################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BanII BbrPI BbsI BbvCI BciVI BclI BglI BpmI BpuAI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I Cfr10I CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT22I FauI FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MluI MluNI MroI MscI MslI MspA1I MvaI MvnI MwoI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 49 GGCGCC G[GA]^CG[CT]C AflII 645 CTTAAG C^TTAAG AlwI 795 GGATC GGATC(4/5) AspHI 106 GAGCAC G[AT]GC[AT]^C AvaI 1103 CTCGGG C^[CT]CG[GA]G AvrII 220 CCTAGG C^CTAGG BanI 49 GGCGCC G^G[CT][GA]CC BbeI 49 GGCGCC GGCGC^C BcgI 900 CGACCTTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 828 CCGGG CC^[GC]GG BfrI 645 CTTAAG C^TTAAG BglII 255 AGATCT A^GATCT BlnI 220 CCTAGG C^CTAGG BlpI 248 GCTTAGC GC^T[ACGT]AGC BmyI 106 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 248 GCTTAGC GC^T[ACGT]AGC BsaHI 49 GGCGCC G[GA]^CG[CT]C BsaWI 96 TCCGGT [AT]^CCGG[AT] BsgI 675 GTGCAG GTGCAG(16/14) BsiHKAI 106 GAGCAC G[AT]GC[AT]^C BsoBI 1103 CTCGGG C^[CT]CG[GA]G Bsp1286I 106 GAGCAC G[AGT]GC[ACT]^C BspDI 806 ATCGAT AT^CGAT BspHI 972 TCATGA T^CATGA BspMI 139 ACCTGC ACCTGC(4/8) BssKI 828 CCGGG ^CC[ACGT]GG BstYI 255 AGATCT [GA]^GATC[CT] CelII 248 GCTTAGC GC^T[ACGT]AGC CfoI 50 GCGC GCG^C ClaI 806 ATCGAT AT^CGAT DraI 784 TTTAAA TTT^AAA DsaI 44 CCATGG C^C[GA][CT]GG EcoRV 1057 GATATC GAT^ATC HaeII 49 GGCGCC [GA]GCGC^[CT] HaeIII 946 GGCC GG^CC HhaI 50 GCGC GCG^C Hin1I 49 GGCGCC G[GA]^CG[CT]C HinP1I 50 GCGC G^CGC HphI 871 GGTGA GGTGA(8/7) KasI 49 GGCGCC G^GCGCC MaeIII 1082 GTTAC ^GT[ACGT]AC MfeI 404 CAATTG C^AATTG MflI 255 AGATCT [GA]^GATC[CT] MunI 404 CAATTG C^AATTG NarI 49 GGCGCC GG^CGCC NciI 828 CCGGG CC^[GC]GG NcoI 44 CCATGG C^CATGG RcaI 972 TCATGA T^CATGA ScrFI 828 CCGGG CC^[ACGT]GG SfoI 49 GGCGCC GGC^GCC SmlI 645 CTTAAG C^T[CT][GA]AG SspI 469 AATATT AAT^ATT SwaI 783 ATTTAAAT ATTT^AAAT TfiI 759 GATTC G^A[AT]TC XhoII 255 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AfaI 41 GTAC GT^AC 897 GTAC Asp700I 88 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 193 GAAAAAGTTC AvaII 82 GGTCC G^G[AT]CC 934 GGTCC BbvI 578 GCAGC GCAGC(8/12) 710 GCAGC BfaI 221 CTAG C^TAG 763 CTAG BsaJI 44 CCATGG C^C[ACGT][ACGT]GG 220 CCTAGG BsiYI 504 CCCAACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 937 CCCAATCGAGG BslI 504 CCCAACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 937 CCCAATCGAGG BsmAI 19 GTCTC GTCTC(1/5) 1101 GTCTC BsmFI 1106 GGGAC GGGAC(10/14) 935 GTCCC BstF5I 1086 CATCC GGATG(2/0) 734 GGATG Cfr13I 82 GGTCC G^G[ACGT]CC 934 GGTCC DpnII 256 GATC ^GATC 796 GATC EcoRI 379 GAATTC G^AATTC 1036 GAATTC EcoT14I 44 CCATGG C^C[AT][AT]GG 220 CCTAGG Fnu4HI 578 GCAGC GC^[ACGT]GC 710 GCAGC FokI 1086 CATCC GGATG(9/13) 734 GGATG HapII 97 CCGG C^CGG 828 CCGG HpaII 97 CCGG C^CGG 828 CCGG ItaI 578 GCAGC GC^[ACGT]GC 710 GCAGC MaeI 221 CTAG C^TAG 763 CTAG MaeII 508 ACGT A^CGT 614 ACGT MboI 256 GATC ^GATC 796 GATC MnlI 32 CCTC CCTC(7/6) 944 GAGG MspI 97 CCGG C^CGG 828 CCGG NdeII 256 GATC ^GATC 796 GATC NlaIV 49 GGCGCC GG[ACGT]^[ACGT]CC 934 GGTCCC NspI 244 ACATGC [GA]CATG^[CT] 359 ACATGC RsaI 41 GTAC GT^AC 897 GTAC Sau3AI 256 GATC ^GATC 796 GATC Sau96I 82 GGTCC G^G[ACGT]CC 934 GGTCC StyI 44 CCATGG C^C[AT][AT]GG 220 CCTAGG TaqI 807 TCGA T^CGA 942 TCGA TseI 578 GCAGC G^C[AT]GC 710 GCAGC TthHB8I 807 TCGA T^CGA 942 TCGA XcmI 35 CCATCTGTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 499 CCAGACCCAACGTGG XmnI 88 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 193 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AcsI AluI ApoI DdeI EarI HincII HindII HinfI Ksp632I MboII MseI NlaIII PleI SfaNI Tru9I Tsp509I TspRI