(Circular) (Six-base) MAPSORT of: pFN21AE4531.seq Check: 9321 from: 1 to: 6487 pFN21AE4531 6487 bp With 182 enzymes: SgfI * March 28, 2012 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2211 3642 2211 Size: 1431 5056 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3830 1937 Size: 1893 4594 Acc65I G'GTAC_C Cuts at: 3501 3808 3501 Size: 307 6180 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2017 2509 4550 5934 6202 1632 Size: 385 492 2041 1384 268 1917 Fragments arranged by size: 2041 1917 1384 492 385 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6290 AflIII A'CryG_T Cuts at: 1203 5386 6363 1203 Size: 4183 977 1327 Fragments arranged by size: 4183 1327 977 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 2468 1166 Size: 1302 5185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6487 AlwNI CAG_nnn'CTG Cuts at: 2449 2977 4230 5802 2449 Size: 528 1253 1572 3134 Fragments arranged by size: 3134 1572 1253 528 ApaI G_GGCC'C Cuts at: 2187 2457 3405 2187 Size: 270 948 5269 Fragments arranged by size: 5269 948 270 ApaLI G'TGCA_C Cuts at: 4544 5700 4544 Size: 1156 5331 ApoI r'AATT_y Cuts at: 1493 3796 4022 4174 6283 1493 Size: 2303 226 152 2109 1697 Fragments arranged by size: 2303 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 2239 2268 2293 2418 3586 3812 1870 Size: 73 296 29 25 125 1168 226 4545 Fragments arranged by size: 4545 1168 296 226 125 73 29 25 BamHI G'GATC_C Cuts at: 3817 4184 3817 Size: 367 6120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2170 2347 2425 3501 3808 Size: 325 217 627 383 177 78 1076 307 Cuts at: 3808 5263 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1076 627 383 325 307 217 177 78 BanII G_rGCy'C Cuts at: 1391 2187 2457 2913 3405 3460 3593 3806 1391 Size: 796 270 456 492 55 133 213 4072 Fragments arranged by size: 4072 796 492 456 270 213 133 55 BbeI G_GCGC'C Cuts at: 2174 2351 2429 2174 Size: 177 78 6232 Fragments arranged by size: 6232 177 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3157 3183 928 Size: 971 1258 26 4232 Fragments arranged by size: 4232 1258 971 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4678 4712 1903 Size: 34 50 34 2657 34 3678 Fragments arranged by size: 3678 2657 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4379 5589 4379 Size: 1210 5277 BclI T'GATC_A Cuts at: 1548 1548 Size: 6487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2250 2391 3311 Size: 107 122 71 631 605 578 141 920 Cuts at: 3311 3400 5098 136 Size: 89 1698 1525 Fragments arranged by size: 1698 1525 920 631 605 578 141 122 107 89 71 BglII A'GATC_T Cuts at: 6482 6482 Size: 6487 BlpI GC'TnA_GC Cuts at: 3904 3904 Size: 6487 Bme1580I G_kGCm'C Cuts at: 1790 2187 2457 3405 4548 5704 1790 Size: 397 270 948 1143 1156 2573 Fragments arranged by size: 2573 1156 1143 948 397 270 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3109 4218 5176 Size: 416 53 83 399 24 1692 1109 958 Cuts at: 5176 442 Size: 1753 Fragments arranged by size: 1753 1692 1109 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2631 2697 2631 Size: 66 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2218 2642 3042 3497 3563 3746 5147 1239 Size: 979 424 400 455 66 183 1401 2579 Fragments arranged by size: 2579 1401 979 455 424 400 183 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3368 4612 5477 5775 3368 Size: 1244 865 298 4080 Fragments arranged by size: 4080 1244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 2775 5150 882 Size: 617 1021 255 2375 2219 Fragments arranged by size: 2375 2219 1021 617 255 BsaAI yAC'GTr Cuts at: 493 1204 2470 3247 4278 493 Size: 711 1266 777 1031 2702 Fragments arranged by size: 2702 1266 1031 777 711 BsaBI GATnn'nnATC Cuts at: 6481 6481 Size: 6487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2171 2348 2426 4294 Size: 53 83 186 936 638 177 78 1868 Cuts at: 4294 4676 5311 275 Size: 382 635 1451 Fragments arranged by size: 1868 1451 936 638 635 382 186 177 83 78 53 BsaWI w'CCGG_w Cuts at: 2719 2913 4920 5318 5592 5739 6150 2719 Size: 194 2007 398 274 147 411 3056 Fragments arranged by size: 3056 2007 411 398 274 194 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2088 2118 3575 3605 1178 Size: 30 165 30 685 30 1457 30 4060 Fragments arranged by size: 4060 1457 685 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3014 3045 1186 Size: 1828 31 4628 Fragments arranged by size: 4628 1828 31 BseYI C'CCAG_C Cuts at: 2312 2449 5690 6186 2312 Size: 137 3241 496 2613 Fragments arranged by size: 3241 2613 496 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3227 1163 Size: 573 1491 4423 Fragments arranged by size: 4423 1491 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2265 3937 4698 5726 1540 Size: 275 186 57 207 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 207 186 57 BsiHKAI G_wGCw'C Cuts at: 2913 3578 4548 4633 5704 2913 Size: 665 970 85 1071 3696 Fragments arranged by size: 3696 1071 970 665 85 BsmI GAATG_Cn' Cuts at: 2861 2973 3998 4091 2861 Size: 112 1025 93 5257 Fragments arranged by size: 5257 1025 112 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2457 2913 3405 3460 3578 3593 Size: 399 397 270 456 492 55 118 15 Cuts at: 3593 3806 4548 4633 5704 1391 Size: 213 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 456 399 397 270 213 118 85 55 15 BspEI T'CCGG_A Cuts at: 2719 6150 2719 Size: 3431 3056 BspHI T'CATG_A Cuts at: 4376 6106 4376 Size: 1730 4757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 2718 3642 3775 3842 844 Size: 1367 507 924 133 67 3489 Fragments arranged by size: 3489 1367 924 507 133 67 BsrBI CCG'CTC Cuts at: 1394 1823 2088 2275 2954 4374 1394 Size: 429 265 187 679 1420 3507 Fragments arranged by size: 3507 1420 679 429 265 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4982 5156 65 Size: 1183 3734 174 1396 Fragments arranged by size: 3734 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 3310 5131 5318 1700 Size: 110 575 925 1821 187 2869 Fragments arranged by size: 2869 1821 925 575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6487 BssHII G'CGCG_C Cuts at: 1925 2114 2116 2283 2307 1925 Size: 189 2 167 24 6105 Fragments arranged by size: 6105 189 167 24 2 BssSI C'ACGA_G Cuts at: 3641 4547 5559 3641 Size: 906 1012 4569 Fragments arranged by size: 4569 1012 906 BstAPI GCAn_nnn'nTGC Cuts at: 794 3637 794 Size: 2843 3644 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstXI CCAn_nnnn'nTGG Cuts at: 2641 3306 3475 2641 Size: 665 169 5653 Fragments arranged by size: 5653 665 169 BstYI r'GATC_y Cuts at: 1972 3291 3817 4184 4569 4586 6038 6124 6136 Size: 1319 526 367 385 17 1452 86 12 Cuts at: 6136 6482 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1319 526 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2248 2694 2987 3255 3426 513 Size: 552 1183 446 293 268 171 3574 Fragments arranged by size: 3574 1183 552 446 293 268 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5748 Fragments arranged by size: 5748 683 56 BtsI GCAGTG_nn' Cuts at: 2341 3331 4003 4797 4817 2341 Size: 990 672 794 20 4011 Fragments arranged by size: 4011 990 794 672 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6452 DraI TTT'AAA Cuts at: 3791 3791 Size: 6487 DraIII CAC_nnn'GTG Cuts at: 2215 2215 Size: 6487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3563 5494 809 Size: 507 2247 1931 1802 Fragments arranged by size: 2247 1931 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2319 2361 Size: 54 1425 50 275 18 432 57 42 Cuts at: 2361 2697 3308 3619 3934 4823 6452 8 Size: 336 611 311 315 889 1629 43 Fragments arranged by size: 1629 1425 889 611 432 336 315 311 275 57 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2262 3934 1537 Size: 275 450 1672 4090 Fragments arranged by size: 4090 1672 450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 2991 3566 4417 1353 Size: 1247 391 575 851 3423 Fragments arranged by size: 3423 1247 851 575 391 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 5074 5448 5594 387 Size: 1064 138 520 2965 374 146 1280 Fragments arranged by size: 2965 1280 1064 520 374 146 138 EcoICRI GAG'CTC Cuts at: 2911 2911 Size: 6487 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2218 2509 2642 3042 3497 3563 Size: 393 385 201 291 133 400 455 66 Cuts at: 3563 3746 4550 5147 5934 6202 1239 Size: 183 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 455 400 393 385 291 268 201 183 133 66 EcoNI CCTnn'n_nnAGG Cuts at: 3057 3057 Size: 6487 EcoO109I rG'GnC_Cy Cuts at: 2412 2453 2821 3207 3401 2412 Size: 41 368 386 194 5498 Fragments arranged by size: 5498 386 368 194 41 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 6487 FseI GG_CCGG'CC Cuts at: 2389 2389 Size: 6487 FspI TGC'GCA Cuts at: 2165 4993 2165 Size: 2828 3659 HaeII r_GCGC'y Cuts at: 1678 2162 2174 2351 2373 2429 5634 1678 Size: 484 12 177 22 56 3205 2531 Fragments arranged by size: 3205 2531 484 177 56 22 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2938 2970 3040 3072 5128 Size: 32 135 32 1366 32 70 32 2056 Cuts at: 5128 5160 5202 5234 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2056 1366 135 70 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3280 3831 4077 669 Size: 988 281 1342 551 246 3079 Fragments arranged by size: 3079 1342 988 551 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6487 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KasI G'GCGC_C Cuts at: 2170 2347 2425 2170 Size: 177 78 6232 Fragments arranged by size: 6232 177 78 KpnI G_GTAC'C Cuts at: 3505 3812 3505 Size: 307 6180 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5601 5785 1305 Size: 4296 184 2007 Fragments arranged by size: 4296 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3621 10 Size: 54 1425 343 1789 2876 Fragments arranged by size: 2876 1789 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3608 4445 4804 4963 518 Size: 648 1302 1140 837 359 159 2042 Fragments arranged by size: 2042 1302 1140 837 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2250 2301 2404 3903 4580 5728 5973 1606 Size: 644 51 103 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 644 245 103 51 NaeI GCC'GGC Cuts at: 2387 3312 2387 Size: 925 5562 NarI GG'CG_CC Cuts at: 2171 2348 2426 2171 Size: 177 78 6232 Fragments arranged by size: 6232 177 78 NcoI C'CATG_G Cuts at: 513 1065 2987 513 Size: 552 1922 4013 Fragments arranged by size: 4013 1922 552 NdeI CA'TA_TG Cuts at: 387 2809 387 Size: 2422 4065 NgoMIV G'CCGG_C Cuts at: 2385 3310 2385 Size: 925 5562 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 2755 3613 3845 5390 1132 Size: 97 1526 858 232 1545 2229 Fragments arranged by size: 2229 1545 1526 858 232 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 5386 5386 Size: 6487 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 3397 1108 Size: 1341 948 4198 Fragments arranged by size: 4198 1341 948 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PmlI CAC'GTG Cuts at: 3247 3247 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 3207 2821 Size: 386 6101 PshAI GACnn'nnGTC Cuts at: 1613 2054 1613 Size: 441 6046 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PspOMI G'GGCC_C Cuts at: 2183 2453 3401 2183 Size: 270 948 5269 Fragments arranged by size: 5269 948 270 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2527 2559 2527 Size: 32 6455 PstI C_TGCA'G Cuts at: 830 2306 3745 3839 830 Size: 1476 1439 94 3478 Fragments arranged by size: 3478 1476 1439 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 2301 2301 Size: 6487 SacI G_AGCT'C Cuts at: 2913 2913 Size: 6487 SacII CC_GC'GG Cuts at: 2251 2251 Size: 6487 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SapI GCTCTTCn'nnn_ Cuts at: 3566 3566 Size: 6487 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 2480 4735 6447 2480 Size: 2255 1712 2520 Fragments arranged by size: 2520 2255 1712 SexAI A'CCwGG_T Cuts at: 2442 2442 Size: 6487 SfcI C'TryA_G Cuts at: 826 1045 2302 3220 3741 3835 4970 5651 5842 Size: 219 1257 918 521 94 1135 681 191 Cuts at: 5842 826 Size: 1471 Fragments arranged by size: 1471 1257 1135 918 681 521 219 191 94 SfiI GGCCn_nnn'nGGCC Cuts at: 2250 2250 Size: 6487 SfoI GGC'GCC Cuts at: 2172 2349 2427 2172 Size: 177 78 6232 Fragments arranged by size: 6232 177 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 2270 3588 3814 1872 Size: 398 1318 226 4545 Fragments arranged by size: 4545 1318 398 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 3383 4591 5492 5754 820 Size: 197 926 1440 1208 901 262 1553 Fragments arranged by size: 1553 1440 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3613 3845 1132 Size: 2481 232 3774 Fragments arranged by size: 3774 2481 232 SspI AAT'ATT Cuts at: 5 52 2927 4411 5 Size: 47 2875 1484 2081 Fragments arranged by size: 2875 2081 1484 47 StuI AGG'CCT Cuts at: 1517 3305 1517 Size: 1788 4699 StyI C'CwwG_G Cuts at: 513 1065 2987 3341 3396 3505 3926 513 Size: 552 1922 354 55 109 421 3074 Fragments arranged by size: 3074 1922 552 421 354 109 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 3036 3283 4514 4531 4684 4869 Size: 1255 464 992 247 1231 17 153 185 Cuts at: 4869 5314 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1231 992 464 445 247 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 2528 4733 Size: 274 80 33 51 1443 501 50 2205 Cuts at: 4733 6445 96 Size: 1712 138 Fragments arranged by size: 2205 1712 1443 501 274 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 2741 2773 6447 6479 2201 Size: 32 508 32 3674 32 2209 Fragments arranged by size: 3674 2209 508 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3677 1316 Size: 2361 4126 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3303 3303 Size: 6487 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6487 XmaI C'CCGG_G Cuts at: 1870 2268 3586 3812 1870 Size: 398 1318 226 4545 Fragments arranged by size: 4545 1318 398 226 XmnI GAAnn'nnTTC Cuts at: 4616 4616 Size: 6487 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI PacI PfoI PspXI RsrII SanDI SrfI SwaI