(Linear) (Six-base) MAPSORT of: KIBB5745.seq Check: 8843 from: 1 to: 2379 KIBB5745.orf With 182 enzymes: SgfI * July 3, 2012 16:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1122 1294 1532 2379 Size: 1122 172 238 847 Fragments arranged by size: 1122 847 238 172 Acc65I G'GTAC_C Cuts at: 0 1321 2379 Size: 1321 1058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 395 678 1018 1978 2087 2379 Size: 227 168 283 340 960 109 292 Fragments arranged by size: 960 340 292 283 227 168 109 AfeI AGC'GCT Cuts at: 0 1414 2379 Size: 1414 965 AflIII A'CryG_T Cuts at: 0 491 1379 2379 Size: 491 888 1000 Fragments arranged by size: 1000 888 491 AgeI A'CCGG_T Cuts at: 0 828 2379 Size: 828 1551 AleI CACnn'nnGTG Cuts at: 0 1298 2379 Size: 1298 1081 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1431 2379 Size: 1431 948 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 306 338 2379 Size: 27 279 32 2041 Fragments arranged by size: 2041 279 32 27 AlwNI CAG_nnn'CTG Cuts at: 0 250 850 2304 2379 Size: 250 600 1454 75 Fragments arranged by size: 1454 600 250 75 ApaI G_GGCC'C Cuts at: 0 1798 2379 Size: 1798 581 ApaLI G'TGCA_C Cuts at: 0 2161 2379 Size: 2161 218 ApoI r'AATT_y Cuts at: 0 369 703 898 1532 1684 2379 Size: 369 334 195 634 152 695 Fragments arranged by size: 695 634 369 334 195 152 AseI AT'TA_AT Cuts at: 0 100 2379 Size: 100 2279 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1313 1346 2379 Size: 1313 33 1033 Fragments arranged by size: 1313 1033 33 BamHI G'GATC_C Cuts at: 0 1400 2379 Size: 1400 979 BanI G'GyrC_C Cuts at: 0 183 1209 1321 2379 Size: 183 1026 112 1058 Fragments arranged by size: 1058 1026 183 112 BanII G_rGCy'C Cuts at: 0 578 1798 2379 Size: 578 1220 581 Fragments arranged by size: 1220 581 578 BbsI GAAGACnn'nnnn_ Cuts at: 0 1440 2244 2379 Size: 1440 804 135 Fragments arranged by size: 1440 804 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1212 1246 2379 Size: 1212 34 1133 Fragments arranged by size: 1212 1133 34 BclI T'GATC_A Cuts at: 0 1697 2099 2379 Size: 1697 402 280 Fragments arranged by size: 1697 402 280 BfrBI ATG'CAT Cuts at: 0 1359 2353 2379 Size: 1359 994 26 Fragments arranged by size: 1359 994 26 Bme1580I G_kGCm'C Cuts at: 0 1410 1798 1905 2165 2205 2379 Size: 1410 388 107 260 40 174 Fragments arranged by size: 1410 388 260 174 107 40 BmgBI CAC'GTC Cuts at: 0 1380 2379 Size: 1380 999 BmrI ACTGGGnnnn_n' Cuts at: 0 1020 1899 2075 2379 Size: 1020 879 176 304 Fragments arranged by size: 1020 879 304 176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 1489 1897 2239 2379 Size: 377 1112 408 342 140 Fragments arranged by size: 1112 408 377 342 140 Bpu10I CC'TnA_GC Cuts at: 0 2206 2379 Size: 2206 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1219 1228 2379 Size: 1219 9 1151 Fragments arranged by size: 1219 1151 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 1988 2379 Size: 1988 391 BsaBI GATnn'nnATC Cuts at: 0 2104 2379 Size: 2104 275 BsaWI w'CCGG_w Cuts at: 0 665 828 2379 Size: 665 163 1551 Fragments arranged by size: 1551 665 163 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 390 420 1003 1033 2379 Size: 390 30 583 30 1346 Fragments arranged by size: 1346 583 390 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1588 1591 1894 1981 2379 Size: 1588 3 303 87 398 Fragments arranged by size: 1588 398 303 87 3 BseYI C'CCAG_C Cuts at: 0 520 2379 Size: 520 1859 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1322 2140 2356 2379 Size: 893 429 818 216 23 Fragments arranged by size: 893 818 429 216 23 BsiEI CG_ry'CG Cuts at: 0 558 1226 2379 Size: 558 668 1153 Fragments arranged by size: 1153 668 558 BsiHKAI G_wGCw'C Cuts at: 0 2165 2212 2379 Size: 2165 47 167 Fragments arranged by size: 2165 167 47 BsmI GAATG_Cn' Cuts at: 0 2353 2379 Size: 2353 26 Bsp1286I G_dGCh'C Cuts at: 0 578 1410 1798 1905 2165 2205 2212 2379 Size: 578 832 388 107 260 40 7 167 Fragments arranged by size: 832 578 388 260 167 107 40 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1122 1294 1532 2379 Size: 1122 172 238 847 Fragments arranged by size: 1122 847 238 172 BsrDI GCAATG_nn' Cuts at: 0 902 2379 Size: 902 1477 BsrFI r'CCGG_y Cuts at: 0 828 1763 2030 2379 Size: 828 935 267 349 Fragments arranged by size: 935 828 349 267 BsrGI T'GTAC_A Cuts at: 0 488 2379 Size: 488 1891 BstEII G'GTnAC_C Cuts at: 0 880 987 2004 2379 Size: 880 107 1017 375 Fragments arranged by size: 1017 880 375 107 BstXI CCAn_nnnn'nTGG Cuts at: 0 984 2056 2379 Size: 984 1072 323 Fragments arranged by size: 1072 984 323 BstYI r'GATC_y Cuts at: 0 601 1400 2036 2379 Size: 601 799 636 343 Fragments arranged by size: 799 636 601 343 BtgI C'CryG_G Cuts at: 0 638 2055 2379 Size: 638 1417 324 Fragments arranged by size: 1417 638 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2094 2379 Size: 2094 285 BtsI GCAGTG_nn' Cuts at: 0 39 1293 1305 2163 2379 Size: 39 1254 12 858 216 Fragments arranged by size: 1254 858 216 39 12 ClaI AT'CG_AT Cuts at: 0 780 2379 Size: 780 1599 DrdI GACnn_nn'nnGTC Cuts at: 0 2002 2379 Size: 2002 377 EaeI y'GGCC_r Cuts at: 0 555 596 1033 1195 1912 2379 Size: 555 41 437 162 717 467 Fragments arranged by size: 717 555 467 437 162 41 EagI C'GGCC_G Cuts at: 0 555 2379 Size: 555 1824 EarI CTCTTCn'nnn_ Cuts at: 0 128 647 697 1715 1805 2379 Size: 128 519 50 1018 90 574 Fragments arranged by size: 1018 574 519 128 90 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 963 2379 Size: 963 1416 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 377 395 678 1018 1489 1897 1978 Size: 227 150 18 283 340 471 408 81 Cuts at: 1978 2087 2239 2379 Size: 109 152 140 Fragments arranged by size: 471 408 340 283 227 152 150 140 109 81 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 83 1246 2379 Size: 83 1163 1133 Fragments arranged by size: 1163 1133 83 EcoO109I rG'GnC_Cy Cuts at: 0 214 1794 1795 2192 2379 Size: 214 1580 1 397 187 Fragments arranged by size: 1580 397 214 187 1 EcoRI G'AATT_C Cuts at: 0 369 703 1684 2379 Size: 369 334 981 695 Fragments arranged by size: 981 695 369 334 HaeII r_GCGC'y Cuts at: 0 1167 1416 2174 2379 Size: 1167 249 758 205 Fragments arranged by size: 1167 758 249 205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1831 1863 2341 2373 2379 Size: 1831 32 478 32 6 Fragments arranged by size: 1831 478 32 32 6 HincII GTy'rAC Cuts at: 0 1592 2379 Size: 1592 787 HpaI GTT'AAC Cuts at: 0 1592 2379 Size: 1592 787 KpnI G_GTAC'C Cuts at: 0 1325 2379 Size: 1325 1054 MfeI C'AATT_G Cuts at: 0 1811 2379 Size: 1811 568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 178 575 2379 Size: 178 397 1804 Fragments arranged by size: 1804 397 178 MscI TGG'CCA Cuts at: 0 598 1035 1197 1914 2379 Size: 598 437 162 717 465 Fragments arranged by size: 717 598 465 437 162 MslI CAynn'nnrTG Cuts at: 0 982 1298 2054 2158 2379 Size: 982 316 756 104 221 Fragments arranged by size: 982 756 316 221 104 MspA1I CmG'CkG Cuts at: 0 1193 2379 Size: 1193 1186 NaeI GCC'GGC Cuts at: 0 2032 2379 Size: 2032 347 NcoI C'CATG_G Cuts at: 0 2055 2379 Size: 2055 324 NdeI CA'TA_TG Cuts at: 0 1680 2379 Size: 1680 699 NgoMIV G'CCGG_C Cuts at: 0 2030 2379 Size: 2030 349 NsiI A_TGCA'T Cuts at: 0 1361 2355 2379 Size: 1361 994 24 Fragments arranged by size: 1361 994 24 NspI r_CATG'y Cuts at: 0 495 725 1233 2379 Size: 495 230 508 1146 Fragments arranged by size: 1146 508 495 230 PacI TTA_AT'TAA Cuts at: 0 104 2379 Size: 104 2275 PasI CC'CwG_GG Cuts at: 0 359 1549 2379 Size: 359 1190 830 Fragments arranged by size: 1190 830 359 PciI A'CATG_T Cuts at: 0 491 2379 Size: 491 1888 PflMI CCAn_nnn'nTGG Cuts at: 0 1134 1204 1921 2304 2379 Size: 1134 70 717 383 75 Fragments arranged by size: 1134 717 383 75 70 PfoI T'CCnGG_A Cuts at: 0 2216 2379 Size: 2216 163 PpuMI rG'GwC_Cy Cuts at: 0 214 2192 2379 Size: 214 1978 187 Fragments arranged by size: 1978 214 187 PshAI GACnn'nnGTC Cuts at: 0 1929 2379 Size: 1929 450 PspOMI G'GGCC_C Cuts at: 0 1794 2379 Size: 1794 585 PstI C_TGCA'G Cuts at: 0 1300 1529 2170 2379 Size: 1300 229 641 209 Fragments arranged by size: 1300 641 229 209 PvuI CG_AT'CG Cuts at: 0 1226 2379 Size: 1226 1153 PvuII CAG'CTG Cuts at: 0 1193 2379 Size: 1193 1186 SanDI GG'GwC_CC Cuts at: 0 214 2379 Size: 214 2165 SapI GCTCTTCn'nnn_ Cuts at: 0 697 2379 Size: 697 1682 ScaI AGT'ACT Cuts at: 0 1030 1909 2379 Size: 1030 879 470 Fragments arranged by size: 1030 879 470 SfcI C'TryA_G Cuts at: 0 83 287 1076 1296 1525 2166 2278 2379 Size: 83 204 789 220 229 641 112 101 Fragments arranged by size: 789 641 229 220 204 112 101 83 SmlI C'TyrA_G Cuts at: 0 1234 1243 2379 Size: 1234 9 1136 Fragments arranged by size: 1234 1136 9 SphI G_CATG'C Cuts at: 0 1233 2379 Size: 1233 1146 SspI AAT'ATT Cuts at: 0 1065 2379 Size: 1065 1314 StuI AGG'CCT Cuts at: 0 1258 2379 Size: 1258 1121 StyI C'CwwG_G Cuts at: 0 755 1041 2055 2379 Size: 755 286 1014 324 Fragments arranged by size: 1014 755 324 286 TatI w'GTAC_w Cuts at: 0 488 1028 1907 2379 Size: 488 540 879 472 Fragments arranged by size: 879 540 488 472 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1003 1035 2184 2216 2279 2311 2379 Size: 1003 32 1149 32 63 32 68 Fragments arranged by size: 1149 1003 68 63 32 32 32 XbaI T'CTAG_A Cuts at: 0 811 1512 2379 Size: 811 701 867 Fragments arranged by size: 867 811 701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 314 981 2379 Size: 314 667 1398 Fragments arranged by size: 1398 667 314 XmnI GAAnn'nnTTC Cuts at: 0 839 898 940 2379 Size: 839 59 42 1439 Fragments arranged by size: 1439 839 59 42 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI BaeI BamHI BanI BanII BbsI BcgI BclI BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PacI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuI PvuII SanDI SapI ScaI SfcI SmlI SphI SspI StuI StyI TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AflII AhdI AscI AsiSI AvaI AvrII BbeI BbvCI BciVI BglI BglII BlpI BmtI BplI BsaAI BsaHI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I CspCI DraI DraIII EcoICRI EcoRV FalI FseI FspI FspAI HindIII KasI MluI NarI NheI NotI NruI PmeI PmlI PpiI PsiI PspXI PsrI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII Tth111I XhoI XmaI ZraI