################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 31 GCGG CCGC(-3/-1) AlwNI 157 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 94 GTGCTC G[AT]GC[AT]^C BalI 282 TGGCCA TGG^CCA BanII 381 GGGCTC G[GA]GC[CT]^C BbvI 147 GCTGC GCAGC(8/12) BfaI 403 CTAG C^TAG BpmI 51 CTGGAG CTGGAG(16/14) BsaJI 366 CCTTGG C^C[ACGT][ACGT]GG BsiHKAI 94 GTGCTC G[AT]GC[AT]^C BsmFI 215 GGGAC GGGAC(10/14) BspMI 170 ACCTGC ACCTGC(4/8) BstF5I 303 CATCC GGATG(2/0) Cfr13I 78 GGCCC G^G[ACGT]CC EcoT14I 366 CCTTGG C^C[AT][AT]GG EcoT22I 7 ATGCAT ATGCA^T Fnu4HI 147 GCTGC GC^[ACGT]GC FokI 303 CATCC GGATG(9/13) HphI 165 GGTGA GGTGA(8/7) ItaI 147 GCTGC GC^[ACGT]GC MaeI 403 CTAG C^TAG MaeIII 275 GTCAC ^GT[ACGT]AC MboII 17 TCTTC GAAGA(8/7) MluNI 282 TGGCCA TGG^CCA MnlI 179 CCTC CCTC(7/6) MscI 282 TGGCCA TGG^CCA MslI 303 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 29 CAGCGG C[AC]G^C[GT]G MwoI 70 GCTGGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 7 ATGCAT ATGCA^T PflMI 156 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 217 GACTC GAGTC(4/5) PstI 221 CTGCAG CTGCA^G Sau96I 78 GGCCC G^G[ACGT]CC SexAI 155 ACCAGGT A^CC[AT]GGT SfcI 221 CTGCAG C^T[GA][CT]AG StyI 366 CCTTGG C^C[AT][AT]GG TfiI 232 GATTC G^A[AT]TC TseI 147 GCTGC G^C[AT]GC Tsp45I 275 GTCAC ^GT[GC]AC Van91I 156 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 60 CCACAGTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AfaI 112 GTAC GT^AC 160 GTAC BmyI 94 GTGCTC G[AGT]GC[ACT]^C 381 GGGCTC BsmAI 205 GAGAC GTCTC(1/5) 288 GTCTC Bsp1286I 94 GTGCTC G[AGT]GC[ACT]^C 381 GGGCTC BsrI 162 ACTGG ACTGG(1/-1) 285 CCAGT EaeI 282 TGGCCA [CT]^GGCC[GA] 335 TGGCCG HinfI 217 GACTC G^A[ACGT]TC 232 GATTC RsaI 112 GTAC GT^AC 160 GTAC SfaNI 6 GATGC GCATC(5/9) 125 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BsiYI BslI BssKI BstNI Cac8I DdeI EcoRII HaeIII MvaI NlaIII ScrFI