Restriction map for
pFN21AE3673
(Linear) (Six-base) MAPSORT of: KIEE3673.seq Check: 4294 from: 1 to: 1110
KIEE3673.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 851 1110
Size: 796 55 259
Fragments arranged by size:
796 259 55
AflII C'TTAA_G
Cuts at: 0 645 1110
Size: 645 465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 386 1110
Size: 386 724
ApoI r'AATT_y
Cuts at: 0 25 379 1036 1110
Size: 25 354 657 74
Fragments arranged by size:
657 354 74 25
AvaI C'yCGr_G
Cuts at: 0 1103 1110
Size: 1103 7
AvrII C'CTAG_G
Cuts at: 0 220 1110
Size: 220 890
BanI G'GyrC_C
Cuts at: 0 49 1110
Size: 49 1061
BbeI G_GCGC'C
Cuts at: 0 53 1110
Size: 53 1057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 923 1110
Size: 889 34 187
Fragments arranged by size:
889 187 34
BglII A'GATC_T
Cuts at: 0 255 1110
Size: 255 855
BlpI GC'TnA_GC
Cuts at: 0 249 1110
Size: 249 861
BmgBI CAC'GTC
Cuts at: 0 615 1110
Size: 615 495
BsaHI Gr'CG_yC
Cuts at: 0 50 1110
Size: 50 1060
BsaWI w'CCGG_w
Cuts at: 0 96 1110
Size: 96 1014
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1110
Size: 696 414
BsiHKAI G_wGCw'C
Cuts at: 0 110 1110
Size: 110 1000
Bsp1286I G_dGCh'C
Cuts at: 0 110 1110
Size: 110 1000
BspHI T'CATG_A
Cuts at: 0 972 1110
Size: 972 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 148 1110
Size: 148 962
BstYI r'GATC_y
Cuts at: 0 255 1110
Size: 255 855
BtgI C'CryG_G
Cuts at: 0 44 1110
Size: 44 1066
BtsI GCAGTG_nn'
Cuts at: 0 639 684 1110
Size: 639 45 426
Fragments arranged by size:
639 426 45
ClaI AT'CG_AT
Cuts at: 0 807 1110
Size: 807 303
DraI TTT'AAA
Cuts at: 0 786 1110
Size: 786 324
EarI CTCTTCn'nnn_
Cuts at: 0 265 419 695 772 1110
Size: 265 154 276 77 338
Fragments arranged by size:
338 276 265 154 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 851 1110
Size: 796 55 259
Fragments arranged by size:
796 259 55
EcoRI G'AATT_C
Cuts at: 0 379 1036 1110
Size: 379 657 74
Fragments arranged by size:
657 379 74
EcoRV GAT'ATC
Cuts at: 0 1059 1110
Size: 1059 51
HaeII r_GCGC'y
Cuts at: 0 53 1110
Size: 53 1057
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 750 782 1110
Size: 750 32 328
Fragments arranged by size:
750 328 32
HincII GTy'rAC
Cuts at: 0 217 612 1049 1110
Size: 217 395 437 61
Fragments arranged by size:
437 395 217 61
KasI G'GCGC_C
Cuts at: 0 49 1110
Size: 49 1061
MfeI C'AATT_G
Cuts at: 0 404 1110
Size: 404 706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1110
Size: 273 837
NarI GG'CG_CC
Cuts at: 0 50 1110
Size: 50 1060
NcoI C'CATG_G
Cuts at: 0 44 1110
Size: 44 1066
NspI r_CATG'y
Cuts at: 0 248 363 1110
Size: 248 115 747
Fragments arranged by size:
747 248 115
SfoI GGC'GCC
Cuts at: 0 51 1110
Size: 51 1059
SmlI C'TyrA_G
Cuts at: 0 645 1110
Size: 645 465
SspI AAT'ATT
Cuts at: 0 471 1110
Size: 471 639
StyI C'CwwG_G
Cuts at: 0 44 220 1110
Size: 44 176 890
Fragments arranged by size:
890 176 44
SwaI ATTT'AAAT
Cuts at: 0 786 1110
Size: 786 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 506 1110
Size: 42 464 604
Fragments arranged by size:
604 464 42
XmnI GAAnn'nnTTC
Cuts at: 0 92 197 1110
Size: 92 105 913
Fragments arranged by size:
913 105 92
Enzymes that do cut:
AcuI AflII AlfI ApoI AvaI AvrII BanI BbeI
BcgI BglII BlpI BmgBI BsaHI BsaWI BsgI BsiHKAI
Bsp1286I BspHI BspMI BstYI BtgI BtsI ClaI DraI
EarI Eco57MI EcoRI EcoRV HaeII Hin4I HincII KasI
MfeI MmeI NarI NcoI NspI SfoI SmlI SspI
StyI SwaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BanII BbsI BbvCI BciVI BclI
BfrBI BglI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I FalI FseI FspI FspAI HindIII HpaI
KpnI MluI MscI MslI MspA1I NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI