1302 1148 677 245
NaeI GCC'GGC
Cuts at: 4037 4037
Size: 7084
NcoI C'CATG_G
Cuts at: 513 1065 2004 4060 513
Size: 552 939 2056 3537
Fragments arranged by size:
3537 2056 939 552
NdeI CA'TA_TG
Cuts at: 387 3685 387
Size: 3298 3786
NgoMIV G'CCGG_C
Cuts at: 4035 4035
Size: 7084
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7084
NotI GC'GGCC_GC
Cuts at: 4531 4531
Size: 7084
NsiI A_TGCA'T
Cuts at: 3366 4360 3366
Size: 994 6090
NspI r_CATG'y
Cuts at: 1132 1229 2500 2730 3238 4442 5987 1132
Size: 97 1271 230 508 1204 1545 2229
Fragments arranged by size:
2229 1545 1271 1204 508 230 97
PacI TTA_AT'TAA
Cuts at: 2109 2109
Size: 7084
PasI CC'CwG_GG
Cuts at: 1348 2364 3554 1348
Size: 1016 1190 4878
Fragments arranged by size:
4878 1190 1016
PciI A'CATG_T
Cuts at: 2496 5983 2496
Size: 3487 3597
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3139 3209 3926 4309 1108
Size: 2031 70 717 383 3883
Fragments arranged by size:
3883 2031 717 383 70
PfoI T'CCnGG_A
Cuts at: 4221 4221
Size: 7084
PmeI GTTT'AAAC
Cuts at: 4388 4388
Size: 7084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5153 5185 6666 6698 5153
Size: 32 1481 32 5539
Fragments arranged by size:
5539 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2219 4197 2219
Size: 1978 5106
PshAI GACnn'nnGTC
Cuts at: 1613 3934 1613
Size: 2321 4763
PsiI TTA'TAA
Cuts at: 4654 4654
Size: 7084
PspOMI G'GGCC_C
Cuts at: 3799 3799
Size: 7084
PstI C_TGCA'G
Cuts at: 830 3305 3534 4175 4436 830
Size: 2475 229 641 261 3478
Fragments arranged by size:
3478 2475 641 261 229
PvuI CG_AT'CG
Cuts at: 2001 3231 2001
Size: 1230 5854
PvuII CAG'CTG
Cuts at: 3198 3198
Size: 7084
SalI G'TCGA_C
Cuts at: 1936 4426 1936
Size: 2490 4594
SanDI GG'GwC_CC
Cuts at: 2219 2219
Size: 7084
SapI GCTCTTCn'nnn_
Cuts at: 2702 2702
Size: 7084
SbfI CC_TGCA'GG
Cuts at: 4436 4436
Size: 7084
ScaI AGT'ACT
Cuts at: 3035 3914 5332 7044 3035
Size: 879 1418 1712 3075
Fragments arranged by size:
3075 1712 1418 879
SfcI C'TryA_G
Cuts at: 826 1045 2088 2292 3081 3301 3530 4171 4283
Size: 219 1043 204 789 220 229 641 112
Cuts at: 4283 4432 5567 6248 6439 826
Size: 149 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 789 681 641 229 220
219 204 191 149 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7084
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7084
SmaI CCC'GGG
Cuts at: 1872 4411 1872
Size: 2539 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3239 3248 5188 6089 6351 820
Size: 197 926 1296 9 1940 901 262 1553
Fragments arranged by size:
1940 1553 1296 926 901 262 197 9
SnaBI TAC'GTA
Cuts at: 493 4875 493
Size: 4382 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7084
SphI G_CATG'C
Cuts at: 1132 3238 4442 1132
Size: 2106 1204 3774
Fragments arranged by size:
3774 2106 1204
SspI AAT'ATT
Cuts at: 5 52 3070 5008 5
Size: 47 3018 1938 2081
Fragments arranged by size:
3018 2081 1938 47
StuI AGG'CCT
Cuts at: 1517 3263 1517
Size: 1746 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 3046 4060 4523 513
Size: 552 939 756 286 1014 463 3074
Fragments arranged by size:
3074 1014 939 756 552 463 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5111 5128 5281 5466 5911 325
Size: 1255 3531 17 153 185 445 1498
Fragments arranged by size:
3531 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 3033 3912
Size: 274 80 33 51 1443 516 540 879
Cuts at: 3912 5330 7042 96
Size: 1418 1712 138
Fragments arranged by size:
1712 1443 1418 879 540 516 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 4189 4221 4284 4316 7044 7076 3008
Size: 32 1149 32 63 32 2728 32 3016
Fragments arranged by size:
3016 2728 1149 63 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7084
XbaI T'CTAG_A
Cuts at: 2816 3517 4420 2816
Size: 701 903 5480
Fragments arranged by size:
5480 903 701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2319 2986 2319
Size: 667 6417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7084
XmaI C'CCGG_G
Cuts at: 1870 4409 1870
Size: 2539 4545
XmnI GAAnn'nnTTC
Cuts at: 2844 2903 2945 5213 2844
Size: 59 42 2268 4715
Fragments arranged by size:
4715 2268 59 42
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4892 276
Size: 53 83 186 936 3358 2468
Fragments arranged by size:
3358 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI PmlI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI